Maize metabolome and proteome responses to controlled cold stress partly mimic early‐sowing effects in the field and differ from those of Arabidopsis

In Northern Europe, sowing maize one‐month earlier than current agricultural practices may lead to moderate chilling damage. However, studies of the metabolic responses to low, non‐freezing, temperatures remain scarce. Here, genetically‐diverse maize hybrids (Zea mays, dent inbred lines crossed with a flint inbred line) were cultivated in a growth chamber at optimal temperature and then three decreasing temperatures for 2 days each, as well as in the field. Leaf metabolomic and proteomic profiles were determined. In the growth chamber, 50% of metabolites and 18% of proteins changed between 20 and 16°C. These maize responses, partly differing from those of Arabidopsis to short‐term chilling, were mapped on genome‐wide metabolic maps. Several metabolites and proteins showed similar variation for all temperature decreases: seven MS‐based metabolite signatures and two proteins involved in photosynthesis decreased continuously. Several increasing metabolites or proteins in the growth‐chamber chilling conditions showed similar trends in the early‐sowing field experiment, including trans‐aconitate, three hydroxycinnamate derivatives, a benzoxazinoid, a sucrose synthase, lethal leaf‐spot 1 protein, an allene oxide synthase, several glutathione transferases and peroxidases. Hybrid groups based on field biomass were used to search for the metabolite or protein responses differentiating them in growth‐chamber conditions, which could be of interest for breeding.