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Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats
BACKGROUND: We investigated the role of epinephrine in prolonging the localization of lidocaine on the oral mucosa and inhibiting its absorption in the blood of rats. METHODS: We used 7–8-week-old pathogen-free Wistar male rats (n = 128) for our study. We divided them into the control group administ...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246680/ https://www.ncbi.nlm.nih.gov/pubmed/34210305 http://dx.doi.org/10.1186/s12903-021-01691-0 |
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author | Sasaki, Rui Sunada, Katsuhisa |
author_facet | Sasaki, Rui Sunada, Katsuhisa |
author_sort | Sasaki, Rui |
collection | PubMed |
description | BACKGROUND: We investigated the role of epinephrine in prolonging the localization of lidocaine on the oral mucosa and inhibiting its absorption in the blood of rats. METHODS: We used 7–8-week-old pathogen-free Wistar male rats (n = 128) for our study. We divided them into the control group administered with (14)C-labeled lidocaine hydrochloride gel only and the study group administered with (14)C-labeled lidocaine hydrochloride gel with epinephrine. The medications were administered in the palatal mucosa of the rats. The amount of mucosa, palatine bone, and serum lidocaine was measured by radioactivity using a liquid scintillation counter and was observed using autoradiograms. RESULTS: Initially, there was no significant difference in the lidocaine levels between the lidocaine and lidocaine with epinephrine groups in the palatal mucosa (751.9 ± 133.8 vs. 669.8 ± 101.6 ng/mg [2 min]). After 4 min, the values were significantly lower in the lidocaine with epinephrine group (1040.0 ± 142.8 vs. 701.2 ± 109.0 ng/mg [20 min]). After 40 min, the lidocaine level became significantly higher in the lidocaine with epinephrine group (586.8 ± 112.4 vs. 1131.3 ± 155.2 ng/mg [40 min]). Similar results were observed in the palatine bone and serum. CONCLUSION: Epinephrine prolonged the localization of lidocaine applied to the mucosa and inhibited its absorption into the bloodstream of rats. Clinical studies are required to evaluate the use of epinephrine-containing topical anesthetics on the oral mucosa. |
format | Online Article Text |
id | pubmed-8246680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82466802021-07-06 Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats Sasaki, Rui Sunada, Katsuhisa BMC Oral Health Research BACKGROUND: We investigated the role of epinephrine in prolonging the localization of lidocaine on the oral mucosa and inhibiting its absorption in the blood of rats. METHODS: We used 7–8-week-old pathogen-free Wistar male rats (n = 128) for our study. We divided them into the control group administered with (14)C-labeled lidocaine hydrochloride gel only and the study group administered with (14)C-labeled lidocaine hydrochloride gel with epinephrine. The medications were administered in the palatal mucosa of the rats. The amount of mucosa, palatine bone, and serum lidocaine was measured by radioactivity using a liquid scintillation counter and was observed using autoradiograms. RESULTS: Initially, there was no significant difference in the lidocaine levels between the lidocaine and lidocaine with epinephrine groups in the palatal mucosa (751.9 ± 133.8 vs. 669.8 ± 101.6 ng/mg [2 min]). After 4 min, the values were significantly lower in the lidocaine with epinephrine group (1040.0 ± 142.8 vs. 701.2 ± 109.0 ng/mg [20 min]). After 40 min, the lidocaine level became significantly higher in the lidocaine with epinephrine group (586.8 ± 112.4 vs. 1131.3 ± 155.2 ng/mg [40 min]). Similar results were observed in the palatine bone and serum. CONCLUSION: Epinephrine prolonged the localization of lidocaine applied to the mucosa and inhibited its absorption into the bloodstream of rats. Clinical studies are required to evaluate the use of epinephrine-containing topical anesthetics on the oral mucosa. BioMed Central 2021-07-01 /pmc/articles/PMC8246680/ /pubmed/34210305 http://dx.doi.org/10.1186/s12903-021-01691-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Sasaki, Rui Sunada, Katsuhisa Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats |
title | Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats |
title_full | Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats |
title_fullStr | Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats |
title_full_unstemmed | Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats |
title_short | Effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats |
title_sort | effect of epinephrine on the absorption of lidocaine following application to the oral mucosa in rats |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246680/ https://www.ncbi.nlm.nih.gov/pubmed/34210305 http://dx.doi.org/10.1186/s12903-021-01691-0 |
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