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Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps

AIMS: Assess the feasibility of using light from artificial sun lamps to decontaminate N95 filtering facepiece respirators (FFRs) contaminated with SARS‐CoV‐2. METHODS AND RESULTS: FFR coupons or whole FFRs contaminated with 5 log(10) TCID(50) (target concentration) SARS‐CoV‐2 in culture media, simu...

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Autores principales: Glasbrenner, D.C., Choi, Y.W., Richardson, A.W., Edwards, E.W., Mladineo, M.J., Sunderman, M., Keyes, P.H., Boyce, J., Middleton, J.K., Howard, M.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Science Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251224/
https://www.ncbi.nlm.nih.gov/pubmed/33884721
http://dx.doi.org/10.1111/jam.15106
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author Glasbrenner, D.C.
Choi, Y.W.
Richardson, A.W.
Edwards, E.W.
Mladineo, M.J.
Sunderman, M.
Keyes, P.H.
Boyce, J.
Middleton, J.K.
Howard, M.W.
author_facet Glasbrenner, D.C.
Choi, Y.W.
Richardson, A.W.
Edwards, E.W.
Mladineo, M.J.
Sunderman, M.
Keyes, P.H.
Boyce, J.
Middleton, J.K.
Howard, M.W.
author_sort Glasbrenner, D.C.
collection PubMed
description AIMS: Assess the feasibility of using light from artificial sun lamps to decontaminate N95 filtering facepiece respirators (FFRs) contaminated with SARS‐CoV‐2. METHODS AND RESULTS: FFR coupons or whole FFRs contaminated with 5 log(10) TCID(50) (target concentration) SARS‐CoV‐2 in culture media, simulated saliva, or simulated lung fluid were dried for 1–2 h, then exposed to light from tanning and horticulture lamps to assess decontamination. Exposed coupons and whole FFRs showed SARS‐CoV‐2 inactivation for all matrices tested. Furthermore, FFRs still met performance specifications after five decontamination cycles. CONCLUSIONS: It is feasible that artificial sunlight from these sun lamps can be used to decontaminate FFRs provided the UV dose is sufficient and the light is unobstructed. Furthermore, decontamination can be performed up to five times without degrading FFR performance. SIGNIFICANCE AND IMPACT OF THE STUDY: This research shows a proof of principle that artificial sun lamps may be an option to decontaminate SARS‐CoV‐2 on N95 FFRs. UV doses required for inactivation to levels below detection ranged from 4 to 37·8 J cm(−2) depending on the light source, virus matrix and FFR type.
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spelling pubmed-82512242021-07-02 Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps Glasbrenner, D.C. Choi, Y.W. Richardson, A.W. Edwards, E.W. Mladineo, M.J. Sunderman, M. Keyes, P.H. Boyce, J. Middleton, J.K. Howard, M.W. J Appl Microbiol Original Articles AIMS: Assess the feasibility of using light from artificial sun lamps to decontaminate N95 filtering facepiece respirators (FFRs) contaminated with SARS‐CoV‐2. METHODS AND RESULTS: FFR coupons or whole FFRs contaminated with 5 log(10) TCID(50) (target concentration) SARS‐CoV‐2 in culture media, simulated saliva, or simulated lung fluid were dried for 1–2 h, then exposed to light from tanning and horticulture lamps to assess decontamination. Exposed coupons and whole FFRs showed SARS‐CoV‐2 inactivation for all matrices tested. Furthermore, FFRs still met performance specifications after five decontamination cycles. CONCLUSIONS: It is feasible that artificial sunlight from these sun lamps can be used to decontaminate FFRs provided the UV dose is sufficient and the light is unobstructed. Furthermore, decontamination can be performed up to five times without degrading FFR performance. SIGNIFICANCE AND IMPACT OF THE STUDY: This research shows a proof of principle that artificial sun lamps may be an option to decontaminate SARS‐CoV‐2 on N95 FFRs. UV doses required for inactivation to levels below detection ranged from 4 to 37·8 J cm(−2) depending on the light source, virus matrix and FFR type. Blackwell Science Ltd 2021-11-01 /pmc/articles/PMC8251224/ /pubmed/33884721 http://dx.doi.org/10.1111/jam.15106 Text en © 2021 The Society for Applied Microbiology https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Original Articles
Glasbrenner, D.C.
Choi, Y.W.
Richardson, A.W.
Edwards, E.W.
Mladineo, M.J.
Sunderman, M.
Keyes, P.H.
Boyce, J.
Middleton, J.K.
Howard, M.W.
Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps
title Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps
title_full Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps
title_fullStr Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps
title_full_unstemmed Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps
title_short Decontamination of SARS‐CoV‐2 contaminated N95 filtering facepiece respirators using artificial sun lamps
title_sort decontamination of sars‐cov‐2 contaminated n95 filtering facepiece respirators using artificial sun lamps
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251224/
https://www.ncbi.nlm.nih.gov/pubmed/33884721
http://dx.doi.org/10.1111/jam.15106
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