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Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors
Xyloglucans are highly substituted and recalcitrant polysaccharides found in the primary cell walls of vascular plants, acting as a barrier against pathogens. Here, we reveal that the diverse and economically relevant Xanthomonas bacteria are endowed with a xyloglucan depolymerization machinery that...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245568/ https://www.ncbi.nlm.nih.gov/pubmed/34193873 http://dx.doi.org/10.1038/s41467-021-24277-4 |
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author | Vieira, Plinio S. Bonfim, Isabela M. Araujo, Evandro A. Melo, Ricardo R. Lima, Augusto R. Fessel, Melissa R. Paixão, Douglas A. A. Persinoti, Gabriela F. Rocco, Silvana A. Lima, Tatiani B. Pirolla, Renan A. S. Morais, Mariana A. B. Correa, Jessica B. L. Zanphorlin, Leticia M. Diogo, Jose A. Lima, Evandro A. Grandis, Adriana Buckeridge, Marcos S. Gozzo, Fabio C. Benedetti, Celso E. Polikarpov, Igor Giuseppe, Priscila O. Murakami, Mario T. |
author_facet | Vieira, Plinio S. Bonfim, Isabela M. Araujo, Evandro A. Melo, Ricardo R. Lima, Augusto R. Fessel, Melissa R. Paixão, Douglas A. A. Persinoti, Gabriela F. Rocco, Silvana A. Lima, Tatiani B. Pirolla, Renan A. S. Morais, Mariana A. B. Correa, Jessica B. L. Zanphorlin, Leticia M. Diogo, Jose A. Lima, Evandro A. Grandis, Adriana Buckeridge, Marcos S. Gozzo, Fabio C. Benedetti, Celso E. Polikarpov, Igor Giuseppe, Priscila O. Murakami, Mario T. |
author_sort | Vieira, Plinio S. |
collection | PubMed |
description | Xyloglucans are highly substituted and recalcitrant polysaccharides found in the primary cell walls of vascular plants, acting as a barrier against pathogens. Here, we reveal that the diverse and economically relevant Xanthomonas bacteria are endowed with a xyloglucan depolymerization machinery that is linked to pathogenesis. Using the citrus canker pathogen as a model organism, we show that this system encompasses distinctive glycoside hydrolases, a modular xyloglucan acetylesterase and specific membrane transporters, demonstrating that plant-associated bacteria employ distinct molecular strategies from commensal gut bacteria to cope with xyloglucans. Notably, the sugars released by this system elicit the expression of several key virulence factors, including the type III secretion system, a membrane-embedded apparatus to deliver effector proteins into the host cells. Together, these findings shed light on the molecular mechanisms underpinning the intricate enzymatic machinery of Xanthomonas to depolymerize xyloglucans and uncover a role for this system in signaling pathways driving pathogenesis. |
format | Online Article Text |
id | pubmed-8245568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82455682021-07-20 Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors Vieira, Plinio S. Bonfim, Isabela M. Araujo, Evandro A. Melo, Ricardo R. Lima, Augusto R. Fessel, Melissa R. Paixão, Douglas A. A. Persinoti, Gabriela F. Rocco, Silvana A. Lima, Tatiani B. Pirolla, Renan A. S. Morais, Mariana A. B. Correa, Jessica B. L. Zanphorlin, Leticia M. Diogo, Jose A. Lima, Evandro A. Grandis, Adriana Buckeridge, Marcos S. Gozzo, Fabio C. Benedetti, Celso E. Polikarpov, Igor Giuseppe, Priscila O. Murakami, Mario T. Nat Commun Article Xyloglucans are highly substituted and recalcitrant polysaccharides found in the primary cell walls of vascular plants, acting as a barrier against pathogens. Here, we reveal that the diverse and economically relevant Xanthomonas bacteria are endowed with a xyloglucan depolymerization machinery that is linked to pathogenesis. Using the citrus canker pathogen as a model organism, we show that this system encompasses distinctive glycoside hydrolases, a modular xyloglucan acetylesterase and specific membrane transporters, demonstrating that plant-associated bacteria employ distinct molecular strategies from commensal gut bacteria to cope with xyloglucans. Notably, the sugars released by this system elicit the expression of several key virulence factors, including the type III secretion system, a membrane-embedded apparatus to deliver effector proteins into the host cells. Together, these findings shed light on the molecular mechanisms underpinning the intricate enzymatic machinery of Xanthomonas to depolymerize xyloglucans and uncover a role for this system in signaling pathways driving pathogenesis. Nature Publishing Group UK 2021-06-30 /pmc/articles/PMC8245568/ /pubmed/34193873 http://dx.doi.org/10.1038/s41467-021-24277-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Vieira, Plinio S. Bonfim, Isabela M. Araujo, Evandro A. Melo, Ricardo R. Lima, Augusto R. Fessel, Melissa R. Paixão, Douglas A. A. Persinoti, Gabriela F. Rocco, Silvana A. Lima, Tatiani B. Pirolla, Renan A. S. Morais, Mariana A. B. Correa, Jessica B. L. Zanphorlin, Leticia M. Diogo, Jose A. Lima, Evandro A. Grandis, Adriana Buckeridge, Marcos S. Gozzo, Fabio C. Benedetti, Celso E. Polikarpov, Igor Giuseppe, Priscila O. Murakami, Mario T. Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors |
title | Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors |
title_full | Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors |
title_fullStr | Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors |
title_full_unstemmed | Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors |
title_short | Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors |
title_sort | xyloglucan processing machinery in xanthomonas pathogens and its role in the transcriptional activation of virulence factors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245568/ https://www.ncbi.nlm.nih.gov/pubmed/34193873 http://dx.doi.org/10.1038/s41467-021-24277-4 |
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