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Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs
2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent‐driven exchanges to enhance NOE correlations between exchangeable and non‐exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities w...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251384/ https://www.ncbi.nlm.nih.gov/pubmed/33683819 http://dx.doi.org/10.1002/anie.202015948 |
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author | Novakovic, Mihajlo Kupče, Ēriks Scherf, Tali Oxenfarth, Andreas Schnieders, Robbin Grün, J. Tassilo Wirmer‐Bartoschek, Julia Richter, Christian Schwalbe, Harald Frydman, Lucio |
author_facet | Novakovic, Mihajlo Kupče, Ēriks Scherf, Tali Oxenfarth, Andreas Schnieders, Robbin Grün, J. Tassilo Wirmer‐Bartoschek, Julia Richter, Christian Schwalbe, Harald Frydman, Lucio |
author_sort | Novakovic, Mihajlo |
collection | PubMed |
description | 2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent‐driven exchanges to enhance NOE correlations between exchangeable and non‐exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino–imino cross‐peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS‐CoV‐2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts. |
format | Online Article Text |
id | pubmed-8251384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82513842021-07-02 Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs Novakovic, Mihajlo Kupče, Ēriks Scherf, Tali Oxenfarth, Andreas Schnieders, Robbin Grün, J. Tassilo Wirmer‐Bartoschek, Julia Richter, Christian Schwalbe, Harald Frydman, Lucio Angew Chem Int Ed Engl Research Articles 2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent‐driven exchanges to enhance NOE correlations between exchangeable and non‐exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino–imino cross‐peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS‐CoV‐2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts. John Wiley and Sons Inc. 2021-05-04 2021-05-17 /pmc/articles/PMC8251384/ /pubmed/33683819 http://dx.doi.org/10.1002/anie.202015948 Text en © 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Novakovic, Mihajlo Kupče, Ēriks Scherf, Tali Oxenfarth, Andreas Schnieders, Robbin Grün, J. Tassilo Wirmer‐Bartoschek, Julia Richter, Christian Schwalbe, Harald Frydman, Lucio Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs |
title | Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs |
title_full | Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs |
title_fullStr | Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs |
title_full_unstemmed | Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs |
title_short | Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs |
title_sort | magnetization transfer to enhance noe cross‐peaks among labile protons: applications to imino–imino sequential walks in sars‐cov‐2‐derived rnas |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251384/ https://www.ncbi.nlm.nih.gov/pubmed/33683819 http://dx.doi.org/10.1002/anie.202015948 |
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