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Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum

BACKGROUND: Sugarcane is a crop of global importance and has been expanding to areas with soils containing high levels of exchangeable aluminum (Al), which is a limiting factor for crop development in acidic soils. The study of the sugarcane physiological and nutritional behavior together with patte...

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Autores principales: Oliveira, Mariane de Souza, Rocha, Sâmara Vieira, Schneider, Vanessa Karine, Henrique-Silva, Flavio, Soares, Marcio Roberto, Soares-Costa, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247702/
https://www.ncbi.nlm.nih.gov/pubmed/34249482
http://dx.doi.org/10.7717/peerj.11461
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author Oliveira, Mariane de Souza
Rocha, Sâmara Vieira
Schneider, Vanessa Karine
Henrique-Silva, Flavio
Soares, Marcio Roberto
Soares-Costa, Andrea
author_facet Oliveira, Mariane de Souza
Rocha, Sâmara Vieira
Schneider, Vanessa Karine
Henrique-Silva, Flavio
Soares, Marcio Roberto
Soares-Costa, Andrea
author_sort Oliveira, Mariane de Souza
collection PubMed
description BACKGROUND: Sugarcane is a crop of global importance and has been expanding to areas with soils containing high levels of exchangeable aluminum (Al), which is a limiting factor for crop development in acidic soils. The study of the sugarcane physiological and nutritional behavior together with patterns of gene expression in response to Al stress may provide a basis for effective strategies to increase crop productivity in acidic soils. METHODS: Sugarcane cultivars were evaluated for physiological parameters (photosynthesis, stomatal conductance, and transpiration), nutrient (N, P, K, Ca, Mg, and S) and Al contents in leaves and roots and gene expression, of the genes MDH, SDH by qPCR, both related to the production of organic acids, and SOD, related to oxidative stress. RESULTS: Brazilian sugarcane RB867515, RB928064, and RB935744 cultivars exhibited very different responses to induced stress by Al. Exposure to Al caused up-regulation (SOD and MDH) or down-regulation (SDH, MDH, and SOD), depending on the cultivar, Al level, and plant tissue. The RB867515 cultivar was the most Al-tolerant, showing no decline of nutrient content in plant tissue, photosynthesis, transpiration, and stomatal conductance after exposure to Al; it exhibited the highest Al content in the roots, and showed important MDH and SOD gene expression in the roots. RB928064 only showed low expression of SOD in roots and leaves, while RB935744 showed important expression of the SOD gene only in the leaves. Sugarcane cultivars were classified in the following descending Al-tolerance order: RB867515 > RB928064 = RB935744. These results may contribute to the obtention of Al-tolerant cultivars that can play their genetic potential in soils of low fertility and with low demand for agricultural inputs; the selection of potential plants for breeding programs; the elucidation of Al detoxification mechanisms employed by sugarcane cultivars.
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spelling pubmed-82477022021-07-08 Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum Oliveira, Mariane de Souza Rocha, Sâmara Vieira Schneider, Vanessa Karine Henrique-Silva, Flavio Soares, Marcio Roberto Soares-Costa, Andrea PeerJ Agricultural Science BACKGROUND: Sugarcane is a crop of global importance and has been expanding to areas with soils containing high levels of exchangeable aluminum (Al), which is a limiting factor for crop development in acidic soils. The study of the sugarcane physiological and nutritional behavior together with patterns of gene expression in response to Al stress may provide a basis for effective strategies to increase crop productivity in acidic soils. METHODS: Sugarcane cultivars were evaluated for physiological parameters (photosynthesis, stomatal conductance, and transpiration), nutrient (N, P, K, Ca, Mg, and S) and Al contents in leaves and roots and gene expression, of the genes MDH, SDH by qPCR, both related to the production of organic acids, and SOD, related to oxidative stress. RESULTS: Brazilian sugarcane RB867515, RB928064, and RB935744 cultivars exhibited very different responses to induced stress by Al. Exposure to Al caused up-regulation (SOD and MDH) or down-regulation (SDH, MDH, and SOD), depending on the cultivar, Al level, and plant tissue. The RB867515 cultivar was the most Al-tolerant, showing no decline of nutrient content in plant tissue, photosynthesis, transpiration, and stomatal conductance after exposure to Al; it exhibited the highest Al content in the roots, and showed important MDH and SOD gene expression in the roots. RB928064 only showed low expression of SOD in roots and leaves, while RB935744 showed important expression of the SOD gene only in the leaves. Sugarcane cultivars were classified in the following descending Al-tolerance order: RB867515 > RB928064 = RB935744. These results may contribute to the obtention of Al-tolerant cultivars that can play their genetic potential in soils of low fertility and with low demand for agricultural inputs; the selection of potential plants for breeding programs; the elucidation of Al detoxification mechanisms employed by sugarcane cultivars. PeerJ Inc. 2021-06-28 /pmc/articles/PMC8247702/ /pubmed/34249482 http://dx.doi.org/10.7717/peerj.11461 Text en © 2021 Oliveira et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Oliveira, Mariane de Souza
Rocha, Sâmara Vieira
Schneider, Vanessa Karine
Henrique-Silva, Flavio
Soares, Marcio Roberto
Soares-Costa, Andrea
Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum
title Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum
title_full Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum
title_fullStr Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum
title_full_unstemmed Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum
title_short Physiological, nutritional, and molecular responses of Brazilian sugarcane cultivars under stress by aluminum
title_sort physiological, nutritional, and molecular responses of brazilian sugarcane cultivars under stress by aluminum
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247702/
https://www.ncbi.nlm.nih.gov/pubmed/34249482
http://dx.doi.org/10.7717/peerj.11461
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