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Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by...

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Autores principales: Kim, Do-Hyeon, Chang, Yeonho, Park, Soyeon, Jeong, Min Gyu, Kwon, Yonghoon, Zhou, Kai, Noh, Jungeun, Choi, Yun-Kyu, Hong, Triet Minh, Chang, Young-Tae, Ryu, Sung Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246296/
https://www.ncbi.nlm.nih.gov/pubmed/34257864
http://dx.doi.org/10.1039/d1sc00612f
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author Kim, Do-Hyeon
Chang, Yeonho
Park, Soyeon
Jeong, Min Gyu
Kwon, Yonghoon
Zhou, Kai
Noh, Jungeun
Choi, Yun-Kyu
Hong, Triet Minh
Chang, Young-Tae
Ryu, Sung Ho
author_facet Kim, Do-Hyeon
Chang, Yeonho
Park, Soyeon
Jeong, Min Gyu
Kwon, Yonghoon
Zhou, Kai
Noh, Jungeun
Choi, Yun-Kyu
Hong, Triet Minh
Chang, Young-Tae
Ryu, Sung Ho
author_sort Kim, Do-Hyeon
collection PubMed
description Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion.
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spelling pubmed-82462962021-07-12 Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging Kim, Do-Hyeon Chang, Yeonho Park, Soyeon Jeong, Min Gyu Kwon, Yonghoon Zhou, Kai Noh, Jungeun Choi, Yun-Kyu Hong, Triet Minh Chang, Young-Tae Ryu, Sung Ho Chem Sci Chemistry Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion. The Royal Society of Chemistry 2021-05-18 /pmc/articles/PMC8246296/ /pubmed/34257864 http://dx.doi.org/10.1039/d1sc00612f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Kim, Do-Hyeon
Chang, Yeonho
Park, Soyeon
Jeong, Min Gyu
Kwon, Yonghoon
Zhou, Kai
Noh, Jungeun
Choi, Yun-Kyu
Hong, Triet Minh
Chang, Young-Tae
Ryu, Sung Ho
Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
title Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
title_full Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
title_fullStr Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
title_full_unstemmed Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
title_short Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
title_sort blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246296/
https://www.ncbi.nlm.nih.gov/pubmed/34257864
http://dx.doi.org/10.1039/d1sc00612f
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