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Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses

Durian is an economically important fruit of Southeast Asia. There is, however, a lack of in-depth information on the alteration of its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from the pulp of durian cv. Monthong fruit at unripe and ripe stages, usin...

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Autores principales: Sangpong, Lalida, Khaksar, Gholamreza, Pinsorn, Pinnapat, Oikawa, Akira, Sasaki, Ryosuke, Erban, Alexander, Watanabe, Mutsumi, Wangpaiboon, Karan, Tohge, Takayuki, Kopka, Joachim, Hoefgen, Rainer, Saito, Kazuki, Sirikantaramas, Supaart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250156/
https://www.ncbi.nlm.nih.gov/pubmed/34220909
http://dx.doi.org/10.3389/fpls.2021.687799
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author Sangpong, Lalida
Khaksar, Gholamreza
Pinsorn, Pinnapat
Oikawa, Akira
Sasaki, Ryosuke
Erban, Alexander
Watanabe, Mutsumi
Wangpaiboon, Karan
Tohge, Takayuki
Kopka, Joachim
Hoefgen, Rainer
Saito, Kazuki
Sirikantaramas, Supaart
author_facet Sangpong, Lalida
Khaksar, Gholamreza
Pinsorn, Pinnapat
Oikawa, Akira
Sasaki, Ryosuke
Erban, Alexander
Watanabe, Mutsumi
Wangpaiboon, Karan
Tohge, Takayuki
Kopka, Joachim
Hoefgen, Rainer
Saito, Kazuki
Sirikantaramas, Supaart
author_sort Sangpong, Lalida
collection PubMed
description Durian is an economically important fruit of Southeast Asia. There is, however, a lack of in-depth information on the alteration of its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from the pulp of durian cv. Monthong fruit at unripe and ripe stages, using capillary electrophoresis- and gas chromatography- time-of-flight mass spectrometry, specifically focusing on taste-related metabolites. During ripening, sucrose content increased. Change in raffinose-family oligosaccharides are reported herein for the first time. The malate and succinate contents increased, while those of citrate, an abundant organic acid, were unchanged. Notably, most amino acids increased, including isoleucine, leucine, and valine, whereas aspartate decreased, and glutamate was unchanged. Furthermore, transcriptomic analysis was performed to analyze the dynamic changes in sugar metabolism, glycolysis, TCA cycle, and amino acid pathways to identify key candidate genes. Taken together, our results elucidate the fundamental taste-related metabolism of durian, which can be exploited to develop durian metabolic and genetic markers in the future.
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spelling pubmed-82501562021-07-03 Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses Sangpong, Lalida Khaksar, Gholamreza Pinsorn, Pinnapat Oikawa, Akira Sasaki, Ryosuke Erban, Alexander Watanabe, Mutsumi Wangpaiboon, Karan Tohge, Takayuki Kopka, Joachim Hoefgen, Rainer Saito, Kazuki Sirikantaramas, Supaart Front Plant Sci Plant Science Durian is an economically important fruit of Southeast Asia. There is, however, a lack of in-depth information on the alteration of its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from the pulp of durian cv. Monthong fruit at unripe and ripe stages, using capillary electrophoresis- and gas chromatography- time-of-flight mass spectrometry, specifically focusing on taste-related metabolites. During ripening, sucrose content increased. Change in raffinose-family oligosaccharides are reported herein for the first time. The malate and succinate contents increased, while those of citrate, an abundant organic acid, were unchanged. Notably, most amino acids increased, including isoleucine, leucine, and valine, whereas aspartate decreased, and glutamate was unchanged. Furthermore, transcriptomic analysis was performed to analyze the dynamic changes in sugar metabolism, glycolysis, TCA cycle, and amino acid pathways to identify key candidate genes. Taken together, our results elucidate the fundamental taste-related metabolism of durian, which can be exploited to develop durian metabolic and genetic markers in the future. Frontiers Media S.A. 2021-06-18 /pmc/articles/PMC8250156/ /pubmed/34220909 http://dx.doi.org/10.3389/fpls.2021.687799 Text en Copyright © 2021 Sangpong, Khaksar, Pinsorn, Oikawa, Sasaki, Erban, Watanabe, Wangpaiboon, Tohge, Kopka, Hoefgen, Saito and Sirikantaramas. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sangpong, Lalida
Khaksar, Gholamreza
Pinsorn, Pinnapat
Oikawa, Akira
Sasaki, Ryosuke
Erban, Alexander
Watanabe, Mutsumi
Wangpaiboon, Karan
Tohge, Takayuki
Kopka, Joachim
Hoefgen, Rainer
Saito, Kazuki
Sirikantaramas, Supaart
Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses
title Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses
title_full Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses
title_fullStr Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses
title_full_unstemmed Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses
title_short Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses
title_sort assessing dynamic changes of taste-related primary metabolism during ripening of durian pulp using metabolomic and transcriptomic analyses
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250156/
https://www.ncbi.nlm.nih.gov/pubmed/34220909
http://dx.doi.org/10.3389/fpls.2021.687799
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