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A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line

The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we hav...

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Autores principales: Soler, D. C., Kowatz, T., Sloan, A. E., McCormick, T. S., Cooper, K. D., Stepanyan, R., Engel, A., Vahedi-Faridi, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249660/
https://www.ncbi.nlm.nih.gov/pubmed/34211055
http://dx.doi.org/10.1038/s41598-021-93117-8
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author Soler, D. C.
Kowatz, T.
Sloan, A. E.
McCormick, T. S.
Cooper, K. D.
Stepanyan, R.
Engel, A.
Vahedi-Faridi, A.
author_facet Soler, D. C.
Kowatz, T.
Sloan, A. E.
McCormick, T. S.
Cooper, K. D.
Stepanyan, R.
Engel, A.
Vahedi-Faridi, A.
author_sort Soler, D. C.
collection PubMed
description The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma membrane expression. Serine substitution corroborated that amino acids present within AQP6(194–213) of AQP6 loop C contribute to intracellular endoplasmic reticulum (ER) retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous expression systems.
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spelling pubmed-82496602021-07-06 A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line Soler, D. C. Kowatz, T. Sloan, A. E. McCormick, T. S. Cooper, K. D. Stepanyan, R. Engel, A. Vahedi-Faridi, A. Sci Rep Article The inability to over-express Aquaporin 6 (AQP6) in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail using crystallographic approaches. Using an Aquaporin 3-tGFP Reporter (AGR) system we have identified a region within loop C of AQP6 that is responsible for severely hampering plasma membrane expression. Serine substitution corroborated that amino acids present within AQP6(194–213) of AQP6 loop C contribute to intracellular endoplasmic reticulum (ER) retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous expression systems. Nature Publishing Group UK 2021-07-01 /pmc/articles/PMC8249660/ /pubmed/34211055 http://dx.doi.org/10.1038/s41598-021-93117-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Soler, D. C.
Kowatz, T.
Sloan, A. E.
McCormick, T. S.
Cooper, K. D.
Stepanyan, R.
Engel, A.
Vahedi-Faridi, A.
A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_full A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_fullStr A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_full_unstemmed A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_short A region within the third extracellular loop of rat Aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
title_sort region within the third extracellular loop of rat aquaporin 6 precludes trafficking to plasma membrane in a heterologous cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249660/
https://www.ncbi.nlm.nih.gov/pubmed/34211055
http://dx.doi.org/10.1038/s41598-021-93117-8
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