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Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats

Following skeletal muscle injury (SMI), from post-injury reaction to repair consists of a complex series of dynamic changes. However, there is a paucity of research on detailed transcriptional dynamics and time-dependent marker gene expression in the early stages after SMI. In this study, skeletal m...

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Autores principales: Ren, Kang, Wang, Liangliang, Wang, Liang, Du, Qiuxiang, Cao, Jie, Jin, Qianqian, An, Guoshuai, Li, Na, Dang, Lihong, Tian, Yingjie, Wang, Yingyuan, Sun, Junhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248501/
https://www.ncbi.nlm.nih.gov/pubmed/34220936
http://dx.doi.org/10.3389/fgene.2021.650874
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author Ren, Kang
Wang, Liangliang
Wang, Liang
Du, Qiuxiang
Cao, Jie
Jin, Qianqian
An, Guoshuai
Li, Na
Dang, Lihong
Tian, Yingjie
Wang, Yingyuan
Sun, Junhong
author_facet Ren, Kang
Wang, Liangliang
Wang, Liang
Du, Qiuxiang
Cao, Jie
Jin, Qianqian
An, Guoshuai
Li, Na
Dang, Lihong
Tian, Yingjie
Wang, Yingyuan
Sun, Junhong
author_sort Ren, Kang
collection PubMed
description Following skeletal muscle injury (SMI), from post-injury reaction to repair consists of a complex series of dynamic changes. However, there is a paucity of research on detailed transcriptional dynamics and time-dependent marker gene expression in the early stages after SMI. In this study, skeletal muscle tissue in rats was taken at 4 to 48 h after injury for next-generation sequencing. We examined the transcriptional kinetics characteristics during above time periods after injury. STEM and maSigPro were used to screen time-correlated genes. Integrating 188 time-correlated genes with 161 genes in each time-related gene module by WGCNA, we finally identified 18 network-node regulatory genes after SMI. Histological staining analyses confirmed the mechanisms underlying changes in the tissue damage to repair process. Our research linked a variety of dynamic biological processes with specific time periods and provided insight into the characteristics of transcriptional dynamics, as well as screened time-related biological indicators with biological significance in the early stages after SMI.
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spelling pubmed-82485012021-07-02 Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats Ren, Kang Wang, Liangliang Wang, Liang Du, Qiuxiang Cao, Jie Jin, Qianqian An, Guoshuai Li, Na Dang, Lihong Tian, Yingjie Wang, Yingyuan Sun, Junhong Front Genet Genetics Following skeletal muscle injury (SMI), from post-injury reaction to repair consists of a complex series of dynamic changes. However, there is a paucity of research on detailed transcriptional dynamics and time-dependent marker gene expression in the early stages after SMI. In this study, skeletal muscle tissue in rats was taken at 4 to 48 h after injury for next-generation sequencing. We examined the transcriptional kinetics characteristics during above time periods after injury. STEM and maSigPro were used to screen time-correlated genes. Integrating 188 time-correlated genes with 161 genes in each time-related gene module by WGCNA, we finally identified 18 network-node regulatory genes after SMI. Histological staining analyses confirmed the mechanisms underlying changes in the tissue damage to repair process. Our research linked a variety of dynamic biological processes with specific time periods and provided insight into the characteristics of transcriptional dynamics, as well as screened time-related biological indicators with biological significance in the early stages after SMI. Frontiers Media S.A. 2021-06-17 /pmc/articles/PMC8248501/ /pubmed/34220936 http://dx.doi.org/10.3389/fgene.2021.650874 Text en Copyright © 2021 Ren, Wang, Wang, Du, Cao, Jin, An, Li, Dang, Tian, Wang and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Ren, Kang
Wang, Liangliang
Wang, Liang
Du, Qiuxiang
Cao, Jie
Jin, Qianqian
An, Guoshuai
Li, Na
Dang, Lihong
Tian, Yingjie
Wang, Yingyuan
Sun, Junhong
Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats
title Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats
title_full Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats
title_fullStr Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats
title_full_unstemmed Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats
title_short Investigating Transcriptional Dynamics Changes and Time-Dependent Marker Gene Expression in the Early Period After Skeletal Muscle Injury in Rats
title_sort investigating transcriptional dynamics changes and time-dependent marker gene expression in the early period after skeletal muscle injury in rats
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248501/
https://www.ncbi.nlm.nih.gov/pubmed/34220936
http://dx.doi.org/10.3389/fgene.2021.650874
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