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The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene
To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single Saccharomyces cerevisiae beta-tubulin gene. However, we...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246273/ https://www.ncbi.nlm.nih.gov/pubmed/34222836 http://dx.doi.org/10.17912/micropub.biology.000411 |
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author | Gibson, Sophia B Harper, Clare S Lackner, Laura L Andersen, Erik C |
author_facet | Gibson, Sophia B Harper, Clare S Lackner, Laura L Andersen, Erik C |
author_sort | Gibson, Sophia B |
collection | PubMed |
description | To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single Saccharomyces cerevisiae beta-tubulin gene. However, we found that haploid yeast cells containing nematode-specific beta-tubulin genes were not viable, suggesting that nematode beta-tubulin cannot substitute for the loss of the yeast beta-tubulin gene. |
format | Online Article Text |
id | pubmed-8246273 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-82462732021-07-01 The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene Gibson, Sophia B Harper, Clare S Lackner, Laura L Andersen, Erik C MicroPubl Biol New Finding To better understand the mechanism of resistance caused by putative interactions between beta-tubulin and benzimidazole compounds, we sought to purify nematode-specific beta-tubulins using heterologous expression after replacement of the single Saccharomyces cerevisiae beta-tubulin gene. However, we found that haploid yeast cells containing nematode-specific beta-tubulin genes were not viable, suggesting that nematode beta-tubulin cannot substitute for the loss of the yeast beta-tubulin gene. Caltech Library 2021-06-30 /pmc/articles/PMC8246273/ /pubmed/34222836 http://dx.doi.org/10.17912/micropub.biology.000411 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Gibson, Sophia B Harper, Clare S Lackner, Laura L Andersen, Erik C The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene |
title | The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene |
title_full | The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene |
title_fullStr | The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene |
title_full_unstemmed | The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene |
title_short | The Caenorhabditis elegans and Haemonchus contortus beta-tubulin genes cannot substitute for loss of the Saccharomyces cerevisiae beta-tubulin gene |
title_sort | caenorhabditis elegans and haemonchus contortus beta-tubulin genes cannot substitute for loss of the saccharomyces cerevisiae beta-tubulin gene |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246273/ https://www.ncbi.nlm.nih.gov/pubmed/34222836 http://dx.doi.org/10.17912/micropub.biology.000411 |
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