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VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine
Resveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accessi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245564/ https://www.ncbi.nlm.nih.gov/pubmed/34193849 http://dx.doi.org/10.1038/s41438-021-00585-0 |
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author | Jiang, Changyue Wang, Dan Zhang, Jie Xu, Yan Zhang, Chaohong Zhang, Jianxia Wang, Xiping Wang, Yuejin |
author_facet | Jiang, Changyue Wang, Dan Zhang, Jie Xu, Yan Zhang, Chaohong Zhang, Jianxia Wang, Xiping Wang, Yuejin |
author_sort | Jiang, Changyue |
collection | PubMed |
description | Resveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accession Danfeng-2 is strongly expressed under artificial inoculation with Uncinula necator and regulates resveratrol accumulation. Unlike its homolog, VqMYB154 has a pathogen-induced promoter and responds to stimulation by U. necator, Pseudomonas syringae, and other treatments. Yeast one-hybrid and GUS activity assays confirmed that VqMYB154 can activate the stilbene synthase genes VqSTS9, VqSTS32, and VqSTS42 by directly binding to their promoters. Overexpression of VqMYB154 in grape leaves resulted in activation of the stilbene pathway, upregulation of STS genes, and accumulation of stilbenoids. In addition, heterologous overexpression of VqMYB154 in Arabidopsis activated resistance-related genes and resulted in greater programmed cell death and accumulation of reactive oxygen species, which led to resistance against P. syringae. These results suggest that the transcription factor VqMYB154 from V. quinquangularis accession Danfeng-2 participates in the regulatory mechanism that improves the biosynthesis and accumulation of stilbenes and enhances resistance to disease in grapevine. |
format | Online Article Text |
id | pubmed-8245564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82455642021-07-20 VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine Jiang, Changyue Wang, Dan Zhang, Jie Xu, Yan Zhang, Chaohong Zhang, Jianxia Wang, Xiping Wang, Yuejin Hortic Res Article Resveratrol plays a crucial phytoalexin role in the grapevine and is beneficial to human health. However, the molecular mechanism of resveratrol accumulation in the enhancement of disease resistance is unclear. Here, we report that the transcription factor VqMYB154 from Vitis quinquangularis accession Danfeng-2 is strongly expressed under artificial inoculation with Uncinula necator and regulates resveratrol accumulation. Unlike its homolog, VqMYB154 has a pathogen-induced promoter and responds to stimulation by U. necator, Pseudomonas syringae, and other treatments. Yeast one-hybrid and GUS activity assays confirmed that VqMYB154 can activate the stilbene synthase genes VqSTS9, VqSTS32, and VqSTS42 by directly binding to their promoters. Overexpression of VqMYB154 in grape leaves resulted in activation of the stilbene pathway, upregulation of STS genes, and accumulation of stilbenoids. In addition, heterologous overexpression of VqMYB154 in Arabidopsis activated resistance-related genes and resulted in greater programmed cell death and accumulation of reactive oxygen species, which led to resistance against P. syringae. These results suggest that the transcription factor VqMYB154 from V. quinquangularis accession Danfeng-2 participates in the regulatory mechanism that improves the biosynthesis and accumulation of stilbenes and enhances resistance to disease in grapevine. Nature Publishing Group UK 2021-07-01 /pmc/articles/PMC8245564/ /pubmed/34193849 http://dx.doi.org/10.1038/s41438-021-00585-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Jiang, Changyue Wang, Dan Zhang, Jie Xu, Yan Zhang, Chaohong Zhang, Jianxia Wang, Xiping Wang, Yuejin VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine |
title | VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine |
title_full | VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine |
title_fullStr | VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine |
title_full_unstemmed | VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine |
title_short | VqMYB154 promotes polygene expression and enhances resistance to pathogens in Chinese wild grapevine |
title_sort | vqmyb154 promotes polygene expression and enhances resistance to pathogens in chinese wild grapevine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245564/ https://www.ncbi.nlm.nih.gov/pubmed/34193849 http://dx.doi.org/10.1038/s41438-021-00585-0 |
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