Cargando…
Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars
Understanding the genetics of drought tolerance can expedite the development of drought-tolerant cultivars in wheat. In this study, we dissected the genetics of drought tolerance in spring wheat using a recombinant inbred line (RIL) population derived from a cross between a drought-tolerant cultivar...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249774/ https://www.ncbi.nlm.nih.gov/pubmed/34220939 http://dx.doi.org/10.3389/fgene.2021.656037 |
_version_ | 1783716968309719040 |
---|---|
author | Rabbi, S. M. Hisam Al Kumar, Ajay Mohajeri Naraghi, Sepehr Sapkota, Suraj Alamri, Mohammed S. Elias, Elias M. Kianian, Shahryar Seetan, Raed Missaoui, Ali Solanki, Shyam Mergoum, Mohamed |
author_facet | Rabbi, S. M. Hisam Al Kumar, Ajay Mohajeri Naraghi, Sepehr Sapkota, Suraj Alamri, Mohammed S. Elias, Elias M. Kianian, Shahryar Seetan, Raed Missaoui, Ali Solanki, Shyam Mergoum, Mohamed |
author_sort | Rabbi, S. M. Hisam Al |
collection | PubMed |
description | Understanding the genetics of drought tolerance can expedite the development of drought-tolerant cultivars in wheat. In this study, we dissected the genetics of drought tolerance in spring wheat using a recombinant inbred line (RIL) population derived from a cross between a drought-tolerant cultivar, ‘Reeder’ (PI613586), and a high-yielding but drought-susceptible cultivar, ‘Albany.’ The RIL population was evaluated for grain yield (YLD), grain volume weight (GVW), thousand kernel weight (TKW), plant height (PH), and days to heading (DH) at nine different environments. The Infinium 90 k-based high-density genetic map was generated using 10,657 polymorphic SNP markers representing 2,057 unique loci. Quantitative trait loci (QTL) analysis detected a total of 11 consistent QTL for drought tolerance-related traits. Of these, six QTL were exclusively identified in drought-prone environments, and five were constitutive QTL (identified under both drought and normal conditions). One major QTL on chromosome 7B was identified exclusively under drought environments and explained 13.6% of the phenotypic variation (PV) for YLD. Two other major QTL were detected, one each on chromosomes 7B and 2B under drought-prone environments, and explained 14.86 and 13.94% of phenotypic variation for GVW and YLD, respectively. One novel QTL for drought tolerance was identified on chromosome 2D. In silico expression analysis of candidate genes underlaying the exclusive QTLs associated with drought stress identified the enrichment of ribosomal and chloroplast photosynthesis-associated proteins showing the most expression variability, thus possibly contributing to stress response by modulating the glycosyltransferase (TraesCS6A01G116400) and hexosyltransferase (TraesCS7B01G013300) unique genes present in QTL 21 and 24, respectively. While both parents contributed favorable alleles to these QTL, unexpectedly, the high-yielding and less drought-tolerant parent contributed desirable alleles for drought tolerance at four out of six loci. Regardless of the origin, all QTL with significant drought tolerance could assist significantly in the development of drought-tolerant wheat cultivars, using genomics-assisted breeding approaches. |
format | Online Article Text |
id | pubmed-8249774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82497742021-07-03 Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars Rabbi, S. M. Hisam Al Kumar, Ajay Mohajeri Naraghi, Sepehr Sapkota, Suraj Alamri, Mohammed S. Elias, Elias M. Kianian, Shahryar Seetan, Raed Missaoui, Ali Solanki, Shyam Mergoum, Mohamed Front Genet Genetics Understanding the genetics of drought tolerance can expedite the development of drought-tolerant cultivars in wheat. In this study, we dissected the genetics of drought tolerance in spring wheat using a recombinant inbred line (RIL) population derived from a cross between a drought-tolerant cultivar, ‘Reeder’ (PI613586), and a high-yielding but drought-susceptible cultivar, ‘Albany.’ The RIL population was evaluated for grain yield (YLD), grain volume weight (GVW), thousand kernel weight (TKW), plant height (PH), and days to heading (DH) at nine different environments. The Infinium 90 k-based high-density genetic map was generated using 10,657 polymorphic SNP markers representing 2,057 unique loci. Quantitative trait loci (QTL) analysis detected a total of 11 consistent QTL for drought tolerance-related traits. Of these, six QTL were exclusively identified in drought-prone environments, and five were constitutive QTL (identified under both drought and normal conditions). One major QTL on chromosome 7B was identified exclusively under drought environments and explained 13.6% of the phenotypic variation (PV) for YLD. Two other major QTL were detected, one each on chromosomes 7B and 2B under drought-prone environments, and explained 14.86 and 13.94% of phenotypic variation for GVW and YLD, respectively. One novel QTL for drought tolerance was identified on chromosome 2D. In silico expression analysis of candidate genes underlaying the exclusive QTLs associated with drought stress identified the enrichment of ribosomal and chloroplast photosynthesis-associated proteins showing the most expression variability, thus possibly contributing to stress response by modulating the glycosyltransferase (TraesCS6A01G116400) and hexosyltransferase (TraesCS7B01G013300) unique genes present in QTL 21 and 24, respectively. While both parents contributed favorable alleles to these QTL, unexpectedly, the high-yielding and less drought-tolerant parent contributed desirable alleles for drought tolerance at four out of six loci. Regardless of the origin, all QTL with significant drought tolerance could assist significantly in the development of drought-tolerant wheat cultivars, using genomics-assisted breeding approaches. Frontiers Media S.A. 2021-06-17 /pmc/articles/PMC8249774/ /pubmed/34220939 http://dx.doi.org/10.3389/fgene.2021.656037 Text en Copyright © 2021 Rabbi, Kumar, Mohajeri Naraghi, Sapkota, Alamri, Elias, Kianian, Seetan, Missaoui, Solanki and Mergoum. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Rabbi, S. M. Hisam Al Kumar, Ajay Mohajeri Naraghi, Sepehr Sapkota, Suraj Alamri, Mohammed S. Elias, Elias M. Kianian, Shahryar Seetan, Raed Missaoui, Ali Solanki, Shyam Mergoum, Mohamed Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
title | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
title_full | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
title_fullStr | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
title_full_unstemmed | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
title_short | Identification of Main-Effect and Environmental Interaction QTL and Their Candidate Genes for Drought Tolerance in a Wheat RIL Population Between Two Elite Spring Cultivars |
title_sort | identification of main-effect and environmental interaction qtl and their candidate genes for drought tolerance in a wheat ril population between two elite spring cultivars |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249774/ https://www.ncbi.nlm.nih.gov/pubmed/34220939 http://dx.doi.org/10.3389/fgene.2021.656037 |
work_keys_str_mv | AT rabbismhisamal identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT kumarajay identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT mohajerinaraghisepehr identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT sapkotasuraj identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT alamrimohammeds identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT eliaseliasm identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT kianianshahryar identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT seetanraed identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT missaouiali identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT solankishyam identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars AT mergoummohamed identificationofmaineffectandenvironmentalinteractionqtlandtheircandidategenesfordroughttoleranceinawheatrilpopulationbetweentwoelitespringcultivars |