Cargando…

Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil

Fourier transform infrared spectroscopy (FTIR) spectroscopy detects functional groups such as vibrational bands like N-H, O-H, C-H, C = O (ester, amine, ketone, aldehyde), C = C, C = N (vibrational modes of a tetrapyrrole ring) and simply C = N. The FTIR of these bands is fundamental to the investig...

Descripción completa

Detalles Bibliográficos
Autores principales: Younis, Uzma, Rahi, Ashfaq Ahmad, Danish, Subhan, Ali, Muhammad Arif, Ahmed, Niaz, Datta, Rahul, Fahad, Shah, Holatko, Jiri, Hammerschmiedt, Tereza, Brtnicky, Martin, Zarei, Tayebeh, Baazeem, Alaa, Sabagh, Ayman EL, Glick, Bernard R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244852/
https://www.ncbi.nlm.nih.gov/pubmed/34191839
http://dx.doi.org/10.1371/journal.pone.0253390
_version_ 1783716006105972736
author Younis, Uzma
Rahi, Ashfaq Ahmad
Danish, Subhan
Ali, Muhammad Arif
Ahmed, Niaz
Datta, Rahul
Fahad, Shah
Holatko, Jiri
Hammerschmiedt, Tereza
Brtnicky, Martin
Zarei, Tayebeh
Baazeem, Alaa
Sabagh, Ayman EL
Glick, Bernard R.
author_facet Younis, Uzma
Rahi, Ashfaq Ahmad
Danish, Subhan
Ali, Muhammad Arif
Ahmed, Niaz
Datta, Rahul
Fahad, Shah
Holatko, Jiri
Hammerschmiedt, Tereza
Brtnicky, Martin
Zarei, Tayebeh
Baazeem, Alaa
Sabagh, Ayman EL
Glick, Bernard R.
author_sort Younis, Uzma
collection PubMed
description Fourier transform infrared spectroscopy (FTIR) spectroscopy detects functional groups such as vibrational bands like N-H, O-H, C-H, C = O (ester, amine, ketone, aldehyde), C = C, C = N (vibrational modes of a tetrapyrrole ring) and simply C = N. The FTIR of these bands is fundamental to the investigation of the effect of biochar (BC) treatment on structural changes in the chlorophyll molecules of both plants that were tested. For this, dried leaf of Spinacia oleracia (spinach) and Trigonella corniculata (fenugreek) were selected for FTIR spectral study of chlorophyll associated functional groups. The study’s primary goal was to investigate the silent features of infrared (IR) spectra of dried leave samples. The data obtained from the current study also shows that leaf chlorophyll can mask or suppress other molecules’ FITR bands, including proteins. In addition, the C = O bands with Mg and the C9 ketonic group of chlorophyll are observed as peaks at1600 (0%BC), 1650 (3%BC) and 1640, or near to1700 (5%BC) in spinach samples. In fenugreek, additional effects are observed in the FTIR spectra of chlorophyll at the major groups of C = C, C = O and C9 of the ketonic groups, and the vibrational bands are more evident at C-H and N-H of the tetrapyrrole ring. It is concluded that C-N bands are more visible in 5% BC treated spinach and fenugreek than in all other treatments. These types of spectra are useful in detecting changes or visibility of functional groups, which are very helpful in supporting biochemical data such as an increase in protein can be detected by more visibility of C-N bands in FTIR spectra.
format Online
Article
Text
id pubmed-8244852
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-82448522021-07-12 Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil Younis, Uzma Rahi, Ashfaq Ahmad Danish, Subhan Ali, Muhammad Arif Ahmed, Niaz Datta, Rahul Fahad, Shah Holatko, Jiri Hammerschmiedt, Tereza Brtnicky, Martin Zarei, Tayebeh Baazeem, Alaa Sabagh, Ayman EL Glick, Bernard R. PLoS One Research Article Fourier transform infrared spectroscopy (FTIR) spectroscopy detects functional groups such as vibrational bands like N-H, O-H, C-H, C = O (ester, amine, ketone, aldehyde), C = C, C = N (vibrational modes of a tetrapyrrole ring) and simply C = N. The FTIR of these bands is fundamental to the investigation of the effect of biochar (BC) treatment on structural changes in the chlorophyll molecules of both plants that were tested. For this, dried leaf of Spinacia oleracia (spinach) and Trigonella corniculata (fenugreek) were selected for FTIR spectral study of chlorophyll associated functional groups. The study’s primary goal was to investigate the silent features of infrared (IR) spectra of dried leave samples. The data obtained from the current study also shows that leaf chlorophyll can mask or suppress other molecules’ FITR bands, including proteins. In addition, the C = O bands with Mg and the C9 ketonic group of chlorophyll are observed as peaks at1600 (0%BC), 1650 (3%BC) and 1640, or near to1700 (5%BC) in spinach samples. In fenugreek, additional effects are observed in the FTIR spectra of chlorophyll at the major groups of C = C, C = O and C9 of the ketonic groups, and the vibrational bands are more evident at C-H and N-H of the tetrapyrrole ring. It is concluded that C-N bands are more visible in 5% BC treated spinach and fenugreek than in all other treatments. These types of spectra are useful in detecting changes or visibility of functional groups, which are very helpful in supporting biochemical data such as an increase in protein can be detected by more visibility of C-N bands in FTIR spectra. Public Library of Science 2021-06-30 /pmc/articles/PMC8244852/ /pubmed/34191839 http://dx.doi.org/10.1371/journal.pone.0253390 Text en © 2021 Younis et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Younis, Uzma
Rahi, Ashfaq Ahmad
Danish, Subhan
Ali, Muhammad Arif
Ahmed, Niaz
Datta, Rahul
Fahad, Shah
Holatko, Jiri
Hammerschmiedt, Tereza
Brtnicky, Martin
Zarei, Tayebeh
Baazeem, Alaa
Sabagh, Ayman EL
Glick, Bernard R.
Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil
title Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil
title_full Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil
title_fullStr Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil
title_full_unstemmed Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil
title_short Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil
title_sort fourier transform infrared spectroscopy vibrational bands study of spinacia oleracea and trigonella corniculata under biochar amendment in naturally contaminated soil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244852/
https://www.ncbi.nlm.nih.gov/pubmed/34191839
http://dx.doi.org/10.1371/journal.pone.0253390
work_keys_str_mv AT younisuzma fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT rahiashfaqahmad fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT danishsubhan fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT alimuhammadarif fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT ahmedniaz fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT dattarahul fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT fahadshah fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT holatkojiri fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT hammerschmiedttereza fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT brtnickymartin fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT zareitayebeh fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT baazeemalaa fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT sabaghaymanel fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil
AT glickbernardr fouriertransforminfraredspectroscopyvibrationalbandsstudyofspinaciaoleraceaandtrigonellacorniculataunderbiocharamendmentinnaturallycontaminatedsoil