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Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry

Resveratrol is a natural polyphenol compound produced in response to biotic and abiotic stresses in grape berries. However, changes in resveratrol caused by bud sport in grapes are scarcely reported. In this study, trans-resveratrol and cis-resveratrol were identified and quantified in the grape ber...

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Autores principales: Leng, Feng, Ye, Yunling, Zhou, Jialing, Jia, Huijuan, Zhu, Xiaoheng, Shi, Jiayu, Zhang, Ziyue, Shen, Nan, Wang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253253/
https://www.ncbi.nlm.nih.gov/pubmed/34220913
http://dx.doi.org/10.3389/fpls.2021.690095
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author Leng, Feng
Ye, Yunling
Zhou, Jialing
Jia, Huijuan
Zhu, Xiaoheng
Shi, Jiayu
Zhang, Ziyue
Shen, Nan
Wang, Li
author_facet Leng, Feng
Ye, Yunling
Zhou, Jialing
Jia, Huijuan
Zhu, Xiaoheng
Shi, Jiayu
Zhang, Ziyue
Shen, Nan
Wang, Li
author_sort Leng, Feng
collection PubMed
description Resveratrol is a natural polyphenol compound produced in response to biotic and abiotic stresses in grape berries. However, changes in resveratrol caused by bud sport in grapes are scarcely reported. In this study, trans-resveratrol and cis-resveratrol were identified and quantified in the grape berries of ‘Summer Black’ and its bud sport ‘Nantaihutezao’ from the veraison to ripening stages using ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS). We found that bud sport accumulates the trans-resveratrol earlier and increases the contents of cis-resveratrol in the earlier stages but decreases its contents in the later stages. Simultaneously, we used RNA-Seq to identify 51 transcripts involved in the stilbene pathways. In particular, we further identified 124 and 19 transcripts that negatively correlated with the contents of trans-resveratrol and cis-resveratrol, respectively, and four transcripts encoding F3'5'H that positively correlated with the contents of trans-resveratrol by weighted gene co-expression network analysis (WGCNA). These transcripts may play important roles in relation to the synergistic regulation of metabolisms of resveratrol. The results of this study can provide a theoretical basis for the genetic improvement of grapes.
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spelling pubmed-82532532021-07-03 Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry Leng, Feng Ye, Yunling Zhou, Jialing Jia, Huijuan Zhu, Xiaoheng Shi, Jiayu Zhang, Ziyue Shen, Nan Wang, Li Front Plant Sci Plant Science Resveratrol is a natural polyphenol compound produced in response to biotic and abiotic stresses in grape berries. However, changes in resveratrol caused by bud sport in grapes are scarcely reported. In this study, trans-resveratrol and cis-resveratrol were identified and quantified in the grape berries of ‘Summer Black’ and its bud sport ‘Nantaihutezao’ from the veraison to ripening stages using ultra performance liquid chromatography-high resolution tandem mass spectrometry (UPLC-HRMS). We found that bud sport accumulates the trans-resveratrol earlier and increases the contents of cis-resveratrol in the earlier stages but decreases its contents in the later stages. Simultaneously, we used RNA-Seq to identify 51 transcripts involved in the stilbene pathways. In particular, we further identified 124 and 19 transcripts that negatively correlated with the contents of trans-resveratrol and cis-resveratrol, respectively, and four transcripts encoding F3'5'H that positively correlated with the contents of trans-resveratrol by weighted gene co-expression network analysis (WGCNA). These transcripts may play important roles in relation to the synergistic regulation of metabolisms of resveratrol. The results of this study can provide a theoretical basis for the genetic improvement of grapes. Frontiers Media S.A. 2021-06-18 /pmc/articles/PMC8253253/ /pubmed/34220913 http://dx.doi.org/10.3389/fpls.2021.690095 Text en Copyright © 2021 Leng, Ye, Zhou, Jia, Zhu, Shi, Zhang, Shen and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Leng, Feng
Ye, Yunling
Zhou, Jialing
Jia, Huijuan
Zhu, Xiaoheng
Shi, Jiayu
Zhang, Ziyue
Shen, Nan
Wang, Li
Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry
title Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry
title_full Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry
title_fullStr Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry
title_full_unstemmed Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry
title_short Transcriptomic and Weighted Gene Co-expression Correlation Network Analysis Reveal Resveratrol Biosynthesis Mechanisms Caused by Bud Sport in Grape Berry
title_sort transcriptomic and weighted gene co-expression correlation network analysis reveal resveratrol biosynthesis mechanisms caused by bud sport in grape berry
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253253/
https://www.ncbi.nlm.nih.gov/pubmed/34220913
http://dx.doi.org/10.3389/fpls.2021.690095
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