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Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

BACKGROUND: Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated...

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Autores principales: das Neves Selis, Nathan, de Oliveira, Hellen Braga Martins, Leão, Hiago Ferreira, dos Anjos, Yan Bento, Sampaio, Beatriz Almeida, Correia, Thiago Macêdo Lopes, Almeida, Carolline Florentino, Pena, Larissa Silva Carvalho, Reis, Mariane Mares, Brito, Thamara Louisy Santos, Brito, Laís Ferraz, Campos, Guilherme Barreto, Timenetsky, Jorge, Cruz, Mariluze Peixoto, Rezende, Rachel Passos, Romano, Carla Cristina, da Costa, Andréa Miura, Yatsuda, Regiane, Uetanabaro, Ana Paula Trovatti, Marques, Lucas Miranda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243870/
https://www.ncbi.nlm.nih.gov/pubmed/34187371
http://dx.doi.org/10.1186/s12866-021-02264-5
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author das Neves Selis, Nathan
de Oliveira, Hellen Braga Martins
Leão, Hiago Ferreira
dos Anjos, Yan Bento
Sampaio, Beatriz Almeida
Correia, Thiago Macêdo Lopes
Almeida, Carolline Florentino
Pena, Larissa Silva Carvalho
Reis, Mariane Mares
Brito, Thamara Louisy Santos
Brito, Laís Ferraz
Campos, Guilherme Barreto
Timenetsky, Jorge
Cruz, Mariluze Peixoto
Rezende, Rachel Passos
Romano, Carla Cristina
da Costa, Andréa Miura
Yatsuda, Regiane
Uetanabaro, Ana Paula Trovatti
Marques, Lucas Miranda
author_facet das Neves Selis, Nathan
de Oliveira, Hellen Braga Martins
Leão, Hiago Ferreira
dos Anjos, Yan Bento
Sampaio, Beatriz Almeida
Correia, Thiago Macêdo Lopes
Almeida, Carolline Florentino
Pena, Larissa Silva Carvalho
Reis, Mariane Mares
Brito, Thamara Louisy Santos
Brito, Laís Ferraz
Campos, Guilherme Barreto
Timenetsky, Jorge
Cruz, Mariluze Peixoto
Rezende, Rachel Passos
Romano, Carla Cristina
da Costa, Andréa Miura
Yatsuda, Regiane
Uetanabaro, Ana Paula Trovatti
Marques, Lucas Miranda
author_sort das Neves Selis, Nathan
collection PubMed
description BACKGROUND: Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. RESULTS: The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. CONCLUSION: The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.
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spelling pubmed-82438702021-06-30 Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae das Neves Selis, Nathan de Oliveira, Hellen Braga Martins Leão, Hiago Ferreira dos Anjos, Yan Bento Sampaio, Beatriz Almeida Correia, Thiago Macêdo Lopes Almeida, Carolline Florentino Pena, Larissa Silva Carvalho Reis, Mariane Mares Brito, Thamara Louisy Santos Brito, Laís Ferraz Campos, Guilherme Barreto Timenetsky, Jorge Cruz, Mariluze Peixoto Rezende, Rachel Passos Romano, Carla Cristina da Costa, Andréa Miura Yatsuda, Regiane Uetanabaro, Ana Paula Trovatti Marques, Lucas Miranda BMC Microbiol Research Article BACKGROUND: Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. RESULTS: The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. CONCLUSION: The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections. BioMed Central 2021-06-29 /pmc/articles/PMC8243870/ /pubmed/34187371 http://dx.doi.org/10.1186/s12866-021-02264-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
das Neves Selis, Nathan
de Oliveira, Hellen Braga Martins
Leão, Hiago Ferreira
dos Anjos, Yan Bento
Sampaio, Beatriz Almeida
Correia, Thiago Macêdo Lopes
Almeida, Carolline Florentino
Pena, Larissa Silva Carvalho
Reis, Mariane Mares
Brito, Thamara Louisy Santos
Brito, Laís Ferraz
Campos, Guilherme Barreto
Timenetsky, Jorge
Cruz, Mariluze Peixoto
Rezende, Rachel Passos
Romano, Carla Cristina
da Costa, Andréa Miura
Yatsuda, Regiane
Uetanabaro, Ana Paula Trovatti
Marques, Lucas Miranda
Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae
title Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae
title_full Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae
title_fullStr Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae
title_full_unstemmed Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae
title_short Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae
title_sort lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against gardnerella vaginalis and neisseria gonorrhoeae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243870/
https://www.ncbi.nlm.nih.gov/pubmed/34187371
http://dx.doi.org/10.1186/s12866-021-02264-5
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