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Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits

This study was designed to investigate the effect of ammonia on growth performance, lipid metabolism and intestinal flora of rabbits. A total of 150 female IRA rabbits (35-days-old) were randomly divided into three groups including 0 ppm (CG), 10 ppm (LAC) and 30 ppm ammonia (HAC) groups for a perio...

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Autores principales: Cui, Jia, Yang, Xinyu, Wang, Fengxia, Liu, Shudong, Han, Shuaijuan, Chen, Baojiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244895/
https://www.ncbi.nlm.nih.gov/pubmed/34191811
http://dx.doi.org/10.1371/journal.pone.0252065
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author Cui, Jia
Yang, Xinyu
Wang, Fengxia
Liu, Shudong
Han, Shuaijuan
Chen, Baojiang
author_facet Cui, Jia
Yang, Xinyu
Wang, Fengxia
Liu, Shudong
Han, Shuaijuan
Chen, Baojiang
author_sort Cui, Jia
collection PubMed
description This study was designed to investigate the effect of ammonia on growth performance, lipid metabolism and intestinal flora of rabbits. A total of 150 female IRA rabbits (35-days-old) were randomly divided into three groups including 0 ppm (CG), 10 ppm (LAC) and 30 ppm ammonia (HAC) groups for a period of 28 days. The average daily weight gain (ADG) of rabbits was significantly reduced in LAC (-17.11%; p < 0.001) and HAC groups (-17.46%; p < 0.001) as compared with the CG. Serum concentration of high density lipoprotein (HDL) and glucose (Glu) were increased in LAC (+80.95%; +45.99; p < 0.05) and HAC groups (+219.05%; +45.89; p < 0.001), while apolipoprotein A1 (apoA1) was decreased in LAC (-58.49%; p < 0.001) and HAC groups (-36.92%; p < 0.001). The structural integrity of cecum was damaged, and the thickness of mucosa and serosa were significantly decreased in LAC and HAC. The acetate, butyrate and propionate level of cecal chyme were reduced in HAC group (-21.67%; -19.82%; -30.81%; p < 0.05). Microbial diversity and burden of Firmicutes were significantly decreased, while that of pathogenic bacteria, such as Bacteroidetes, Clostridium and Proteobacteria were increased in ammonia treated groups. Spearman’s correlation confirmed that burden of Ruminococcaceae_NK4A214_group showed significantly negative correlation with acetic acid (r = -0.67; p < 0.001) while Barnesiellaceae_unclassified showed significantly positive correlation with propionic acid (r = 0.50; p < 0.001). In conclusion, ammonia treatment was responsible for an imbalance of intestinal flora, which affected lipid metabolism and damaged intestinal barrier of rabbits, resulting in low growth performance due to lipid metabolism dysfunction.
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spelling pubmed-82448952021-07-12 Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits Cui, Jia Yang, Xinyu Wang, Fengxia Liu, Shudong Han, Shuaijuan Chen, Baojiang PLoS One Research Article This study was designed to investigate the effect of ammonia on growth performance, lipid metabolism and intestinal flora of rabbits. A total of 150 female IRA rabbits (35-days-old) were randomly divided into three groups including 0 ppm (CG), 10 ppm (LAC) and 30 ppm ammonia (HAC) groups for a period of 28 days. The average daily weight gain (ADG) of rabbits was significantly reduced in LAC (-17.11%; p < 0.001) and HAC groups (-17.46%; p < 0.001) as compared with the CG. Serum concentration of high density lipoprotein (HDL) and glucose (Glu) were increased in LAC (+80.95%; +45.99; p < 0.05) and HAC groups (+219.05%; +45.89; p < 0.001), while apolipoprotein A1 (apoA1) was decreased in LAC (-58.49%; p < 0.001) and HAC groups (-36.92%; p < 0.001). The structural integrity of cecum was damaged, and the thickness of mucosa and serosa were significantly decreased in LAC and HAC. The acetate, butyrate and propionate level of cecal chyme were reduced in HAC group (-21.67%; -19.82%; -30.81%; p < 0.05). Microbial diversity and burden of Firmicutes were significantly decreased, while that of pathogenic bacteria, such as Bacteroidetes, Clostridium and Proteobacteria were increased in ammonia treated groups. Spearman’s correlation confirmed that burden of Ruminococcaceae_NK4A214_group showed significantly negative correlation with acetic acid (r = -0.67; p < 0.001) while Barnesiellaceae_unclassified showed significantly positive correlation with propionic acid (r = 0.50; p < 0.001). In conclusion, ammonia treatment was responsible for an imbalance of intestinal flora, which affected lipid metabolism and damaged intestinal barrier of rabbits, resulting in low growth performance due to lipid metabolism dysfunction. Public Library of Science 2021-06-30 /pmc/articles/PMC8244895/ /pubmed/34191811 http://dx.doi.org/10.1371/journal.pone.0252065 Text en © 2021 Cui et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cui, Jia
Yang, Xinyu
Wang, Fengxia
Liu, Shudong
Han, Shuaijuan
Chen, Baojiang
Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits
title Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits
title_full Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits
title_fullStr Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits
title_full_unstemmed Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits
title_short Effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits
title_sort effects of ammonia on growth performance, lipid metabolism and cecal microbial community of rabbits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8244895/
https://www.ncbi.nlm.nih.gov/pubmed/34191811
http://dx.doi.org/10.1371/journal.pone.0252065
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