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Structure of an H3N2 influenza virus nucleoprotein

Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structur...

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Autores principales: Knight, Michael L., Fan, Haitian, Bauer, David L. V., Grimes, Jonathan M., Fodor, Ervin, Keown, Jeremy R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248822/
https://www.ncbi.nlm.nih.gov/pubmed/34196611
http://dx.doi.org/10.1107/S2053230X2100635X
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author Knight, Michael L.
Fan, Haitian
Bauer, David L. V.
Grimes, Jonathan M.
Fodor, Ervin
Keown, Jeremy R.
author_facet Knight, Michael L.
Fan, Haitian
Bauer, David L. V.
Grimes, Jonathan M.
Fodor, Ervin
Keown, Jeremy R.
author_sort Knight, Michael L.
collection PubMed
description Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73–90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza.
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spelling pubmed-82488222021-07-12 Structure of an H3N2 influenza virus nucleoprotein Knight, Michael L. Fan, Haitian Bauer, David L. V. Grimes, Jonathan M. Fodor, Ervin Keown, Jeremy R. Acta Crystallogr F Struct Biol Commun Research Communications Influenza A viruses of the H1N1 and H3N2 subtypes are responsible for seasonal epidemic events. The influenza nucleoprotein (NP) binds to the viral genomic RNA and is essential for its replication. Efforts are under way to produce therapeutics and vaccines targeting the NP. Despite this, no structure of an NP from an H3N2 virus has previously been determined. Here, the structure of the A/Northern Territory/60/1968 (H3N2) influenza virus NP is presented at 2.2 Å resolution. The structure is highly similar to those of the A/WSN/1933 (H1N1) and A/Hong Kong/483/97 (H5N1) NPs. Nonconserved amino acids are widely dispersed both at the sequence and structural levels. A movement of the 73–90 RNA-binding loop is observed to be the key difference between the structure determined here and previous structures. The data presented here increase the understanding of structural conservation amongst influenza NPs and may aid in the design of universal interventions against influenza. International Union of Crystallography 2021-06-29 /pmc/articles/PMC8248822/ /pubmed/34196611 http://dx.doi.org/10.1107/S2053230X2100635X Text en © Michael L. Knight et al. 2021 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Communications
Knight, Michael L.
Fan, Haitian
Bauer, David L. V.
Grimes, Jonathan M.
Fodor, Ervin
Keown, Jeremy R.
Structure of an H3N2 influenza virus nucleoprotein
title Structure of an H3N2 influenza virus nucleoprotein
title_full Structure of an H3N2 influenza virus nucleoprotein
title_fullStr Structure of an H3N2 influenza virus nucleoprotein
title_full_unstemmed Structure of an H3N2 influenza virus nucleoprotein
title_short Structure of an H3N2 influenza virus nucleoprotein
title_sort structure of an h3n2 influenza virus nucleoprotein
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248822/
https://www.ncbi.nlm.nih.gov/pubmed/34196611
http://dx.doi.org/10.1107/S2053230X2100635X
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