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Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition

Dental caries is one of the most prevalent chronic oral diseases, affecting approximately half of children worldwide. The microbial composition of dental caries may depend on age, oral health, diet, and geography, yet the effect of geography on these microbiomes is largely underexplored. Here, we pr...

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Autores principales: Li, Shanshan, Huang, Shi, Guo, Yi, Zhang, Ying, Zhang, Lijuan, Li, Fan, Tan, Kaixuan, Lu, Jie, Chen, Zhenggang, Guo, Qingyuan, Tang, Yongping, Teng, Fei, Yang, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250437/
https://www.ncbi.nlm.nih.gov/pubmed/34222048
http://dx.doi.org/10.3389/fcimb.2021.680288
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author Li, Shanshan
Huang, Shi
Guo, Yi
Zhang, Ying
Zhang, Lijuan
Li, Fan
Tan, Kaixuan
Lu, Jie
Chen, Zhenggang
Guo, Qingyuan
Tang, Yongping
Teng, Fei
Yang, Fang
author_facet Li, Shanshan
Huang, Shi
Guo, Yi
Zhang, Ying
Zhang, Lijuan
Li, Fan
Tan, Kaixuan
Lu, Jie
Chen, Zhenggang
Guo, Qingyuan
Tang, Yongping
Teng, Fei
Yang, Fang
author_sort Li, Shanshan
collection PubMed
description Dental caries is one of the most prevalent chronic oral diseases, affecting approximately half of children worldwide. The microbial composition of dental caries may depend on age, oral health, diet, and geography, yet the effect of geography on these microbiomes is largely underexplored. Here, we profiled and compared saliva microbiota from 130 individuals aged 6 to 8 years old, representing both healthy children (H group) and children with caries-affected (C group) from two geographical regions of China: a northern city (Qingdao group) and a southern city (Guangzhou group). First, the saliva microbiota exhibited profound differences in diversity and composition between the C and H groups. The caries microbiota featured a lower alpha diversity and more variable community structure than the healthy microbiota. Furthermore, the relative abundance of several genera (e.g., Lactobacillus, Gemella, Cryptobacterium and Mitsuokella) was significantly higher in the C group than in the H group (p<0.05). Next, geography dominated over disease status in shaping salivary microbiota, and a wide array of salivary bacteria was highly predictive of the individuals’ city of origin. Finally, we built a universal diagnostic model based on 14 bacterial species, which can diagnose caries with 87% (AUC=86.00%) and 85% (AUC=91.02%) accuracy within each city and 83% accuracy across cities (AUC=92.17%). Although the detection rate of Streptococcus mutans in populations is not very high, it could be regarded as a single biomarker to diagnose caries with decent accuracy. These findings demonstrated that despite the large effect size of geography, a universal model based on salivary microbiota has the potential to diagnose caries across the Chinese child population.
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spelling pubmed-82504372021-07-03 Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition Li, Shanshan Huang, Shi Guo, Yi Zhang, Ying Zhang, Lijuan Li, Fan Tan, Kaixuan Lu, Jie Chen, Zhenggang Guo, Qingyuan Tang, Yongping Teng, Fei Yang, Fang Front Cell Infect Microbiol Cellular and Infection Microbiology Dental caries is one of the most prevalent chronic oral diseases, affecting approximately half of children worldwide. The microbial composition of dental caries may depend on age, oral health, diet, and geography, yet the effect of geography on these microbiomes is largely underexplored. Here, we profiled and compared saliva microbiota from 130 individuals aged 6 to 8 years old, representing both healthy children (H group) and children with caries-affected (C group) from two geographical regions of China: a northern city (Qingdao group) and a southern city (Guangzhou group). First, the saliva microbiota exhibited profound differences in diversity and composition between the C and H groups. The caries microbiota featured a lower alpha diversity and more variable community structure than the healthy microbiota. Furthermore, the relative abundance of several genera (e.g., Lactobacillus, Gemella, Cryptobacterium and Mitsuokella) was significantly higher in the C group than in the H group (p<0.05). Next, geography dominated over disease status in shaping salivary microbiota, and a wide array of salivary bacteria was highly predictive of the individuals’ city of origin. Finally, we built a universal diagnostic model based on 14 bacterial species, which can diagnose caries with 87% (AUC=86.00%) and 85% (AUC=91.02%) accuracy within each city and 83% accuracy across cities (AUC=92.17%). Although the detection rate of Streptococcus mutans in populations is not very high, it could be regarded as a single biomarker to diagnose caries with decent accuracy. These findings demonstrated that despite the large effect size of geography, a universal model based on salivary microbiota has the potential to diagnose caries across the Chinese child population. Frontiers Media S.A. 2021-06-18 /pmc/articles/PMC8250437/ /pubmed/34222048 http://dx.doi.org/10.3389/fcimb.2021.680288 Text en Copyright © 2021 Li, Huang, Guo, Zhang, Zhang, Li, Tan, Lu, Chen, Guo, Tang, Teng and Yang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Li, Shanshan
Huang, Shi
Guo, Yi
Zhang, Ying
Zhang, Lijuan
Li, Fan
Tan, Kaixuan
Lu, Jie
Chen, Zhenggang
Guo, Qingyuan
Tang, Yongping
Teng, Fei
Yang, Fang
Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition
title Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition
title_full Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition
title_fullStr Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition
title_full_unstemmed Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition
title_short Geographic Variation Did Not Affect the Predictive Power of Salivary Microbiota for Caries in Children With Mixed Dentition
title_sort geographic variation did not affect the predictive power of salivary microbiota for caries in children with mixed dentition
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8250437/
https://www.ncbi.nlm.nih.gov/pubmed/34222048
http://dx.doi.org/10.3389/fcimb.2021.680288
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