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Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)

Fusarium wilt is a destructive soil-borne disease that threatens the production of mung bean. Mung bean lines Zheng8-4 and Zheng8-20 show high resistance and high susceptibility to Fusarium wilt, respectively. Transcriptome analysis was carried out to identify candidate genes involved in Fusarium wi...

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Autores principales: Chang, Yujie, Sun, Feifei, Sun, Suli, Wang, Lanfen, Wu, Jing, Zhu, Zhendong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249807/
https://www.ncbi.nlm.nih.gov/pubmed/34220899
http://dx.doi.org/10.3389/fpls.2021.679629
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author Chang, Yujie
Sun, Feifei
Sun, Suli
Wang, Lanfen
Wu, Jing
Zhu, Zhendong
author_facet Chang, Yujie
Sun, Feifei
Sun, Suli
Wang, Lanfen
Wu, Jing
Zhu, Zhendong
author_sort Chang, Yujie
collection PubMed
description Fusarium wilt is a destructive soil-borne disease that threatens the production of mung bean. Mung bean lines Zheng8-4 and Zheng8-20 show high resistance and high susceptibility to Fusarium wilt, respectively. Transcriptome analysis was carried out to identify candidate genes involved in Fusarium wilt resistance using Zheng8-4 and Zheng8-20 at 0, 0.5, 1, 2, and 4 days post inoculation (dpi). Differential expression analysis showed that 3,254 genes responded to pathogen infection and were differentially expressed in the resistant and susceptible lines. Weighted gene co-expression network analysis (WGCNA) was also performed to identify five modules highly correlated with Fusarium wilt resistance, in which 453 differentially expressed genes (DEGs) were considered likely to be involved in Fusarium wilt resistance. Among these DEGs, we found 24 genes encoding resistance (R) proteins, 22 encoding protein kinases, 20 belonging to transcription factor families, 34 encoding proteins with oxidoreductase activity, 17 involved in stimulation/stress responses, and 54 annotated to pathogen resistance-related pathways. Finally, 27 annotated genes were further selected as candidate genes of Fusarium wilt resistance in mung bean. This study identifies novel potential resistance-related genes against Fusarium wilt and provides a theoretical basis for further investigation of Fusarium wilt resistance in mung bean breeding.
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spelling pubmed-82498072021-07-03 Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.) Chang, Yujie Sun, Feifei Sun, Suli Wang, Lanfen Wu, Jing Zhu, Zhendong Front Plant Sci Plant Science Fusarium wilt is a destructive soil-borne disease that threatens the production of mung bean. Mung bean lines Zheng8-4 and Zheng8-20 show high resistance and high susceptibility to Fusarium wilt, respectively. Transcriptome analysis was carried out to identify candidate genes involved in Fusarium wilt resistance using Zheng8-4 and Zheng8-20 at 0, 0.5, 1, 2, and 4 days post inoculation (dpi). Differential expression analysis showed that 3,254 genes responded to pathogen infection and were differentially expressed in the resistant and susceptible lines. Weighted gene co-expression network analysis (WGCNA) was also performed to identify five modules highly correlated with Fusarium wilt resistance, in which 453 differentially expressed genes (DEGs) were considered likely to be involved in Fusarium wilt resistance. Among these DEGs, we found 24 genes encoding resistance (R) proteins, 22 encoding protein kinases, 20 belonging to transcription factor families, 34 encoding proteins with oxidoreductase activity, 17 involved in stimulation/stress responses, and 54 annotated to pathogen resistance-related pathways. Finally, 27 annotated genes were further selected as candidate genes of Fusarium wilt resistance in mung bean. This study identifies novel potential resistance-related genes against Fusarium wilt and provides a theoretical basis for further investigation of Fusarium wilt resistance in mung bean breeding. Frontiers Media S.A. 2021-06-17 /pmc/articles/PMC8249807/ /pubmed/34220899 http://dx.doi.org/10.3389/fpls.2021.679629 Text en Copyright © 2021 Chang, Sun, Sun, Wang, Wu and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chang, Yujie
Sun, Feifei
Sun, Suli
Wang, Lanfen
Wu, Jing
Zhu, Zhendong
Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)
title Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)
title_full Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)
title_fullStr Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)
title_full_unstemmed Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)
title_short Transcriptome Analysis of Resistance to Fusarium Wilt in Mung Bean (Vigna radiata L.)
title_sort transcriptome analysis of resistance to fusarium wilt in mung bean (vigna radiata l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249807/
https://www.ncbi.nlm.nih.gov/pubmed/34220899
http://dx.doi.org/10.3389/fpls.2021.679629
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