Cargando…
Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments
Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed using five...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249597/ https://www.ncbi.nlm.nih.gov/pubmed/34210979 http://dx.doi.org/10.1038/s41538-021-00097-0 |
_version_ | 1783716930244313088 |
---|---|
author | Zhang, Peipei Essendoubi, Saida Keenliside, Julia Reuter, Tim Stanford, Kim King, Robin Lu, Patricia Yang, Xianqin |
author_facet | Zhang, Peipei Essendoubi, Saida Keenliside, Julia Reuter, Tim Stanford, Kim King, Robin Lu, Patricia Yang, Xianqin |
author_sort | Zhang, Peipei |
collection | PubMed |
description | Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed using five conventional subtyping methods, with most or all strains being in one subgroup. Whole-genome single nucleotide polymorphism analysis confirmed the recent ancestry of the isolates from all three sources. Most environmental isolates clustered closer with pig isolates than cattle isolates. Also, a direct link was observed between 2018-outbreak environmental isolates and isolates collected from a pig farm in 2018. The majority of pig isolates harbor only one Shiga toxin gene, stx(2a), while 70% (35/50) of the cattle isolates have both stx(1a) and stx(2a). The results show some E. coli O157:H7 strains could establish persistence on pig farms and as such, pigs can be a significant source of the organism. |
format | Online Article Text |
id | pubmed-8249597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82495972021-07-19 Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments Zhang, Peipei Essendoubi, Saida Keenliside, Julia Reuter, Tim Stanford, Kim King, Robin Lu, Patricia Yang, Xianqin NPJ Sci Food Article Three E. coli O157:H7 outbreaks have been attributed to contaminated pork in Alberta, Canada, recently. This study investigates the phylogenetic relatedness of E. coli O157:H7 from pigs, cattle, and pork-production environments for source attribution. Limited strain diversity was observed using five conventional subtyping methods, with most or all strains being in one subgroup. Whole-genome single nucleotide polymorphism analysis confirmed the recent ancestry of the isolates from all three sources. Most environmental isolates clustered closer with pig isolates than cattle isolates. Also, a direct link was observed between 2018-outbreak environmental isolates and isolates collected from a pig farm in 2018. The majority of pig isolates harbor only one Shiga toxin gene, stx(2a), while 70% (35/50) of the cattle isolates have both stx(1a) and stx(2a). The results show some E. coli O157:H7 strains could establish persistence on pig farms and as such, pigs can be a significant source of the organism. Nature Publishing Group UK 2021-07-01 /pmc/articles/PMC8249597/ /pubmed/34210979 http://dx.doi.org/10.1038/s41538-021-00097-0 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhang, Peipei Essendoubi, Saida Keenliside, Julia Reuter, Tim Stanford, Kim King, Robin Lu, Patricia Yang, Xianqin Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments |
title | Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments |
title_full | Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments |
title_fullStr | Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments |
title_full_unstemmed | Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments |
title_short | Genomic analysis of Shiga toxin-producing Escherichia coli O157:H7 from cattle and pork-production related environments |
title_sort | genomic analysis of shiga toxin-producing escherichia coli o157:h7 from cattle and pork-production related environments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249597/ https://www.ncbi.nlm.nih.gov/pubmed/34210979 http://dx.doi.org/10.1038/s41538-021-00097-0 |
work_keys_str_mv | AT zhangpeipei genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments AT essendoubisaida genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments AT keenlisidejulia genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments AT reutertim genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments AT stanfordkim genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments AT kingrobin genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments AT lupatricia genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments AT yangxianqin genomicanalysisofshigatoxinproducingescherichiacolio157h7fromcattleandporkproductionrelatedenvironments |