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Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)

A combination of direct liquid extraction using a small volume of solvent and electrospray ionization allows the rapid measurement of complex chemical components in biological samples and visualization of their distribution in tissue sections. This review describes the development of such techniques...

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Autor principal: Otsuka, Yoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Mass Spectrometry Society of Japan 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246329/
https://www.ncbi.nlm.nih.gov/pubmed/34249586
http://dx.doi.org/10.5702/massspectrometry.A0095
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author Otsuka, Yoichi
author_facet Otsuka, Yoichi
author_sort Otsuka, Yoichi
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description A combination of direct liquid extraction using a small volume of solvent and electrospray ionization allows the rapid measurement of complex chemical components in biological samples and visualization of their distribution in tissue sections. This review describes the development of such techniques and their application to biological research since the first reports in the early 2000s. An overview of electrospray ionization, ion suppression in samples, and the acceleration of specific chemical reactions in charged droplets is also presented. Potential future applications for visualizing multimolecular environments in biological systems are discussed.
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spelling pubmed-82463292021-07-08 Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) Otsuka, Yoichi Mass Spectrom (Tokyo) Review A combination of direct liquid extraction using a small volume of solvent and electrospray ionization allows the rapid measurement of complex chemical components in biological samples and visualization of their distribution in tissue sections. This review describes the development of such techniques and their application to biological research since the first reports in the early 2000s. An overview of electrospray ionization, ion suppression in samples, and the acceleration of specific chemical reactions in charged droplets is also presented. Potential future applications for visualizing multimolecular environments in biological systems are discussed. The Mass Spectrometry Society of Japan 2021 2021-06-30 /pmc/articles/PMC8246329/ /pubmed/34249586 http://dx.doi.org/10.5702/massspectrometry.A0095 Text en Copyright © 2021 Yoichi Otsuka. https://creativecommons.org/licenses/by/2.5/This is an open-access article distributed under the terms of Creative Commons Attribution Non-Commercial 4.0 International License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Review
Otsuka, Yoichi
Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)
title Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)
title_full Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)
title_fullStr Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)
title_full_unstemmed Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)
title_short Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)
title_sort direct liquid extraction and ionization techniques for understanding multimolecular environments in biological systems (secondary publication)
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246329/
https://www.ncbi.nlm.nih.gov/pubmed/34249586
http://dx.doi.org/10.5702/massspectrometry.A0095
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