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Structure of an H3N2 influenza virus nucleoprotein
Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structur...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248822/ https://www.ncbi.nlm.nih.gov/pubmed/34196611 http://dx.doi.org/10.1107/S2053230X2100635X |
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author | Knight, Michael L. Fan, Haitian Bauer, David L. V. Grimes, Jonathan M. Fodor, Ervin Keown, Jeremy R. |
author_facet | Knight, Michael L. Fan, Haitian Bauer, David L. V. Grimes, Jonathan M. Fodor, Ervin Keown, Jeremy R. |
author_sort | Knight, Michael L. |
collection | PubMed |
description | Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73–90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza. |
format | Online Article Text |
id | pubmed-8248822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-82488222021-07-12 Structure of an H3N2 influenza virus nucleoprotein Knight, Michael L. Fan, Haitian Bauer, David L. V. Grimes, Jonathan M. Fodor, Ervin Keown, Jeremy R. Acta Crystallogr F Struct Biol Commun Research Communications Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73–90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza. International Union of Crystallography 2021-06-29 /pmc/articles/PMC8248822/ /pubmed/34196611 http://dx.doi.org/10.1107/S2053230X2100635X Text en © Michael L. Knight et al. 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Communications Knight, Michael L. Fan, Haitian Bauer, David L. V. Grimes, Jonathan M. Fodor, Ervin Keown, Jeremy R. Structure of an H3N2 influenza virus nucleoprotein |
title | Structure of an H3N2 influenza virus nucleoprotein |
title_full | Structure of an H3N2 influenza virus nucleoprotein |
title_fullStr | Structure of an H3N2 influenza virus nucleoprotein |
title_full_unstemmed | Structure of an H3N2 influenza virus nucleoprotein |
title_short | Structure of an H3N2 influenza virus nucleoprotein |
title_sort | structure of an h3n2 influenza virus nucleoprotein |
topic | Research Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248822/ https://www.ncbi.nlm.nih.gov/pubmed/34196611 http://dx.doi.org/10.1107/S2053230X2100635X |
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