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Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging
Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246296/ https://www.ncbi.nlm.nih.gov/pubmed/34257864 http://dx.doi.org/10.1039/d1sc00612f |
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author | Kim, Do-Hyeon Chang, Yeonho Park, Soyeon Jeong, Min Gyu Kwon, Yonghoon Zhou, Kai Noh, Jungeun Choi, Yun-Kyu Hong, Triet Minh Chang, Young-Tae Ryu, Sung Ho |
author_facet | Kim, Do-Hyeon Chang, Yeonho Park, Soyeon Jeong, Min Gyu Kwon, Yonghoon Zhou, Kai Noh, Jungeun Choi, Yun-Kyu Hong, Triet Minh Chang, Young-Tae Ryu, Sung Ho |
author_sort | Kim, Do-Hyeon |
collection | PubMed |
description | Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion. |
format | Online Article Text |
id | pubmed-8246296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-82462962021-07-12 Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging Kim, Do-Hyeon Chang, Yeonho Park, Soyeon Jeong, Min Gyu Kwon, Yonghoon Zhou, Kai Noh, Jungeun Choi, Yun-Kyu Hong, Triet Minh Chang, Young-Tae Ryu, Sung Ho Chem Sci Chemistry Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by invading predefined spectral windows, as demonstrated in the case study using EGFR and Tensin2. These multicolor image artifacts become much critical in localization-based superresolution microscopy as the blue-converted dyes are photoactivatable. We provide a practical guideline for the use of organic dyes for multicolor imaging to prevent artifacts derived by blue-conversion. The Royal Society of Chemistry 2021-05-18 /pmc/articles/PMC8246296/ /pubmed/34257864 http://dx.doi.org/10.1039/d1sc00612f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Kim, Do-Hyeon Chang, Yeonho Park, Soyeon Jeong, Min Gyu Kwon, Yonghoon Zhou, Kai Noh, Jungeun Choi, Yun-Kyu Hong, Triet Minh Chang, Young-Tae Ryu, Sung Ho Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging |
title | Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging |
title_full | Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging |
title_fullStr | Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging |
title_full_unstemmed | Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging |
title_short | Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging |
title_sort | blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246296/ https://www.ncbi.nlm.nih.gov/pubmed/34257864 http://dx.doi.org/10.1039/d1sc00612f |
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