Cargando…

Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR‐associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR‐Cas strategies in plants rely on genetic transformation using Agrobacterium tumefaciens to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Uranga, Mireia, Aragonés, Verónica, Selma, Sara, Vázquez‐Vilar, Marta, Orzáez, Diego, Daròs, José‐Antonio
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Technical Advance
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8251967/ https://www.ncbi.nlm.nih.gov/pubmed/33484202 http://dx.doi.org/10.1111/tpj.15164

Ejemplares similares

Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs
por: Zhang, Ju-Qing, et al.
Publicado: (2022)

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA
por: Jia, Hongge, et al.
Publicado: (2014)

Editing Citrus Genome via SaCas9/sgRNA System
por: Jia, Hongge, et al.
Publicado: (2017)

Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors
por: Uranga, Mireia, et al.
Publicado: (2023)

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
por: Long, Lu, et al.
Publicado: (2018)

Optimized paired‐sgRNA/Cas9 cloning and expression cassette triggers high‐efficiency multiplex genome editing in kiwifruit
por: Wang, Zupeng, et al.
Publicado: (2018)

Strong and tunable anti‐CRISPR/Cas activities in plants
por: Calvache, Camilo, et al.
Publicado: (2021)

sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing
por: Graf, Robin, et al.
Publicado: (2019)

Synthesis of an arrayed sgRNA library targeting the human genome
por: Schmidt, Tobias, et al.
Publicado: (2015)

Evaluation of CRISPR/Cas9 site-specific function and validation of sgRNA sequence by a Cas9/sgRNA-assisted reverse PCR technique
por: Zhang, Beibei, et al.
Publicado: (2021)

Novel CD123 polyaptamer hydrogel edited by Cas9/sgRNA for AML-targeted therapy
por: Wu, Haibin, et al.
Publicado: (2021)

EpiCas-DL: Predicting sgRNA activity for CRISPR-mediated epigenome editing by deep learning
por: Yang, Qianqian, et al.
Publicado: (2022)

Multiplexed Genome Editing via an RNA Polymerase II Promoter-Driven sgRNA Array in the Diatom Phaeodactylum tricornutum: Insights Into the Role of StLDP
por: Taparia, Yogesh, et al.
Publicado: (2022)

Multiplex CRISPR/Cas9-based genome engineering enhanced by Drosha-mediated sgRNA-shRNA structure
por: Yan, Qiang, et al.
Publicado: (2016)

Potato virus X‐delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming in Nicotiana benthamiana
por: Selma, Sara, et al.
Publicado: (2022)

Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
por: Dang, Ying, et al.
Publicado: (2015)

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins
por: Mangeot, Philippe E., et al.
Publicado: (2019)

Prediction of sgRNA Off-Target Activity in CRISPR/Cas9 Gene Editing Using Graph Convolution Network
por: Vinodkumar, Prasoon Kumar, et al.
Publicado: (2021)

Genome editing by introduction of Cas9/sgRNA into plant cells using temperature-controlled atmospheric pressure plasma
por: Yanagawa, Yuki, et al.
Publicado: (2023)

Design of a generic CRISPR-Cas9 approach using the same sgRNA to perform gene editing at distinct loci
por: Najah, Soumaya, et al.
Publicado: (2019)

Clinically Relevant Correction of Recessive Dystrophic Epidermolysis Bullosa by Dual sgRNA CRISPR/Cas9-Mediated Gene Editing
por: Bonafont, Jose, et al.
Publicado: (2019)

Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient ‘hit-and-run’ genome editing
por: Lyu, Pin, et al.
Publicado: (2019)

Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass
por: Kumar, Rahul, et al.
Publicado: (2022)

A Cas12a‐based gene editing system for Phytophthora infestans reveals monoallelic expression of an elicitor
por: Ah‐Fong, Audrey M.V., et al.
Publicado: (2021)

Target residence of Cas9-sgRNA influences DNA double-strand break repair pathway choices in CRISPR/Cas9 genome editing
por: Liu, Si-Cheng, et al.
Publicado: (2022)

CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries
por: Becker, Martin, et al.
Publicado: (2020)

Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice
por: Wu, Ying, et al.
Publicado: (2019)

sgRNA-shRNA Structure Mediated SNP Site Editing on Porcine IGF2 Gene by CRISPR/StCas9
por: Sun, Yongsen, et al.
Publicado: (2019)

CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations
por: Fukushima, Tsuyoshi, et al.
Publicado: (2021)

Miniature CRISPR-Cas12f1-Mediated Single-Nucleotide Microbial Genome Editing Using 3′-Truncated sgRNA
por: Lee, Ho Joung, et al.
Publicado: (2023)

CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
por: Chylinski, Krzysztof, et al.
Publicado: (2019)

Highly efficient genome editing in Xanthomonas oryzae pv. oryzae through repurposing the endogenous type I‐C CRISPR‐Cas system
por: Jiang, Dandan, et al.
Publicado: (2021)

Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins
por: Cho, Seung Woo, et al.
Publicado: (2013)

Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
por: Wang, Yong, et al.
Publicado: (2015)

Targeting N-Terminal Huntingtin with a Dual-sgRNA Strategy by CRISPR/Cas9
por: Wu, Junjiao, et al.
Publicado: (2019)

A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries
por: Yates, Joshua D, et al.
Publicado: (2021)

Full-Length Model of SaCas9-sgRNA-DNA Complex in Cleavage State
por: Du, Wenhao, et al.
Publicado: (2023)

sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine
por: Wang, Yanhong, et al.
Publicado: (2020)

Sequence determinants of improved CRISPR sgRNA design
por: Xu, Han, et al.
Publicado: (2015)

CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus
por: Yang, Hong, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_m7dtalh6433r3gjluv9nr9htg6