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Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties

The contamination of dental unit waterlines (DUWLs) is a serious problem and directly affects the dental care. This study aims to explore the microbial community of biofilm in DUWL from different specialties and investigate the associated factors. A total of 36 biofilm samples from 18 DUWL of six sp...

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Autores principales: Fan, Cancan, Gu, Haijing, Liu, Limin, Zhu, Haiwei, Yan, Juan, Huo, Yongbiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248794/
https://www.ncbi.nlm.nih.gov/pubmed/34222041
http://dx.doi.org/10.3389/fcimb.2021.670211
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author Fan, Cancan
Gu, Haijing
Liu, Limin
Zhu, Haiwei
Yan, Juan
Huo, Yongbiao
author_facet Fan, Cancan
Gu, Haijing
Liu, Limin
Zhu, Haiwei
Yan, Juan
Huo, Yongbiao
author_sort Fan, Cancan
collection PubMed
description The contamination of dental unit waterlines (DUWLs) is a serious problem and directly affects the dental care. This study aims to explore the microbial community of biofilm in DUWL from different specialties and investigate the associated factors. A total of 36 biofilm samples from 18 DUWL of six specialties (i.e., prosthodontics, orthodontics, pediatrics, endodontics, oral surgery, and periodontics) at two time points (i.e., before and after daily dental practice) were collected with a novel method. Genomic DNA of samples was extracted, and then 16S ribosomal DNA (rDNA) (V3–V4 regions) and ITS2 gene were amplified and sequenced. Kruskal–Wallis and Wilcoxon rank test were adopted for statistical analysis. Microbial community with high diversity of bacteria (631 genera), fungi (193 genera), and viridiplantae was detected in the biofilm samples. Proteobacteria was the dominant bacteria (representing over 65.74–95.98% of the total sequences), and the dominant fungi was Ascomycota (93.9–99.3%). Microorganisms belonging to multiple genera involved in human diseases were detected including 25 genera of bacteria and eight genera of fungi, with relative abundance of six genera over 1% (i.e., Acinetobacter, Pseudomonas, Enterobacter, Aspergillus, Candida, and Penicillium). The biofilm microbiome may be influenced by the characteristics of dental specialty and routine work to some extent. The age of dental chair unit and overall number of patients had the strongest impact on the overall bacteria composition, and the effect of daily dental practices (associated with number of patients and dental specialty) on the fungi composition was the greatest. For the first time, biofilm in DUWL related to dental specialty was comprehensively evaluated, with more abundance of bacterial and fungal communities than in water samples. Biofilm accumulation with daily work and multiple kinds of opportunistic pathogen emphasized the infectious risk with dental care and the importance of biofilm control.
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spelling pubmed-82487942021-07-02 Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties Fan, Cancan Gu, Haijing Liu, Limin Zhu, Haiwei Yan, Juan Huo, Yongbiao Front Cell Infect Microbiol Cellular and Infection Microbiology The contamination of dental unit waterlines (DUWLs) is a serious problem and directly affects the dental care. This study aims to explore the microbial community of biofilm in DUWL from different specialties and investigate the associated factors. A total of 36 biofilm samples from 18 DUWL of six specialties (i.e., prosthodontics, orthodontics, pediatrics, endodontics, oral surgery, and periodontics) at two time points (i.e., before and after daily dental practice) were collected with a novel method. Genomic DNA of samples was extracted, and then 16S ribosomal DNA (rDNA) (V3–V4 regions) and ITS2 gene were amplified and sequenced. Kruskal–Wallis and Wilcoxon rank test were adopted for statistical analysis. Microbial community with high diversity of bacteria (631 genera), fungi (193 genera), and viridiplantae was detected in the biofilm samples. Proteobacteria was the dominant bacteria (representing over 65.74–95.98% of the total sequences), and the dominant fungi was Ascomycota (93.9–99.3%). Microorganisms belonging to multiple genera involved in human diseases were detected including 25 genera of bacteria and eight genera of fungi, with relative abundance of six genera over 1% (i.e., Acinetobacter, Pseudomonas, Enterobacter, Aspergillus, Candida, and Penicillium). The biofilm microbiome may be influenced by the characteristics of dental specialty and routine work to some extent. The age of dental chair unit and overall number of patients had the strongest impact on the overall bacteria composition, and the effect of daily dental practices (associated with number of patients and dental specialty) on the fungi composition was the greatest. For the first time, biofilm in DUWL related to dental specialty was comprehensively evaluated, with more abundance of bacterial and fungal communities than in water samples. Biofilm accumulation with daily work and multiple kinds of opportunistic pathogen emphasized the infectious risk with dental care and the importance of biofilm control. Frontiers Media S.A. 2021-06-17 /pmc/articles/PMC8248794/ /pubmed/34222041 http://dx.doi.org/10.3389/fcimb.2021.670211 Text en Copyright © 2021 Fan, Gu, Liu, Zhu, Yan and Huo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Fan, Cancan
Gu, Haijing
Liu, Limin
Zhu, Haiwei
Yan, Juan
Huo, Yongbiao
Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties
title Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties
title_full Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties
title_fullStr Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties
title_full_unstemmed Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties
title_short Distinct Microbial Community of Accumulated Biofilm in Dental Unit Waterlines of Different Specialties
title_sort distinct microbial community of accumulated biofilm in dental unit waterlines of different specialties
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248794/
https://www.ncbi.nlm.nih.gov/pubmed/34222041
http://dx.doi.org/10.3389/fcimb.2021.670211
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