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The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2

Jasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senesc...

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Autores principales: Yang, Kuo, An, Jian-Ping, Li, Chong-Yang, Shen, Xue-Na, Liu, Ya-Jing, Wang, Da-Ru, Ji, Xing-Long, Hao, Yu-Jin, You, Chun-Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245655/
https://www.ncbi.nlm.nih.gov/pubmed/34193837
http://dx.doi.org/10.1038/s41438-021-00593-0
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author Yang, Kuo
An, Jian-Ping
Li, Chong-Yang
Shen, Xue-Na
Liu, Ya-Jing
Wang, Da-Ru
Ji, Xing-Long
Hao, Yu-Jin
You, Chun-Xiang
author_facet Yang, Kuo
An, Jian-Ping
Li, Chong-Yang
Shen, Xue-Na
Liu, Ya-Jing
Wang, Da-Ru
Ji, Xing-Long
Hao, Yu-Jin
You, Chun-Xiang
author_sort Yang, Kuo
collection PubMed
description Jasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.
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spelling pubmed-82456552021-07-20 The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2 Yang, Kuo An, Jian-Ping Li, Chong-Yang Shen, Xue-Na Liu, Ya-Jing Wang, Da-Ru Ji, Xing-Long Hao, Yu-Jin You, Chun-Xiang Hortic Res Article Jasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple. Nature Publishing Group UK 2021-07-01 /pmc/articles/PMC8245655/ /pubmed/34193837 http://dx.doi.org/10.1038/s41438-021-00593-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yang, Kuo
An, Jian-Ping
Li, Chong-Yang
Shen, Xue-Na
Liu, Ya-Jing
Wang, Da-Ru
Ji, Xing-Long
Hao, Yu-Jin
You, Chun-Xiang
The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2
title The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2
title_full The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2
title_fullStr The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2
title_full_unstemmed The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2
title_short The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2
title_sort apple c2h2-type zinc finger transcription factor mdzat10 positively regulates ja-induced leaf senescence by interacting with mdbt2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245655/
https://www.ncbi.nlm.nih.gov/pubmed/34193837
http://dx.doi.org/10.1038/s41438-021-00593-0
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