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Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility
OBJECTIVE: To study decidualization-associated endometrial factors. DESIGN: Retrospective cohort study to compare endometrial gene expression patterns in women experiencing reproductive failure including recurrent pregnancy loss or unexplained infertility versus fertile controls. SETTING: University...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244268/ https://www.ncbi.nlm.nih.gov/pubmed/34223279 http://dx.doi.org/10.1016/j.xfre.2020.12.004 |
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author | Dambaeva, Svetlana Bilal, Mahmood Schneiderman, Sylvia Germain, Alfredo Fernandez, Emilio Kwak-Kim, Joanne Beaman, Kenneth Coulam, Carolyn |
author_facet | Dambaeva, Svetlana Bilal, Mahmood Schneiderman, Sylvia Germain, Alfredo Fernandez, Emilio Kwak-Kim, Joanne Beaman, Kenneth Coulam, Carolyn |
author_sort | Dambaeva, Svetlana |
collection | PubMed |
description | OBJECTIVE: To study decidualization-associated endometrial factors. DESIGN: Retrospective cohort study to compare endometrial gene expression patterns in women experiencing reproductive failure including recurrent pregnancy loss or unexplained infertility versus fertile controls. SETTING: University Reproductive Medicine Center. PATIENT(S): Women experiencing recurrent reproductive failure including recurrent pregnancy loss or unexplained infertility (n = 42) and fertile controls (n = 18). INTERVENTION(S): Endometrial biopsy samples were analyzed with targeted ribonucleic acid sequencing via next-generation sequencing. MAIN OUTCOME MEASURE(S): The primary end point measurements were the expression of genes important for endometrial transformation during decidualization measured singly and in a combined/cumulative score approach. The secondary end point measurements were receiver operating curve analysis and comparisons between the specific biomarkers. RESULT(S): The comparison revealed differential expression of factors associated with decidualization, tissue homeostasis, and immune regulation: FOXO1, GZMB, IL15, SCNN1A, SGK1, and SLC2A1. A combined evaluation of these 6 signature factors was designated as a decidualization score in which the maximal score was “6” and the minimal was “0”. Among controls, 89% of the samples had a score ≥5 and 11% had a score of “4”. A total of 76% of samples in the patient group had scores ≤4 and 19% had the lowest score of “0”. A decidualization score <4 provided evidence of abnormality in the decidualization process with a sensitivity of 76% (95% CI 61%-88%) and specificity of 89% (95% CI 65%-99%). CONCLUSION(S): Decidualization scoring can determine whether the endometrial molecular profile is implantation-friendly. Further validation of this testing approach is necessary to determine a particular patient population in whom it could be used for selecting patients that require therapeutic actions to improve endometrial conditions prior to the in vitro fertilization procedure |
format | Online Article Text |
id | pubmed-8244268 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-82442682021-07-02 Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility Dambaeva, Svetlana Bilal, Mahmood Schneiderman, Sylvia Germain, Alfredo Fernandez, Emilio Kwak-Kim, Joanne Beaman, Kenneth Coulam, Carolyn F S Rep Original Article OBJECTIVE: To study decidualization-associated endometrial factors. DESIGN: Retrospective cohort study to compare endometrial gene expression patterns in women experiencing reproductive failure including recurrent pregnancy loss or unexplained infertility versus fertile controls. SETTING: University Reproductive Medicine Center. PATIENT(S): Women experiencing recurrent reproductive failure including recurrent pregnancy loss or unexplained infertility (n = 42) and fertile controls (n = 18). INTERVENTION(S): Endometrial biopsy samples were analyzed with targeted ribonucleic acid sequencing via next-generation sequencing. MAIN OUTCOME MEASURE(S): The primary end point measurements were the expression of genes important for endometrial transformation during decidualization measured singly and in a combined/cumulative score approach. The secondary end point measurements were receiver operating curve analysis and comparisons between the specific biomarkers. RESULT(S): The comparison revealed differential expression of factors associated with decidualization, tissue homeostasis, and immune regulation: FOXO1, GZMB, IL15, SCNN1A, SGK1, and SLC2A1. A combined evaluation of these 6 signature factors was designated as a decidualization score in which the maximal score was “6” and the minimal was “0”. Among controls, 89% of the samples had a score ≥5 and 11% had a score of “4”. A total of 76% of samples in the patient group had scores ≤4 and 19% had the lowest score of “0”. A decidualization score <4 provided evidence of abnormality in the decidualization process with a sensitivity of 76% (95% CI 61%-88%) and specificity of 89% (95% CI 65%-99%). CONCLUSION(S): Decidualization scoring can determine whether the endometrial molecular profile is implantation-friendly. Further validation of this testing approach is necessary to determine a particular patient population in whom it could be used for selecting patients that require therapeutic actions to improve endometrial conditions prior to the in vitro fertilization procedure Elsevier 2020-12-29 /pmc/articles/PMC8244268/ /pubmed/34223279 http://dx.doi.org/10.1016/j.xfre.2020.12.004 Text en © 2020 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Dambaeva, Svetlana Bilal, Mahmood Schneiderman, Sylvia Germain, Alfredo Fernandez, Emilio Kwak-Kim, Joanne Beaman, Kenneth Coulam, Carolyn Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility |
title | Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility |
title_full | Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility |
title_fullStr | Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility |
title_full_unstemmed | Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility |
title_short | Decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility |
title_sort | decidualization score identifies an endometrial dysregulation in samples from women with recurrent pregnancy losses and unexplained infertility |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244268/ https://www.ncbi.nlm.nih.gov/pubmed/34223279 http://dx.doi.org/10.1016/j.xfre.2020.12.004 |
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