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Analysis of proteostasis during aging with western blot of detergent-soluble and insoluble protein fractions
Defects in protein quality control are the underlying cause of age-related diseases. The western blot analysis of detergent-soluble and insoluble protein fractions has proven useful in identifying interventions that regulate proteostasis. Here, we describe the protocol for such analyses in Drosophil...
Autores principales: | Rai, Mamta, Curley, Michelle, Coleman, Zane, Nityanandam, Anjana, Jiao, Jianqin, Graca, Flavia A., Hunt, Liam C., Demontis, Fabio |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246638/ https://www.ncbi.nlm.nih.gov/pubmed/34235493 http://dx.doi.org/10.1016/j.xpro.2021.100628 |
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