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Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility

OBJECTIVE: To study decidualization-associated endometrial factors. DESIGN: Retrospective cohort study to compare endometrial gene expression patterns in women experiencing reproductive failure including recurrent pregnancy loss or unexplained infertility versus fertile controls. SETTING: University...

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Autores principales: Dambaeva, Svetlana, Bilal, Mahmood, Schneiderman, Sylvia, Germain, Alfredo, Fernandez, Emilio, Kwak-Kim, Joanne, Beaman, Kenneth, Coulam, Carolyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244268/
https://www.ncbi.nlm.nih.gov/pubmed/34223279
http://dx.doi.org/10.1016/j.xfre.2020.12.004
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author Dambaeva, Svetlana
Bilal, Mahmood
Schneiderman, Sylvia
Germain, Alfredo
Fernandez, Emilio
Kwak-Kim, Joanne
Beaman, Kenneth
Coulam, Carolyn
author_facet Dambaeva, Svetlana
Bilal, Mahmood
Schneiderman, Sylvia
Germain, Alfredo
Fernandez, Emilio
Kwak-Kim, Joanne
Beaman, Kenneth
Coulam, Carolyn
author_sort Dambaeva, Svetlana
collection PubMed
description OBJECTIVE: To study decidualization-associated endometrial factors. DESIGN: Retrospective cohort study to compare endometrial gene expression patterns in women experiencing reproductive failure including recurrent pregnancy loss or unexplained infertility versus fertile controls. SETTING: University Reproductive Medicine Center. PATIENT(S): Women experiencing recurrent reproductive failure including recurrent pregnancy loss or unexplained infertility (n = 42) and fertile controls (n = 18). INTERVENTION(S): Endometrial biopsy samples were analyzed with targeted ribonucleic acid sequencing via next-generation sequencing. MAIN OUTCOME MEASURE(S): The primary end point measurements were the expression of genes important for endometrial transformation during decidualization measured singly and in a combined/cumulative score approach. The secondary end point measurements were receiver operating curve analysis and comparisons between the specific biomarkers. RESULT(S): The comparison revealed differential expression of factors associated with decidualization, tissue homeostasis, and immune regulation: FOXO1, GZMB, IL15, SCNN1A, SGK1, and SLC2A1. A combined evaluation of these 6 signature factors was designated as a decidualization score in which the maximal score was “6” and the minimal was “0”. Among controls, 89% of the samples had a score ≥5 and 11% had a score of “4”. A total of 76% of samples in the patient group had scores ≤4 and 19% had the lowest score of “0”. A decidualization score <4 provided evidence of abnormality in the decidualization process with a sensitivity of 76% (95% CI 61%-88%) and specificity of 89% (95% CI 65%-99%). CONCLUSION(S): Decidualization scoring can determine whether the endometrial molecular profile is implantation-friendly. Further validation of this testing approach is necessary to determine a particular patient population in whom it could be used for selecting patients that require therapeutic actions to improve endometrial conditions prior to the in vitro fertilization procedure
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spelling pubmed-82442682021-07-02 Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility Dambaeva, Svetlana Bilal, Mahmood Schneiderman, Sylvia Germain, Alfredo Fernandez, Emilio Kwak-Kim, Joanne Beaman, Kenneth Coulam, Carolyn F S Rep Original Article OBJECTIVE: To study decidualization-associated endometrial factors. DESIGN: Retrospective cohort study to compare endometrial gene expression patterns in women experiencing reproductive failure including recurrent pregnancy loss or unexplained infertility versus fertile controls. SETTING: University Reproductive Medicine Center. PATIENT(S): Women experiencing recurrent reproductive failure including recurrent pregnancy loss or unexplained infertility (n = 42) and fertile controls (n = 18). INTERVENTION(S): Endometrial biopsy samples were analyzed with targeted ribonucleic acid sequencing via next-generation sequencing. MAIN OUTCOME MEASURE(S): The primary end point measurements were the expression of genes important for endometrial transformation during decidualization measured singly and in a combined/cumulative score approach. The secondary end point measurements were receiver operating curve analysis and comparisons between the specific biomarkers. RESULT(S): The comparison revealed differential expression of factors associated with decidualization, tissue homeostasis, and immune regulation: FOXO1, GZMB, IL15, SCNN1A, SGK1, and SLC2A1. A combined evaluation of these 6 signature factors was designated as a decidualization score in which the maximal score was “6” and the minimal was “0”. Among controls, 89% of the samples had a score ≥5 and 11% had a score of “4”. A total of 76% of samples in the patient group had scores ≤4 and 19% had the lowest score of “0”. A decidualization score <4 provided evidence of abnormality in the decidualization process with a sensitivity of 76% (95% CI 61%-88%) and specificity of 89% (95% CI 65%-99%). CONCLUSION(S): Decidualization scoring can determine whether the endometrial molecular profile is implantation-friendly. Further validation of this testing approach is necessary to determine a particular patient population in whom it could be used for selecting patients that require therapeutic actions to improve endometrial conditions prior to the in vitro fertilization procedure Elsevier 2020-12-29 /pmc/articles/PMC8244268/ /pubmed/34223279 http://dx.doi.org/10.1016/j.xfre.2020.12.004 Text en © 2020 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Dambaeva, Svetlana
Bilal, Mahmood
Schneiderman, Sylvia
Germain, Alfredo
Fernandez, Emilio
Kwak-Kim, Joanne
Beaman, Kenneth
Coulam, Carolyn
Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
title Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
title_full Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
title_fullStr Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
title_full_unstemmed Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
title_short Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
title_sort decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244268/
https://www.ncbi.nlm.nih.gov/pubmed/34223279
http://dx.doi.org/10.1016/j.xfre.2020.12.004
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