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Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication)
A combination of direct liquid extraction using a small volume of solvent and electrospray ionization allows the rapid measurement of complex chemical components in biological samples and visualization of their distribution in tissue sections. This review describes the development of such techniques...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Mass Spectrometry Society of Japan
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246329/ https://www.ncbi.nlm.nih.gov/pubmed/34249586 http://dx.doi.org/10.5702/massspectrometry.A0095 |
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author | Otsuka, Yoichi |
author_facet | Otsuka, Yoichi |
author_sort | Otsuka, Yoichi |
collection | PubMed |
description | A combination of direct liquid extraction using a small volume of solvent and electrospray ionization allows the rapid measurement of complex chemical components in biological samples and visualization of their distribution in tissue sections. This review describes the development of such techniques and their application to biological research since the first reports in the early 2000s. An overview of electrospray ionization, ion suppression in samples, and the acceleration of specific chemical reactions in charged droplets is also presented. Potential future applications for visualizing multimolecular environments in biological systems are discussed. |
format | Online Article Text |
id | pubmed-8246329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Mass Spectrometry Society of Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-82463292021-07-08 Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) Otsuka, Yoichi Mass Spectrom (Tokyo) Review A combination of direct liquid extraction using a small volume of solvent and electrospray ionization allows the rapid measurement of complex chemical components in biological samples and visualization of their distribution in tissue sections. This review describes the development of such techniques and their application to biological research since the first reports in the early 2000s. An overview of electrospray ionization, ion suppression in samples, and the acceleration of specific chemical reactions in charged droplets is also presented. Potential future applications for visualizing multimolecular environments in biological systems are discussed. The Mass Spectrometry Society of Japan 2021 2021-06-30 /pmc/articles/PMC8246329/ /pubmed/34249586 http://dx.doi.org/10.5702/massspectrometry.A0095 Text en Copyright © 2021 Yoichi Otsuka. https://creativecommons.org/licenses/by/2.5/This is an open-access article distributed under the terms of Creative Commons Attribution Non-Commercial 4.0 International License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Review Otsuka, Yoichi Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) |
title | Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) |
title_full | Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) |
title_fullStr | Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) |
title_full_unstemmed | Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) |
title_short | Direct Liquid Extraction and Ionization Techniques for Understanding Multimolecular Environments in Biological Systems (Secondary Publication) |
title_sort | direct liquid extraction and ionization techniques for understanding multimolecular environments in biological systems (secondary publication) |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246329/ https://www.ncbi.nlm.nih.gov/pubmed/34249586 http://dx.doi.org/10.5702/massspectrometry.A0095 |
work_keys_str_mv | AT otsukayoichi directliquidextractionandionizationtechniquesforunderstandingmultimolecularenvironmentsinbiologicalsystemssecondarypublication |