Cargando…
Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses
Durian is an economically important fruit of Southeast Asia. There is, however, a lack of in-depth information on the alteration of its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from the pulp of durian cv. Monthong fruit at unripe and ripe stages, usin...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250156/ https://www.ncbi.nlm.nih.gov/pubmed/34220909 http://dx.doi.org/10.3389/fpls.2021.687799 |
_version_ | 1783717031017709568 |
---|---|
author | Sangpong, Lalida Khaksar, Gholamreza Pinsorn, Pinnapat Oikawa, Akira Sasaki, Ryosuke Erban, Alexander Watanabe, Mutsumi Wangpaiboon, Karan Tohge, Takayuki Kopka, Joachim Hoefgen, Rainer Saito, Kazuki Sirikantaramas, Supaart |
author_facet | Sangpong, Lalida Khaksar, Gholamreza Pinsorn, Pinnapat Oikawa, Akira Sasaki, Ryosuke Erban, Alexander Watanabe, Mutsumi Wangpaiboon, Karan Tohge, Takayuki Kopka, Joachim Hoefgen, Rainer Saito, Kazuki Sirikantaramas, Supaart |
author_sort | Sangpong, Lalida |
collection | PubMed |
description | Durian is an economically important fruit of Southeast Asia. There is, however, a lack of in-depth information on the alteration of its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from the pulp of durian cv. Monthong fruit at unripe and ripe stages, using capillary electrophoresis- and gas chromatography- time-of-flight mass spectrometry, specifically focusing on taste-related metabolites. During ripening, sucrose content increased. Change in raffinose-family oligosaccharides are reported herein for the first time. The malate and succinate contents increased, while those of citrate, an abundant organic acid, were unchanged. Notably, most amino acids increased, including isoleucine, leucine, and valine, whereas aspartate decreased, and glutamate was unchanged. Furthermore, transcriptomic analysis was performed to analyze the dynamic changes in sugar metabolism, glycolysis, TCA cycle, and amino acid pathways to identify key candidate genes. Taken together, our results elucidate the fundamental taste-related metabolism of durian, which can be exploited to develop durian metabolic and genetic markers in the future. |
format | Online Article Text |
id | pubmed-8250156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82501562021-07-03 Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses Sangpong, Lalida Khaksar, Gholamreza Pinsorn, Pinnapat Oikawa, Akira Sasaki, Ryosuke Erban, Alexander Watanabe, Mutsumi Wangpaiboon, Karan Tohge, Takayuki Kopka, Joachim Hoefgen, Rainer Saito, Kazuki Sirikantaramas, Supaart Front Plant Sci Plant Science Durian is an economically important fruit of Southeast Asia. There is, however, a lack of in-depth information on the alteration of its metabolic networks during ripening. Here, we annotated 94 ripening-associated metabolites from the pulp of durian cv. Monthong fruit at unripe and ripe stages, using capillary electrophoresis- and gas chromatography- time-of-flight mass spectrometry, specifically focusing on taste-related metabolites. During ripening, sucrose content increased. Change in raffinose-family oligosaccharides are reported herein for the first time. The malate and succinate contents increased, while those of citrate, an abundant organic acid, were unchanged. Notably, most amino acids increased, including isoleucine, leucine, and valine, whereas aspartate decreased, and glutamate was unchanged. Furthermore, transcriptomic analysis was performed to analyze the dynamic changes in sugar metabolism, glycolysis, TCA cycle, and amino acid pathways to identify key candidate genes. Taken together, our results elucidate the fundamental taste-related metabolism of durian, which can be exploited to develop durian metabolic and genetic markers in the future. Frontiers Media S.A. 2021-06-18 /pmc/articles/PMC8250156/ /pubmed/34220909 http://dx.doi.org/10.3389/fpls.2021.687799 Text en Copyright © 2021 Sangpong, Khaksar, Pinsorn, Oikawa, Sasaki, Erban, Watanabe, Wangpaiboon, Tohge, Kopka, Hoefgen, Saito and Sirikantaramas. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Sangpong, Lalida Khaksar, Gholamreza Pinsorn, Pinnapat Oikawa, Akira Sasaki, Ryosuke Erban, Alexander Watanabe, Mutsumi Wangpaiboon, Karan Tohge, Takayuki Kopka, Joachim Hoefgen, Rainer Saito, Kazuki Sirikantaramas, Supaart Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses |
title | Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses |
title_full | Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses |
title_fullStr | Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses |
title_full_unstemmed | Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses |
title_short | Assessing Dynamic Changes of Taste-Related Primary Metabolism During Ripening of Durian Pulp Using Metabolomic and Transcriptomic Analyses |
title_sort | assessing dynamic changes of taste-related primary metabolism during ripening of durian pulp using metabolomic and transcriptomic analyses |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250156/ https://www.ncbi.nlm.nih.gov/pubmed/34220909 http://dx.doi.org/10.3389/fpls.2021.687799 |
work_keys_str_mv | AT sangponglalida assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT khaksargholamreza assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT pinsornpinnapat assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT oikawaakira assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT sasakiryosuke assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT erbanalexander assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT watanabemutsumi assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT wangpaiboonkaran assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT tohgetakayuki assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT kopkajoachim assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT hoefgenrainer assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT saitokazuki assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses AT sirikantaramassupaart assessingdynamicchangesoftasterelatedprimarymetabolismduringripeningofdurianpulpusingmetabolomicandtranscriptomicanalyses |