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Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories
BACKGROUND: The ability of chickpea to obtain sufficient nitrogen via its symbiotic relationship with Mesorhizobium ciceri is of critical importance in supporting growth and grain production. A number of factors can affect this symbiotic relationship including abiotic conditions, plant genotype, and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247157/ https://www.ncbi.nlm.nih.gov/pubmed/34210277 http://dx.doi.org/10.1186/s12870-021-03102-6 |
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author | Plett, Krista L. Bithell, Sean L. Dando, Adrian Plett, Jonathan M. |
author_facet | Plett, Krista L. Bithell, Sean L. Dando, Adrian Plett, Jonathan M. |
author_sort | Plett, Krista L. |
collection | PubMed |
description | BACKGROUND: The ability of chickpea to obtain sufficient nitrogen via its symbiotic relationship with Mesorhizobium ciceri is of critical importance in supporting growth and grain production. A number of factors can affect this symbiotic relationship including abiotic conditions, plant genotype, and disruptions to host signalling/perception networks. In order to support improved nodule formation in chickpea, we investigated how plant genotype and soil nutrient availability affect chickpea nodule formation and nitrogen fixation. Further, using transcriptomic profiling, we sought to identify gene expression patterns that characterize highly nodulated genotypes. RESULTS: A study involving six chickpea varieties demonstrated large genotype by soil nitrogen interaction effects on nodulation and further identified agronomic traits of genotypes (such as shoot weight) associated with high nodulation. We broadened our scope to consider 29 varieties and breeding lines to examine the relationship between soilborne disease resistance and the number of nodules developed and real-time nitrogen fixation. Results of this larger study supported the earlier genotype specific findings, however, disease resistance did not explain differences in nodulation across genotypes. Transcriptional profiling of six chickpea genotypes indicates that genes associated with signalling, N transport and cellular localization, as opposed to genes associated with the classical nodulation pathway, are more likely to predict whether a given genotype will exhibit high levels of nodule formation. CONCLUSIONS: This research identified a number of key abiotic and genetic factors affecting chickpea nodule development and nitrogen fixation. These findings indicate that an improved understanding of genotype-specific factors affecting chickpea nodule induction and function are key research areas necessary to improving the benefits of rhizobial symbiosis in chickpea. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03102-6. |
format | Online Article Text |
id | pubmed-8247157 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82471572021-07-06 Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories Plett, Krista L. Bithell, Sean L. Dando, Adrian Plett, Jonathan M. BMC Plant Biol Research BACKGROUND: The ability of chickpea to obtain sufficient nitrogen via its symbiotic relationship with Mesorhizobium ciceri is of critical importance in supporting growth and grain production. A number of factors can affect this symbiotic relationship including abiotic conditions, plant genotype, and disruptions to host signalling/perception networks. In order to support improved nodule formation in chickpea, we investigated how plant genotype and soil nutrient availability affect chickpea nodule formation and nitrogen fixation. Further, using transcriptomic profiling, we sought to identify gene expression patterns that characterize highly nodulated genotypes. RESULTS: A study involving six chickpea varieties demonstrated large genotype by soil nitrogen interaction effects on nodulation and further identified agronomic traits of genotypes (such as shoot weight) associated with high nodulation. We broadened our scope to consider 29 varieties and breeding lines to examine the relationship between soilborne disease resistance and the number of nodules developed and real-time nitrogen fixation. Results of this larger study supported the earlier genotype specific findings, however, disease resistance did not explain differences in nodulation across genotypes. Transcriptional profiling of six chickpea genotypes indicates that genes associated with signalling, N transport and cellular localization, as opposed to genes associated with the classical nodulation pathway, are more likely to predict whether a given genotype will exhibit high levels of nodule formation. CONCLUSIONS: This research identified a number of key abiotic and genetic factors affecting chickpea nodule development and nitrogen fixation. These findings indicate that an improved understanding of genotype-specific factors affecting chickpea nodule induction and function are key research areas necessary to improving the benefits of rhizobial symbiosis in chickpea. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03102-6. BioMed Central 2021-07-01 /pmc/articles/PMC8247157/ /pubmed/34210277 http://dx.doi.org/10.1186/s12870-021-03102-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Plett, Krista L. Bithell, Sean L. Dando, Adrian Plett, Jonathan M. Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories |
title | Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories |
title_full | Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories |
title_fullStr | Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories |
title_full_unstemmed | Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories |
title_short | Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories |
title_sort | chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247157/ https://www.ncbi.nlm.nih.gov/pubmed/34210277 http://dx.doi.org/10.1186/s12870-021-03102-6 |
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