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The strength and form of natural selection on transcript abundance in the wild
Gene transcription variation is known to contribute to disease susceptibility and adaptation, but we currently know very little about how contemporary natural selection shapes transcript abundance. Here, we propose a novel analytical framework to quantify the strength and form of ongoing natural sel...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246785/ https://www.ncbi.nlm.nih.gov/pubmed/33219570 http://dx.doi.org/10.1111/mec.15743 |
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author | Ahmad, Freed Debes, Paul V. Nousiainen, Ilkka Kahar, Siim Pukk, Lilian Gross, Riho Ozerov, Mikhail Vasemägi, Anti |
author_facet | Ahmad, Freed Debes, Paul V. Nousiainen, Ilkka Kahar, Siim Pukk, Lilian Gross, Riho Ozerov, Mikhail Vasemägi, Anti |
author_sort | Ahmad, Freed |
collection | PubMed |
description | Gene transcription variation is known to contribute to disease susceptibility and adaptation, but we currently know very little about how contemporary natural selection shapes transcript abundance. Here, we propose a novel analytical framework to quantify the strength and form of ongoing natural selection at the transcriptome level in a wild vertebrate. We estimated selection on transcript abundance in a cohort of a wild salmonid fish (Salmo trutta) affected by an extracellular myxozoan parasite (Tetracapsuloides bryosalmonae) through mark–recapture field sampling and the integration of RNA‐sequencing with classical regression‐based selection analysis. We show, based on fin transcriptomes of the host, that infection by the parasite and subsequent host survival is linked to upregulation of mitotic cell cycle process. We also detect a widespread signal of disruptive selection on transcripts linked to host immune defence, host–pathogen interactions, cellular repair and maintenance. Our results provide insights into how selection can be measured at the transcriptome level to dissect the molecular mechanisms of contemporary evolution driven by climate change and emerging anthropogenic threats. We anticipate that the approach described here will enable critical information on the molecular processes and targets of natural selection to be obtained in real time. |
format | Online Article Text |
id | pubmed-8246785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82467852021-07-02 The strength and form of natural selection on transcript abundance in the wild Ahmad, Freed Debes, Paul V. Nousiainen, Ilkka Kahar, Siim Pukk, Lilian Gross, Riho Ozerov, Mikhail Vasemägi, Anti Mol Ecol From the Cover Gene transcription variation is known to contribute to disease susceptibility and adaptation, but we currently know very little about how contemporary natural selection shapes transcript abundance. Here, we propose a novel analytical framework to quantify the strength and form of ongoing natural selection at the transcriptome level in a wild vertebrate. We estimated selection on transcript abundance in a cohort of a wild salmonid fish (Salmo trutta) affected by an extracellular myxozoan parasite (Tetracapsuloides bryosalmonae) through mark–recapture field sampling and the integration of RNA‐sequencing with classical regression‐based selection analysis. We show, based on fin transcriptomes of the host, that infection by the parasite and subsequent host survival is linked to upregulation of mitotic cell cycle process. We also detect a widespread signal of disruptive selection on transcripts linked to host immune defence, host–pathogen interactions, cellular repair and maintenance. Our results provide insights into how selection can be measured at the transcriptome level to dissect the molecular mechanisms of contemporary evolution driven by climate change and emerging anthropogenic threats. We anticipate that the approach described here will enable critical information on the molecular processes and targets of natural selection to be obtained in real time. John Wiley and Sons Inc. 2020-12-07 2021-06 /pmc/articles/PMC8246785/ /pubmed/33219570 http://dx.doi.org/10.1111/mec.15743 Text en © 2020 The Authors. Molecular Ecology published by John Wiley & Sons Ltd https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | From the Cover Ahmad, Freed Debes, Paul V. Nousiainen, Ilkka Kahar, Siim Pukk, Lilian Gross, Riho Ozerov, Mikhail Vasemägi, Anti The strength and form of natural selection on transcript abundance in the wild |
title | The strength and form of natural selection on transcript abundance in the wild |
title_full | The strength and form of natural selection on transcript abundance in the wild |
title_fullStr | The strength and form of natural selection on transcript abundance in the wild |
title_full_unstemmed | The strength and form of natural selection on transcript abundance in the wild |
title_short | The strength and form of natural selection on transcript abundance in the wild |
title_sort | strength and form of natural selection on transcript abundance in the wild |
topic | From the Cover |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246785/ https://www.ncbi.nlm.nih.gov/pubmed/33219570 http://dx.doi.org/10.1111/mec.15743 |
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