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Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy

In mammalian cochlea, sound‐induced vibration is amplified by a three‐row lattice of Y‐shaped microstructures consisting of electromotile outer hair cell and supporting Deiters cell. This highly organized structure is thought to be essential for hearing of low‐level sounds. Prior studies reported di...

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Autores principales: Wang, Haoyu, Wang, Shengxiong, Lu, Yan, Chen, Ying, Huang, Wenqing, Qiu, Miaoxin, Wu, Hao, Hua, Yunfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252425/
https://www.ncbi.nlm.nih.gov/pubmed/33719053
http://dx.doi.org/10.1002/cne.25137
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author Wang, Haoyu
Wang, Shengxiong
Lu, Yan
Chen, Ying
Huang, Wenqing
Qiu, Miaoxin
Wu, Hao
Hua, Yunfeng
author_facet Wang, Haoyu
Wang, Shengxiong
Lu, Yan
Chen, Ying
Huang, Wenqing
Qiu, Miaoxin
Wu, Hao
Hua, Yunfeng
author_sort Wang, Haoyu
collection PubMed
description In mammalian cochlea, sound‐induced vibration is amplified by a three‐row lattice of Y‐shaped microstructures consisting of electromotile outer hair cell and supporting Deiters cell. This highly organized structure is thought to be essential for hearing of low‐level sounds. Prior studies reported differences in geometry and synaptic innervation of the outer hair cells between rows, but how these fine features are achieved at subcellular level still remains unclear. Using serial block‐face electron microscopy, we acquired few‐hundred‐micron‐sized cytoarchitecture of mouse organ of Corti at nanometer resolution. Structural quantifications were performed on the Y‐shapes as well as afferent and efferent projections to outer hair cells (OHCs). Several new features, which support the previously observed inter‐row heterogeneity, are described. Our result provides structural bases for the gradient of mechanical properties and diverse centrifugal regulation of OHC rows.
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spelling pubmed-82524252021-07-07 Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy Wang, Haoyu Wang, Shengxiong Lu, Yan Chen, Ying Huang, Wenqing Qiu, Miaoxin Wu, Hao Hua, Yunfeng J Comp Neurol Research Articles In mammalian cochlea, sound‐induced vibration is amplified by a three‐row lattice of Y‐shaped microstructures consisting of electromotile outer hair cell and supporting Deiters cell. This highly organized structure is thought to be essential for hearing of low‐level sounds. Prior studies reported differences in geometry and synaptic innervation of the outer hair cells between rows, but how these fine features are achieved at subcellular level still remains unclear. Using serial block‐face electron microscopy, we acquired few‐hundred‐micron‐sized cytoarchitecture of mouse organ of Corti at nanometer resolution. Structural quantifications were performed on the Y‐shapes as well as afferent and efferent projections to outer hair cells (OHCs). Several new features, which support the previously observed inter‐row heterogeneity, are described. Our result provides structural bases for the gradient of mechanical properties and diverse centrifugal regulation of OHC rows. John Wiley & Sons, Inc. 2021-03-18 2021-08-01 /pmc/articles/PMC8252425/ /pubmed/33719053 http://dx.doi.org/10.1002/cne.25137 Text en © 2021 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Wang, Haoyu
Wang, Shengxiong
Lu, Yan
Chen, Ying
Huang, Wenqing
Qiu, Miaoxin
Wu, Hao
Hua, Yunfeng
Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy
title Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy
title_full Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy
title_fullStr Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy
title_full_unstemmed Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy
title_short Cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy
title_sort cytoarchitecture and innervation of the mouse cochlear amplifier revealed by large‐scale volume electron microscopy
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252425/
https://www.ncbi.nlm.nih.gov/pubmed/33719053
http://dx.doi.org/10.1002/cne.25137
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