A protocol for human bone marrow adipocyte isolation and purification

Primary human bone marrow adipocytes (BM-Ads) display a specific metabolism that is not recapitulated by in vitro differentiated bone marrow mesenchymal stromal cells. These findings highlight the need for using primary BM-Ads in studies of the metabolic impact of BM-Ads on surrounding cells. Here,...

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Detalles Bibliográficos
Autores principales: Attané, Camille, Estève, David, Moutahir, Mohamed, Reina, Nicolas, Muller, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246640/
https://www.ncbi.nlm.nih.gov/pubmed/34235494
http://dx.doi.org/10.1016/j.xpro.2021.100629
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author Attané, Camille
Estève, David
Moutahir, Mohamed
Reina, Nicolas
Muller, Catherine
author_facet Attané, Camille
Estève, David
Moutahir, Mohamed
Reina, Nicolas
Muller, Catherine
author_sort Attané, Camille
collection PubMed
description Primary human bone marrow adipocytes (BM-Ads) display a specific metabolism that is not recapitulated by in vitro differentiated bone marrow mesenchymal stromal cells. These findings highlight the need for using primary BM-Ads in studies of the metabolic impact of BM-Ads on surrounding cells. Here, we present a protocol for isolating human BM-Ads from bone marrow aspirates and verifying adipocyte suspension purity. These isolated and purified BM-Ads can be used for functional assays or frozen for molecular analyses. For complete details on the use and execution of this protocol, please refer to Attane et al. (2020).
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spelling pubmed-82466402021-07-06 A protocol for human bone marrow adipocyte isolation and purification Attané, Camille Estève, David Moutahir, Mohamed Reina, Nicolas Muller, Catherine STAR Protoc Protocol Primary human bone marrow adipocytes (BM-Ads) display a specific metabolism that is not recapitulated by in vitro differentiated bone marrow mesenchymal stromal cells. These findings highlight the need for using primary BM-Ads in studies of the metabolic impact of BM-Ads on surrounding cells. Here, we present a protocol for isolating human BM-Ads from bone marrow aspirates and verifying adipocyte suspension purity. These isolated and purified BM-Ads can be used for functional assays or frozen for molecular analyses. For complete details on the use and execution of this protocol, please refer to Attane et al. (2020). Elsevier 2021-06-24 /pmc/articles/PMC8246640/ /pubmed/34235494 http://dx.doi.org/10.1016/j.xpro.2021.100629 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Attané, Camille
Estève, David
Moutahir, Mohamed
Reina, Nicolas
Muller, Catherine
A protocol for human bone marrow adipocyte isolation and purification
title A protocol for human bone marrow adipocyte isolation and purification
title_full A protocol for human bone marrow adipocyte isolation and purification
title_fullStr A protocol for human bone marrow adipocyte isolation and purification
title_full_unstemmed A protocol for human bone marrow adipocyte isolation and purification
title_short A protocol for human bone marrow adipocyte isolation and purification
title_sort protocol for human bone marrow adipocyte isolation and purification
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246640/
https://www.ncbi.nlm.nih.gov/pubmed/34235494
http://dx.doi.org/10.1016/j.xpro.2021.100629
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