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In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin

Human anamorsin is an iron–sulfur (Fe–S)‐cluster‐binding protein acting as an electron donor in the early steps of cytosolic iron–sulfur protein biogenesis. Human anamorsin belongs to the eukaryotic CIAPIN1 protein family and contains two highly conserved cysteine‐rich motifs, each binding an Fe–S c...

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Autores principales: Matteucci, Sara, Camponeschi, Francesca, Clémancey, Martin, Ciofi‐Baffoni, Simone, Blondin, Geneviève, Banci, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251831/
https://www.ncbi.nlm.nih.gov/pubmed/33852169
http://dx.doi.org/10.1002/anie.202102910
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author Matteucci, Sara
Camponeschi, Francesca
Clémancey, Martin
Ciofi‐Baffoni, Simone
Blondin, Geneviève
Banci, Lucia
author_facet Matteucci, Sara
Camponeschi, Francesca
Clémancey, Martin
Ciofi‐Baffoni, Simone
Blondin, Geneviève
Banci, Lucia
author_sort Matteucci, Sara
collection PubMed
description Human anamorsin is an iron–sulfur (Fe–S)‐cluster‐binding protein acting as an electron donor in the early steps of cytosolic iron–sulfur protein biogenesis. Human anamorsin belongs to the eukaryotic CIAPIN1 protein family and contains two highly conserved cysteine‐rich motifs, each binding an Fe–S cluster. In vitro works by various groups have provided rather controversial results for the type of Fe–S clusters bound to the CIAPIN1 proteins. In order to unravel the knot on this topic, we used an in cellulo approach combining Mössbauer and EPR spectroscopies to characterize the iron–sulfur‐cluster‐bound form of human anamorsin. We found that the protein binds two [2Fe–2S] clusters at both its cysteine‐rich motifs.
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spelling pubmed-82518312021-07-07 In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin Matteucci, Sara Camponeschi, Francesca Clémancey, Martin Ciofi‐Baffoni, Simone Blondin, Geneviève Banci, Lucia Angew Chem Int Ed Engl Communications Human anamorsin is an iron–sulfur (Fe–S)‐cluster‐binding protein acting as an electron donor in the early steps of cytosolic iron–sulfur protein biogenesis. Human anamorsin belongs to the eukaryotic CIAPIN1 protein family and contains two highly conserved cysteine‐rich motifs, each binding an Fe–S cluster. In vitro works by various groups have provided rather controversial results for the type of Fe–S clusters bound to the CIAPIN1 proteins. In order to unravel the knot on this topic, we used an in cellulo approach combining Mössbauer and EPR spectroscopies to characterize the iron–sulfur‐cluster‐bound form of human anamorsin. We found that the protein binds two [2Fe–2S] clusters at both its cysteine‐rich motifs. John Wiley and Sons Inc. 2021-05-24 2021-06-25 /pmc/articles/PMC8251831/ /pubmed/33852169 http://dx.doi.org/10.1002/anie.202102910 Text en © 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Matteucci, Sara
Camponeschi, Francesca
Clémancey, Martin
Ciofi‐Baffoni, Simone
Blondin, Geneviève
Banci, Lucia
In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin
title In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin
title_full In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin
title_fullStr In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin
title_full_unstemmed In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin
title_short In Cellulo Mössbauer and EPR Studies Bring New Evidence to the Long‐Standing Debate on Iron–Sulfur Cluster Binding in Human Anamorsin
title_sort in cellulo mössbauer and epr studies bring new evidence to the long‐standing debate on iron–sulfur cluster binding in human anamorsin
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251831/
https://www.ncbi.nlm.nih.gov/pubmed/33852169
http://dx.doi.org/10.1002/anie.202102910
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