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Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes

Laccases are multi‐copper oxidases that catalyze the oxidation of various electron‐rich substrates with concomitant reduction of molecular oxygen to water. The multi‐copper oxidase/laccase CueO of Escherichia coli is responsible for the oxidation of Cu(+) to the less harmful Cu(2+) in the periplasm....

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Autores principales: Decembrino, Davide, Girhard, Marco, Urlacher, Vlada B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248233/
https://www.ncbi.nlm.nih.gov/pubmed/33332702
http://dx.doi.org/10.1002/cbic.202000775
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author Decembrino, Davide
Girhard, Marco
Urlacher, Vlada B.
author_facet Decembrino, Davide
Girhard, Marco
Urlacher, Vlada B.
author_sort Decembrino, Davide
collection PubMed
description Laccases are multi‐copper oxidases that catalyze the oxidation of various electron‐rich substrates with concomitant reduction of molecular oxygen to water. The multi‐copper oxidase/laccase CueO of Escherichia coli is responsible for the oxidation of Cu(+) to the less harmful Cu(2+) in the periplasm. CueO has a relatively broad substrate spectrum as laccase, and its activity is enhanced by copper excess. The aim of this study was to trigger CueO activity in vivo for the use in biocatalysis. The addition of 5 mM CuSO(4) was proven effective in triggering CueO activity at need with minor toxic effects on E. coli cells. Cu‐treated E. coli cells were able to convert several phenolic compounds to the corresponding dimers. Finally, the endogenous CueO activity was applied to a four‐step cascade, in which coniferyl alcohol was converted to the valuable plant lignan (−)‐matairesinol.
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spelling pubmed-82482332021-07-06 Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes Decembrino, Davide Girhard, Marco Urlacher, Vlada B. Chembiochem Full Papers Laccases are multi‐copper oxidases that catalyze the oxidation of various electron‐rich substrates with concomitant reduction of molecular oxygen to water. The multi‐copper oxidase/laccase CueO of Escherichia coli is responsible for the oxidation of Cu(+) to the less harmful Cu(2+) in the periplasm. CueO has a relatively broad substrate spectrum as laccase, and its activity is enhanced by copper excess. The aim of this study was to trigger CueO activity in vivo for the use in biocatalysis. The addition of 5 mM CuSO(4) was proven effective in triggering CueO activity at need with minor toxic effects on E. coli cells. Cu‐treated E. coli cells were able to convert several phenolic compounds to the corresponding dimers. Finally, the endogenous CueO activity was applied to a four‐step cascade, in which coniferyl alcohol was converted to the valuable plant lignan (−)‐matairesinol. John Wiley and Sons Inc. 2021-02-04 2021-04-16 /pmc/articles/PMC8248233/ /pubmed/33332702 http://dx.doi.org/10.1002/cbic.202000775 Text en © 2020 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Full Papers
Decembrino, Davide
Girhard, Marco
Urlacher, Vlada B.
Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes
title Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes
title_full Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes
title_fullStr Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes
title_full_unstemmed Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes
title_short Use of Copper as a Trigger for the in Vivo Activity of E. coli Laccase CueO: A Simple Tool for Biosynthetic Purposes
title_sort use of copper as a trigger for the in vivo activity of e. coli laccase cueo: a simple tool for biosynthetic purposes
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248233/
https://www.ncbi.nlm.nih.gov/pubmed/33332702
http://dx.doi.org/10.1002/cbic.202000775
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