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Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy

Point‐of‐care (POC) diagnostic tests for the rapid detection of individuals infected with Mycobacterium leprae, the causative pathogen of leprosy, represent efficient tools to guide therapeutic and prophylactic treatment strategies in leprosy control programs, thus positively contributing to clinica...

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Autores principales: van Dijk, J. Hessel M., van Hooij, Anouk, Groot, L. Melanie, Geboers, Jolijn, Moretti, Rosita, Verhard‐Seymonsbergen, Els, de Jong, Danielle, van der Marel, Gijs A., Corstjens, Paul L. A. M., Codée, Jeroen D. C., Geluk, Annemieke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248333/
https://www.ncbi.nlm.nih.gov/pubmed/33332701
http://dx.doi.org/10.1002/cbic.202000810
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author van Dijk, J. Hessel M.
van Hooij, Anouk
Groot, L. Melanie
Geboers, Jolijn
Moretti, Rosita
Verhard‐Seymonsbergen, Els
de Jong, Danielle
van der Marel, Gijs A.
Corstjens, Paul L. A. M.
Codée, Jeroen D. C.
Geluk, Annemieke
author_facet van Dijk, J. Hessel M.
van Hooij, Anouk
Groot, L. Melanie
Geboers, Jolijn
Moretti, Rosita
Verhard‐Seymonsbergen, Els
de Jong, Danielle
van der Marel, Gijs A.
Corstjens, Paul L. A. M.
Codée, Jeroen D. C.
Geluk, Annemieke
author_sort van Dijk, J. Hessel M.
collection PubMed
description Point‐of‐care (POC) diagnostic tests for the rapid detection of individuals infected with Mycobacterium leprae, the causative pathogen of leprosy, represent efficient tools to guide therapeutic and prophylactic treatment strategies in leprosy control programs, thus positively contributing to clinical outcome and reducing transmission of this infectious disease. Levels of antibodies directed against the M. leprae‐specific phenolic glycolipid I (PGL−I) closely correlate with an individual's bacterial load and a higher risk of developing leprosy. We describe herein the assembly of a set of PGL glycans carrying the characteristic phenol aglycon and featuring different methylation patterns. The PGL trisaccharides were applied to construct neoglycoproteins that were used to detect anti‐PGL IgM antibodies in leprosy patients. ELISAs and quantitative lateral‐flow assays based on up‐converting nanoparticles (UCP‐LFAs) showed that the generated PGL−I and PGL‐II trisaccharide neoglycoconjugates can be applied for the detection of anti M. leprae IgM antibodies in POC tests.
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spelling pubmed-82483332021-07-06 Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy van Dijk, J. Hessel M. van Hooij, Anouk Groot, L. Melanie Geboers, Jolijn Moretti, Rosita Verhard‐Seymonsbergen, Els de Jong, Danielle van der Marel, Gijs A. Corstjens, Paul L. A. M. Codée, Jeroen D. C. Geluk, Annemieke Chembiochem Full Papers Point‐of‐care (POC) diagnostic tests for the rapid detection of individuals infected with Mycobacterium leprae, the causative pathogen of leprosy, represent efficient tools to guide therapeutic and prophylactic treatment strategies in leprosy control programs, thus positively contributing to clinical outcome and reducing transmission of this infectious disease. Levels of antibodies directed against the M. leprae‐specific phenolic glycolipid I (PGL−I) closely correlate with an individual's bacterial load and a higher risk of developing leprosy. We describe herein the assembly of a set of PGL glycans carrying the characteristic phenol aglycon and featuring different methylation patterns. The PGL trisaccharides were applied to construct neoglycoproteins that were used to detect anti‐PGL IgM antibodies in leprosy patients. ELISAs and quantitative lateral‐flow assays based on up‐converting nanoparticles (UCP‐LFAs) showed that the generated PGL−I and PGL‐II trisaccharide neoglycoconjugates can be applied for the detection of anti M. leprae IgM antibodies in POC tests. John Wiley and Sons Inc. 2021-02-10 2021-04-16 /pmc/articles/PMC8248333/ /pubmed/33332701 http://dx.doi.org/10.1002/cbic.202000810 Text en © 2020 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Full Papers
van Dijk, J. Hessel M.
van Hooij, Anouk
Groot, L. Melanie
Geboers, Jolijn
Moretti, Rosita
Verhard‐Seymonsbergen, Els
de Jong, Danielle
van der Marel, Gijs A.
Corstjens, Paul L. A. M.
Codée, Jeroen D. C.
Geluk, Annemieke
Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy
title Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy
title_full Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy
title_fullStr Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy
title_full_unstemmed Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy
title_short Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy
title_sort synthetic phenolic glycolipids for application in diagnostic tests for leprosy
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248333/
https://www.ncbi.nlm.nih.gov/pubmed/33332701
http://dx.doi.org/10.1002/cbic.202000810
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