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The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene

To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single Saccharomyces cerevisiae beta-tubulin gene. However, we...

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Detalles Bibliográficos
Autores principales: Gibson, Sophia B, Harper, Clare S, Lackner, Laura L, Andersen, Erik C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246273/
https://www.ncbi.nlm.nih.gov/pubmed/34222836
http://dx.doi.org/10.17912/micropub.biology.000411
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author Gibson, Sophia B
Harper, Clare S
Lackner, Laura L
Andersen, Erik C
author_facet Gibson, Sophia B
Harper, Clare S
Lackner, Laura L
Andersen, Erik C
author_sort Gibson, Sophia B
collection PubMed
description To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single Saccharomyces cerevisiae beta-tubulin gene. However, we found that haploid yeast cells containing nematode-specific beta-tubulin genes were not viable, suggesting that nematode beta-tubulin cannot substitute for the loss of the yeast beta-tubulin gene.
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spelling pubmed-82462732021-07-01 The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene Gibson, Sophia B Harper, Clare S Lackner, Laura L Andersen, Erik C MicroPubl Biol New Finding To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single Saccharomyces cerevisiae beta-tubulin gene. However, we found that haploid yeast cells containing nematode-specific beta-tubulin genes were not viable, suggesting that nematode beta-tubulin cannot substitute for the loss of the yeast beta-tubulin gene. Caltech Library 2021-06-30 /pmc/articles/PMC8246273/ /pubmed/34222836 http://dx.doi.org/10.17912/micropub.biology.000411 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Gibson, Sophia B
Harper, Clare S
Lackner, Laura L
Andersen, Erik C
The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene
title The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene
title_full The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene
title_fullStr The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene
title_full_unstemmed The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene
title_short The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene
title_sort caenorhabditis elegans and haemonchus contortus beta-tubulin genes cannot substitute for loss of the saccharomyces cerevisiae beta-tubulin gene
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246273/
https://www.ncbi.nlm.nih.gov/pubmed/34222836
http://dx.doi.org/10.17912/micropub.biology.000411
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