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The complete chloroplast genome of Aconitum scaposum

Aconitum scaposum Franch 1894 belongs to the Genus Aconitum and Subgenus Lycoctonum (Ranunculaceae). It is widely distributed in China and adjacent areas, used as herbal medicine and had highy toxic components. This species has little reasearch information, especially its chloroplast (cp) genome inf...

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Autores principales: Zhang, Min, Luo, Jiawei, Su, Lijun, Ding, Qiaojiao, Yin, Xianmei, Hou, Feixia, Gao, Jihai, Peng, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245100/
https://www.ncbi.nlm.nih.gov/pubmed/34250240
http://dx.doi.org/10.1080/23802359.2021.1944380
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author Zhang, Min
Luo, Jiawei
Su, Lijun
Ding, Qiaojiao
Yin, Xianmei
Hou, Feixia
Gao, Jihai
Peng, Cheng
author_facet Zhang, Min
Luo, Jiawei
Su, Lijun
Ding, Qiaojiao
Yin, Xianmei
Hou, Feixia
Gao, Jihai
Peng, Cheng
author_sort Zhang, Min
collection PubMed
description Aconitum scaposum Franch 1894 belongs to the Genus Aconitum and Subgenus Lycoctonum (Ranunculaceae). It is widely distributed in China and adjacent areas, used as herbal medicine and had highy toxic components. This species has little reasearch information, especially its chloroplast (cp) genome information being unclear. Therefore, with the method of high salt and low pH to extract the cp of A. scaposum, we sequenced and assembled the complete cp genome of A. scaposum using Illumina high-throughput sequencing platform. The results showed the cp genome of A. scaposum was 157 688 bp in length, including a pair of inverted repeated regions (IRa 26 156 bp and IRb 26 232 bp, respectively), large single copy region (LSC 69 309 bp) and small single copy region (SSC 16 917 bp). And cp genome of A. scaposum consisted of 145 unique genes, 8 ribosomal RNA (rRNA) genes and 38 transfer RNA (tRNA) genes, with GC content was 38%. Meanwhile, based on the cp complete genome, we performed the phylogenetic tree of 66 species with maximum likelihood (ML) method, respectively. Among them, we selected one Delphinium species as the outgroup and the bootstrap of each braches were greater than 90%. The results indicated that the phylogenetic relationship of A. scaposum was relatively closely related to A. scaposum var. vaginatum compared to other Aconitum species.
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spelling pubmed-82451002021-07-09 The complete chloroplast genome of Aconitum scaposum Zhang, Min Luo, Jiawei Su, Lijun Ding, Qiaojiao Yin, Xianmei Hou, Feixia Gao, Jihai Peng, Cheng Mitochondrial DNA B Resour Mitogenome Announcement Aconitum scaposum Franch 1894 belongs to the Genus Aconitum and Subgenus Lycoctonum (Ranunculaceae). It is widely distributed in China and adjacent areas, used as herbal medicine and had highy toxic components. This species has little reasearch information, especially its chloroplast (cp) genome information being unclear. Therefore, with the method of high salt and low pH to extract the cp of A. scaposum, we sequenced and assembled the complete cp genome of A. scaposum using Illumina high-throughput sequencing platform. The results showed the cp genome of A. scaposum was 157 688 bp in length, including a pair of inverted repeated regions (IRa 26 156 bp and IRb 26 232 bp, respectively), large single copy region (LSC 69 309 bp) and small single copy region (SSC 16 917 bp). And cp genome of A. scaposum consisted of 145 unique genes, 8 ribosomal RNA (rRNA) genes and 38 transfer RNA (tRNA) genes, with GC content was 38%. Meanwhile, based on the cp complete genome, we performed the phylogenetic tree of 66 species with maximum likelihood (ML) method, respectively. Among them, we selected one Delphinium species as the outgroup and the bootstrap of each braches were greater than 90%. The results indicated that the phylogenetic relationship of A. scaposum was relatively closely related to A. scaposum var. vaginatum compared to other Aconitum species. Taylor & Francis 2021-06-28 /pmc/articles/PMC8245100/ /pubmed/34250240 http://dx.doi.org/10.1080/23802359.2021.1944380 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mitogenome Announcement
Zhang, Min
Luo, Jiawei
Su, Lijun
Ding, Qiaojiao
Yin, Xianmei
Hou, Feixia
Gao, Jihai
Peng, Cheng
The complete chloroplast genome of Aconitum scaposum
title The complete chloroplast genome of Aconitum scaposum
title_full The complete chloroplast genome of Aconitum scaposum
title_fullStr The complete chloroplast genome of Aconitum scaposum
title_full_unstemmed The complete chloroplast genome of Aconitum scaposum
title_short The complete chloroplast genome of Aconitum scaposum
title_sort complete chloroplast genome of aconitum scaposum
topic Mitogenome Announcement
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245100/
https://www.ncbi.nlm.nih.gov/pubmed/34250240
http://dx.doi.org/10.1080/23802359.2021.1944380
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