Cargando…

Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain

With global demand for SARS-CoV-2 testing ever rising, shortages in commercially available viral transport media pose a serious problem for laboratories and health care providers. For reliable diagnosis of SARS-CoV-2 and other respiratory viruses, executed by Real-time PCR, the quality of respirator...

Descripción completa

Detalles Bibliográficos
Autores principales:	Summer, Sabrina, Schmidt, Ralf, Herdina, Anna Nele, Krickl, Isabella, Madner, Julia, Greiner, Georg, Mayer, Florian J., Perkmann-Nagele, Nicole, Strassl, Robert
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245405/ https://www.ncbi.nlm.nih.gov/pubmed/34193912 http://dx.doi.org/10.1038/s41598-021-93028-8

Ejemplares similares

Performance Evaluation of the Fully Automated NeuMoDx RT-PCR Platform for the Quantification of CMV and EBV DNA in EDTA Plasma: Implications for Clinical Management and Establishment of a Conversion Formula
por: Herdina, Anna Nele, et al.
Publicado: (2022)

Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed
por: Mayer, Florian J., et al.
Publicado: (2020)

Analysis of the specificity of a COVID-19 antigen test in the Slovak mass testing program
por: Hledík, Michal, et al.
Publicado: (2021)

Surveillance of respiratory syncytial virus infections in adults, Austria, 2017 to 2019
por: Schubert, Lorenz, et al.
Publicado: (2021)

Diagnostic accuracy of nasopharyngeal swab, nasal swab and saliva swab samples for the detection of SARS-CoV-2 using RT-PCR
por: Tsujimoto, Yoshie, et al.
Publicado: (2021)

Female Genital Variation Far Exceeds That of Male Genitalia: A Review of Comparative Anatomy of Clitoris and the Female Lower Reproductive Tract in Theria
por: Pavlicev, Mihaela, et al.
Publicado: (2022)

Identification of Rickettsial Infections by Using Cutaneous Swab Specimens and PCR
por: Bechah, Yassina, et al.
Publicado: (2011)

Dacron swab and PBS are acceptable alternatives to flocked swab and viral transport media for SARS-CoV-2
por: Kline, Ahnika, et al.
Publicado: (2021)

Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM
por: Alves-Martin, Maria Fernanda, et al.
Publicado: (2023)

Comparison of nasal swabs with throat swabs for the detection of respiratory viruses by real-time reverse transcriptase PCR in adult Hajj pilgrims
por: Benkouiten, Samir, et al.
Publicado: (2015)

Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR
por: Preziuso, Silvia, et al.
Publicado: (2010)

Enhanced detection of Taylorella equigenitalis by qPCR using ‘Dry’ swabs
por: MAWHINNEY, Ian, et al.
Publicado: (2023)

Detection of SARS-CoV-2 RNA by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) on Self-Collected Nasal Swab Compared With Professionally Collected Nasopharyngeal Swab
por: Mannan, Nusrat, et al.
Publicado: (2022)

Efficiency of noninvasive sampling methods (swab) together with Polymerase Chain Reaction (PCR) for diagnosing American Tegumentary Leishmaniasis
por: Boni, Sara Macente, et al.
Publicado: (2017)

Detection of Rickettsia sibirica mongolitimonae by Using Cutaneous Swab Samples and Quantitative PCR
por: Solary, Julie, et al.
Publicado: (2014)

Detection of Pneumocystis in the nasal swabs of immune-suppressed rats by use of PCR and microscopy
por: Can, Hüseyin, et al.
Publicado: (2013)

Optimising the assignment of swabs and reagent for PCR testing during a viral epidemic()
por: Santini, Alberto
Publicado: (2021)

Hypertensive Crisis in Pregnancy with COVID19: Confirmed with rt-PCR for Nasopharyngeal Swab
por: Bandara, Sajith, et al.
Publicado: (2020)

A 3D-printed nasopharyngeal swab for COVID-19 diagnostic testing
por: Ford, Jonathan, et al.
Publicado: (2020)

Diagnosis of Queensland Tick Typhus and African Tick Bite Fever by PCR of Lesion Swabs
por: Wang, Jin-Mei, et al.
Publicado: (2009)

A cost effective real-time PCR for the detection of adenovirus from viral swabs
por: Al-Siyabi, Turkiya, et al.
Publicado: (2013)

Pooling skin swabs does not inhibit qPCR detection of amphibian chytrid infection
por: Sabino-Pinto, Joana, et al.
Publicado: (2019)

The value of anal swab RT-PCR for COVID-19 diagnosis in adult Indonesian patients
por: Abdullah, Murdani, et al.
Publicado: (2021)

SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
por: Guimarães, Taísa Coelho, et al.
Publicado: (2022)

Sampling, Logistics, and Analytics of Urine for RT-qPCR-based Diagnostics
por: Kretschmer-Kazemi Far, Rosel, et al.
Publicado: (2021)

Sensory and analytical characterization of the “cool‐melting” perception of commercial spreads
por: Galindo‐Cuspinera, Verónica, et al.
Publicado: (2017)

A cross-sectional assessment of PRRSV nucleic acid detection by RT-qPCR in serum, ear-vein blood swabs, nasal swabs, and oral swabs from weaning-age pigs under field conditions
por: Osemeke, Onyekachukwu H., et al.
Publicado: (2023)

Serum antibody response to BNT162b2 after natural SARS‐CoV‐2 infection
por: Perkmann, Thomas, et al.
Publicado: (2021)

Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
por: de Souza Figueiredo, Eduardo Eustáquio, et al.
Publicado: (2010)

Evaluation of a Chlamydia trachomatis-specific, commercial, real-time PCR for use with ocular swabs
por: Pickering, Harry, et al.
Publicado: (2018)

Reduced sensitivity of SARS-CoV-2 PCR testing with single-nostril nasal swabs
por: Salvatore, Phillip P., et al.
Publicado: (2021)

Conjunctival Swab Real Time-PCR in Leishmania infantum Seropositive Dogs: Diagnostic and Prognostic Values
por: Cavalera, Maria Alfonsa, et al.
Publicado: (2022)

Comparative Evaluation of Three Chromogenic Media Combined with Broth Enrichment and the Real-Time PCR-Based Xpert MRSA Assay for Screening of Methicillin-Resistant Staphylococcus aureus in Nasal Swabs
por: Lee, Seungok, et al.
Publicado: (2013)

Anti-Spike Protein Assays to Determine SARS-CoV-2 Antibody Levels: a Head-to-Head Comparison of Five Quantitative Assays
por: Perkmann, Thomas, et al.
Publicado: (2021)

Initial SARS-CoV-2 vaccination response can predict booster response for BNT162b2 but not for AZD1222
por: Perkmann, Thomas, et al.
Publicado: (2021)

Swabbing Thoroughly People for COVID-19 Positivity. Insights on the Main Bio-analytical and Microbiology Bias and Concerns
por: Chirumbolo, Salvatore
Publicado: (2020)

Quantification of copepod gut content by differential length amplification quantitative PCR (dla-qPCR)
por: Troedsson, Christofer, et al.
Publicado: (2009)

Factors influencing the contamination rates of the conjunctival swabs and organ culture media of human donor eyes
por: Röck, Tobias, et al.
Publicado: (2018)

Approach to Assessment of New Swabs and Viral Transport Media for SARS-CoV-2 Testing
por: Locher, Kerstin, et al.
Publicado: (2021)

How to forget a “traumatic” experience: a case report of transient global amnesia after nasopharyngeal swab for Coronavirus disease 19
por: Ravaglia, Sabrina, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_b0i9bt244acrf8trkqm6k8mket