Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia

Given its myeloid‐restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow prot...

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Autores principales: Bras, Anne E., Osmani, Zgjim, de Haas, Valerie, Jongen‐Lavrencic, Mojca, te Marvelde, Jeroen G., Zwaan, C. Michel, Mejstrikova, Ester, Fernandez, Paula, Szczepanski, Tomasz, Orfao, Alberto, van Dongen, Jacques J. M., van der Velden, Vincent H. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Haematological malignancy – Clinical
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247412/
https://www.ncbi.nlm.nih.gov/pubmed/33161592
http://dx.doi.org/10.1111/bjh.17210
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author Bras, Anne E.
Osmani, Zgjim
de Haas, Valerie
Jongen‐Lavrencic, Mojca
te Marvelde, Jeroen G.
Zwaan, C. Michel
Mejstrikova, Ester
Fernandez, Paula
Szczepanski, Tomasz
Orfao, Alberto
van Dongen, Jacques J. M.
van der Velden, Vincent H. J.
author_facet Bras, Anne E.
Osmani, Zgjim
de Haas, Valerie
Jongen‐Lavrencic, Mojca
te Marvelde, Jeroen G.
Zwaan, C. Michel
Mejstrikova, Ester
Fernandez, Paula
Szczepanski, Tomasz
Orfao, Alberto
van Dongen, Jacques J. M.
van der Velden, Vincent H. J.
author_sort Bras, Anne E.
collection PubMed
description Given its myeloid‐restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte‐based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.
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spelling pubmed-82474122021-07-02 Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia Bras, Anne E. Osmani, Zgjim de Haas, Valerie Jongen‐Lavrencic, Mojca te Marvelde, Jeroen G. Zwaan, C. Michel Mejstrikova, Ester Fernandez, Paula Szczepanski, Tomasz Orfao, Alberto van Dongen, Jacques J. M. van der Velden, Vincent H. J. Br J Haematol Haematological malignancy – Clinical Given its myeloid‐restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte‐based threshold. The methodology employed should be applicable to any form of standardised flow cytometry. John Wiley and Sons Inc. 2020-11-07 2021-06 /pmc/articles/PMC8247412/ /pubmed/33161592 http://dx.doi.org/10.1111/bjh.17210 Text en © 2020 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Haematological malignancy – Clinical
Bras, Anne E.
Osmani, Zgjim
de Haas, Valerie
Jongen‐Lavrencic, Mojca
te Marvelde, Jeroen G.
Zwaan, C. Michel
Mejstrikova, Ester
Fernandez, Paula
Szczepanski, Tomasz
Orfao, Alberto
van Dongen, Jacques J. M.
van der Velden, Vincent H. J.
Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
title Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
title_full Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
title_fullStr Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
title_full_unstemmed Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
title_short Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
title_sort standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia
topic Haematological malignancy – Clinical
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247412/
https://www.ncbi.nlm.nih.gov/pubmed/33161592
http://dx.doi.org/10.1111/bjh.17210
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