A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform

Abstract: During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functio...

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Autores principales: Liu, Ya, Zhao, Changhong, Sabirsh, Alan, Ye, Lilei, Wu, Xiaoqiu, Lu, Hongbin, Liu, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248920/
https://www.ncbi.nlm.nih.gov/pubmed/33829677
http://dx.doi.org/10.1002/open.202000200
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author Liu, Ya
Zhao, Changhong
Sabirsh, Alan
Ye, Lilei
Wu, Xiaoqiu
Lu, Hongbin
Liu, Johan
author_facet Liu, Ya
Zhao, Changhong
Sabirsh, Alan
Ye, Lilei
Wu, Xiaoqiu
Lu, Hongbin
Liu, Johan
author_sort Liu, Ya
collection PubMed
description Abstract: During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh‐7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first‐generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.
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spelling pubmed-82489202021-07-09 A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform Liu, Ya Zhao, Changhong Sabirsh, Alan Ye, Lilei Wu, Xiaoqiu Lu, Hongbin Liu, Johan ChemistryOpen Full Papers Abstract: During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh‐7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first‐generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective. John Wiley and Sons Inc. 2021-04-07 /pmc/articles/PMC8248920/ /pubmed/33829677 http://dx.doi.org/10.1002/open.202000200 Text en © 2021 The Authors. Published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Full Papers
Liu, Ya
Zhao, Changhong
Sabirsh, Alan
Ye, Lilei
Wu, Xiaoqiu
Lu, Hongbin
Liu, Johan
A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform
title A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform
title_full A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform
title_fullStr A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform
title_full_unstemmed A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform
title_short A Novel Graphene Quantum Dot‐Based mRNA Delivery Platform
title_sort novel graphene quantum dot‐based mrna delivery platform
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8248920/
https://www.ncbi.nlm.nih.gov/pubmed/33829677
http://dx.doi.org/10.1002/open.202000200
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