Method for cryopreservation of Paramoeba perurans

Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long‐term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The...

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Detalles Bibliográficos
Autores principales: Tröße, Christiane, Kindt, Mats, Blindheim, Steffen, Andersen, Linda, Nylund, Are
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246932/
https://www.ncbi.nlm.nih.gov/pubmed/33159703
http://dx.doi.org/10.1111/jfd.13295
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author Tröße, Christiane
Kindt, Mats
Blindheim, Steffen
Andersen, Linda
Nylund, Are
author_facet Tröße, Christiane
Kindt, Mats
Blindheim, Steffen
Andersen, Linda
Nylund, Are
author_sort Tröße, Christiane
collection PubMed
description Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long‐term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The method was successfully employed on four out the five clones we tested. The thawing success rate, that is the percentage of successfully thawed vials relative to the total number of vials that were thawed, differed for the clones and ranged from 25% to 100%. The age of the clones seemed to have a negative impact on the ability to survive cryopreservation.
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spelling pubmed-82469322021-07-02 Method for cryopreservation of Paramoeba perurans Tröße, Christiane Kindt, Mats Blindheim, Steffen Andersen, Linda Nylund, Are J Fish Dis Original Articles Paramoeba perurans causes amoebic gill disease (AGD), which is a major problem in aquaculture worldwide. The parasite can be cultured in vitro, but to this date, no method for long‐term storage of the clones exists. In this study, we describe a method for cryopreservation of Paramoeba perurans. The method was successfully employed on four out the five clones we tested. The thawing success rate, that is the percentage of successfully thawed vials relative to the total number of vials that were thawed, differed for the clones and ranged from 25% to 100%. The age of the clones seemed to have a negative impact on the ability to survive cryopreservation. John Wiley and Sons Inc. 2020-11-07 2021-06 /pmc/articles/PMC8246932/ /pubmed/33159703 http://dx.doi.org/10.1111/jfd.13295 Text en © 2020 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Tröße, Christiane
Kindt, Mats
Blindheim, Steffen
Andersen, Linda
Nylund, Are
Method for cryopreservation of Paramoeba perurans
title Method for cryopreservation of Paramoeba perurans
title_full Method for cryopreservation of Paramoeba perurans
title_fullStr Method for cryopreservation of Paramoeba perurans
title_full_unstemmed Method for cryopreservation of Paramoeba perurans
title_short Method for cryopreservation of Paramoeba perurans
title_sort method for cryopreservation of paramoeba perurans
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246932/
https://www.ncbi.nlm.nih.gov/pubmed/33159703
http://dx.doi.org/10.1111/jfd.13295
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