Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis

3‐Hydroxyisoquinolines (ISOs) and their tautomeric isoquinolin‐3‐ones are heterocycles with attractive biological properties. Here we reported the revisited synthesis of a highly functionalized ISO that showed blue fluorescence and the characterization of its biological properties in an invertebrate...

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Autores principales: Mercurio, Silvia, Moni, Lisa, Scarì, Giorgio, Manenti, Raoul, Riva, Renata, Pennati, Roberta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251550/
https://www.ncbi.nlm.nih.gov/pubmed/33871133
http://dx.doi.org/10.1002/cbic.202100058
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author Mercurio, Silvia
Moni, Lisa
Scarì, Giorgio
Manenti, Raoul
Riva, Renata
Pennati, Roberta
author_facet Mercurio, Silvia
Moni, Lisa
Scarì, Giorgio
Manenti, Raoul
Riva, Renata
Pennati, Roberta
author_sort Mercurio, Silvia
collection PubMed
description 3‐Hydroxyisoquinolines (ISOs) and their tautomeric isoquinolin‐3‐ones are heterocycles with attractive biological properties. Here we reported the revisited synthesis of a highly functionalized ISO that showed blue fluorescence and the characterization of its biological properties in an invertebrate animal model, the ascidian Ciona intestinalis. Larvae exposed to ISO at concentrations higher than 1 μM showed an intense fluorescence localized in the cell nuclei of all tissues. Moreover, exposure to ISO interfered with larval ability to swim; this neuromuscular effect was reversible. Overall, these results suggested that ISOs can have promising applications as novel fluorescent dyes of the cell nuclei.
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spelling pubmed-82515502021-07-06 Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis Mercurio, Silvia Moni, Lisa Scarì, Giorgio Manenti, Raoul Riva, Renata Pennati, Roberta Chembiochem Full Papers 3‐Hydroxyisoquinolines (ISOs) and their tautomeric isoquinolin‐3‐ones are heterocycles with attractive biological properties. Here we reported the revisited synthesis of a highly functionalized ISO that showed blue fluorescence and the characterization of its biological properties in an invertebrate animal model, the ascidian Ciona intestinalis. Larvae exposed to ISO at concentrations higher than 1 μM showed an intense fluorescence localized in the cell nuclei of all tissues. Moreover, exposure to ISO interfered with larval ability to swim; this neuromuscular effect was reversible. Overall, these results suggested that ISOs can have promising applications as novel fluorescent dyes of the cell nuclei. John Wiley and Sons Inc. 2021-05-04 2021-06-15 /pmc/articles/PMC8251550/ /pubmed/33871133 http://dx.doi.org/10.1002/cbic.202100058 Text en © 2021 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Mercurio, Silvia
Moni, Lisa
Scarì, Giorgio
Manenti, Raoul
Riva, Renata
Pennati, Roberta
Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis
title Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis
title_full Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis
title_fullStr Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis
title_full_unstemmed Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis
title_short Fluorescence Properties of a Novel Isoquinoline Derivative Tested in an Invertebrate Chordate, Ciona intestinalis
title_sort fluorescence properties of a novel isoquinoline derivative tested in an invertebrate chordate, ciona intestinalis
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251550/
https://www.ncbi.nlm.nih.gov/pubmed/33871133
http://dx.doi.org/10.1002/cbic.202100058
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