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A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency
In rice (Oryza sativa), amylose content (AC) is the major factor that determines eating and cooking quality (ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy (Wx) locus. Here we identified a rare Wx allele, Wx (mw), which combines a favorable AC, improved ECQ...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246539/ https://www.ncbi.nlm.nih.gov/pubmed/32886440 http://dx.doi.org/10.1111/jipb.13010 |
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author | Zhang, Changquan Yang, Yong Chen, Shengjie Liu, Xueju Zhu, Jihui Zhou, Lihui Lu, Yan Li, Qianfeng Fan, Xiaolei Tang, Shuzhu Gu, Minghong Liu, Qiaoquan |
author_facet | Zhang, Changquan Yang, Yong Chen, Shengjie Liu, Xueju Zhu, Jihui Zhou, Lihui Lu, Yan Li, Qianfeng Fan, Xiaolei Tang, Shuzhu Gu, Minghong Liu, Qiaoquan |
author_sort | Zhang, Changquan |
collection | PubMed |
description | In rice (Oryza sativa), amylose content (AC) is the major factor that determines eating and cooking quality (ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy (Wx) locus. Here we identified a rare Wx allele, Wx (mw), which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx (mw) may have derived from recombination between two important natural Wx alleles, Wx (in) and Wx (b). We validated the effects of Wx (mw) on rice grain quality using both transgenic lines and near‐isogenic lines (NILs). When introgressed into the japonica Nipponbare (NIP) background, Wx (mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx (b) allele and NILs with the Wx (mp) allele. Notably, mature grains of NILs fixed for Wx (mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx (mw) into a high‐yielding japonica cultivar via molecular marker‐assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx (mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high‐yielding japonica cultivars, in rice breeding programs. |
format | Online Article Text |
id | pubmed-8246539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82465392021-07-02 A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency Zhang, Changquan Yang, Yong Chen, Shengjie Liu, Xueju Zhu, Jihui Zhou, Lihui Lu, Yan Li, Qianfeng Fan, Xiaolei Tang, Shuzhu Gu, Minghong Liu, Qiaoquan J Integr Plant Biol Molecular Physiology In rice (Oryza sativa), amylose content (AC) is the major factor that determines eating and cooking quality (ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy (Wx) locus. Here we identified a rare Wx allele, Wx (mw), which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx (mw) may have derived from recombination between two important natural Wx alleles, Wx (in) and Wx (b). We validated the effects of Wx (mw) on rice grain quality using both transgenic lines and near‐isogenic lines (NILs). When introgressed into the japonica Nipponbare (NIP) background, Wx (mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx (b) allele and NILs with the Wx (mp) allele. Notably, mature grains of NILs fixed for Wx (mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx (mw) into a high‐yielding japonica cultivar via molecular marker‐assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx (mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high‐yielding japonica cultivars, in rice breeding programs. John Wiley and Sons Inc. 2020-12-29 2021-05 /pmc/articles/PMC8246539/ /pubmed/32886440 http://dx.doi.org/10.1111/jipb.13010 Text en © 2020 The Authors. Journal of Integrative Plant Biology Published by John Wiley & Sons Australia, Ltd on behalf of Institute of Botany, Chinese Academy of Sciences https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Molecular Physiology Zhang, Changquan Yang, Yong Chen, Shengjie Liu, Xueju Zhu, Jihui Zhou, Lihui Lu, Yan Li, Qianfeng Fan, Xiaolei Tang, Shuzhu Gu, Minghong Liu, Qiaoquan A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency |
title | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency |
title_full | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency |
title_fullStr | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency |
title_full_unstemmed | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency |
title_short | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency |
title_sort | rare waxy allele coordinately improves rice eating and cooking quality and grain transparency |
topic | Molecular Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246539/ https://www.ncbi.nlm.nih.gov/pubmed/32886440 http://dx.doi.org/10.1111/jipb.13010 |
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