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Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality

Horticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for ass...

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Autores principales: Mason, Tyler J., Bettenhausen, Harmonie M., Chaparro, Jacqueline M., Uchanski, Mark E., Prenni, Jessica E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245583/
https://www.ncbi.nlm.nih.gov/pubmed/34193823
http://dx.doi.org/10.1038/s41438-021-00596-x
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author Mason, Tyler J.
Bettenhausen, Harmonie M.
Chaparro, Jacqueline M.
Uchanski, Mark E.
Prenni, Jessica E.
author_facet Mason, Tyler J.
Bettenhausen, Harmonie M.
Chaparro, Jacqueline M.
Uchanski, Mark E.
Prenni, Jessica E.
author_sort Mason, Tyler J.
collection PubMed
description Horticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess quality characteristics in various pepper (Capsicum annuum L.) cultivars. DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints, while REIMS could distinguish pepper market class (e.g., bell, lunchbox, and popper). Furthermore, DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C, p-coumaric acid, and capsaicin. The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality.
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spelling pubmed-82455832021-07-20 Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality Mason, Tyler J. Bettenhausen, Harmonie M. Chaparro, Jacqueline M. Uchanski, Mark E. Prenni, Jessica E. Hortic Res Method Horticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess quality characteristics in various pepper (Capsicum annuum L.) cultivars. DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints, while REIMS could distinguish pepper market class (e.g., bell, lunchbox, and popper). Furthermore, DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C, p-coumaric acid, and capsaicin. The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality. Nature Publishing Group UK 2021-07-01 /pmc/articles/PMC8245583/ /pubmed/34193823 http://dx.doi.org/10.1038/s41438-021-00596-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Method
Mason, Tyler J.
Bettenhausen, Harmonie M.
Chaparro, Jacqueline M.
Uchanski, Mark E.
Prenni, Jessica E.
Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
title Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
title_full Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
title_fullStr Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
title_full_unstemmed Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
title_short Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
title_sort evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245583/
https://www.ncbi.nlm.nih.gov/pubmed/34193823
http://dx.doi.org/10.1038/s41438-021-00596-x
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