Cargando…

Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum

Bacillus amyloliquefaciens YN201732 is an endophytic bacteria with high biocontrol efficiency and broad-spectrum antimicrobial activities. In order to clarify the main active ingredients and their antifungal mechanisms against powdery mildew of tobacco, this study is focused on lipopeptide obtained...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiao, Rong, Cai, Yongzhan, He, Pengfei, Munir, Shahzad, Li, Xingyu, Wu, Yixin, Wang, Junwei, Xia, Mengyuan, He, Pengbo, Wang, Ge, Yang, Huanwen, Karunarathna, Samantha C., Xie, Yan, He, Yueqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253258/
https://www.ncbi.nlm.nih.gov/pubmed/34222035
http://dx.doi.org/10.3389/fcimb.2021.598999
_version_ 1783717471769853952
author Jiao, Rong
Cai, Yongzhan
He, Pengfei
Munir, Shahzad
Li, Xingyu
Wu, Yixin
Wang, Junwei
Xia, Mengyuan
He, Pengbo
Wang, Ge
Yang, Huanwen
Karunarathna, Samantha C.
Xie, Yan
He, Yueqiu
author_facet Jiao, Rong
Cai, Yongzhan
He, Pengfei
Munir, Shahzad
Li, Xingyu
Wu, Yixin
Wang, Junwei
Xia, Mengyuan
He, Pengbo
Wang, Ge
Yang, Huanwen
Karunarathna, Samantha C.
Xie, Yan
He, Yueqiu
author_sort Jiao, Rong
collection PubMed
description Bacillus amyloliquefaciens YN201732 is an endophytic bacteria with high biocontrol efficiency and broad-spectrum antimicrobial activities. In order to clarify the main active ingredients and their antifungal mechanisms against powdery mildew of tobacco, this study is focused on lipopeptide obtained through acid precipitation and organic solvent extraction. HPLC and LCMS-IT-TOF were used to separate and identify antimicrobial lipopeptides. Findings revealed that bacillomycin D plays an important role against surrogate fungal pathogen Fusarium solani. Synthetic pathways of sfp, bacillomycin D, and fengycin were separately disrupted. The sfp gene knockout mutant B. amyloliquefaciens YN201732M1 only showed minor antagonistic activity against F. solani. While Erysiphe cichoracearum spore germination was inhibited and pot experiments displayed a significant decrease in tobacco powdery mildew. The spore inhibition rate of YN201732M1 was only 30.29%, and the pot experiment control effect was less than 37.39%, which was significantly lower than that of the wild type. The inhibitory effect of mutant YN201732M2 (deficient in the production of bacillomycin D) and mutant YN201732M3 (deficient in the production of fengycin) on the spore germination of E. cichoracearum were 50.22% and 53.06%, respectively, suggesting that both fengycin and bacillomycin D had potential effects on spore germination of powdery mildew. Interestingly, in a greenhouse assay, both B. amyloliquefaciens YN201732M2 and YN201732M3 mutants displayed less of a control effect on tobacco powdery mildew than wild type. The results from in vitro, spore germination, and greenhouse-pot studies demonstrated that antimicrobial lipopeptides especially bacillomycin D and fengycin may contribute to the prevention and control of tobacco powdery mildew. In addition, gene mutation related to lipopeptide synthesis can also affect the biofilm formation of strains.
format Online
Article
Text
id pubmed-8253258
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82532582021-07-03 Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum Jiao, Rong Cai, Yongzhan He, Pengfei Munir, Shahzad Li, Xingyu Wu, Yixin Wang, Junwei Xia, Mengyuan He, Pengbo Wang, Ge Yang, Huanwen Karunarathna, Samantha C. Xie, Yan He, Yueqiu Front Cell Infect Microbiol Cellular and Infection Microbiology Bacillus amyloliquefaciens YN201732 is an endophytic bacteria with high biocontrol efficiency and broad-spectrum antimicrobial activities. In order to clarify the main active ingredients and their antifungal mechanisms against powdery mildew of tobacco, this study is focused on lipopeptide obtained through acid precipitation and organic solvent extraction. HPLC and LCMS-IT-TOF were used to separate and identify antimicrobial lipopeptides. Findings revealed that bacillomycin D plays an important role against surrogate fungal pathogen Fusarium solani. Synthetic pathways of sfp, bacillomycin D, and fengycin were separately disrupted. The sfp gene knockout mutant B. amyloliquefaciens YN201732M1 only showed minor antagonistic activity against F. solani. While Erysiphe cichoracearum spore germination was inhibited and pot experiments displayed a significant decrease in tobacco powdery mildew. The spore inhibition rate of YN201732M1 was only 30.29%, and the pot experiment control effect was less than 37.39%, which was significantly lower than that of the wild type. The inhibitory effect of mutant YN201732M2 (deficient in the production of bacillomycin D) and mutant YN201732M3 (deficient in the production of fengycin) on the spore germination of E. cichoracearum were 50.22% and 53.06%, respectively, suggesting that both fengycin and bacillomycin D had potential effects on spore germination of powdery mildew. Interestingly, in a greenhouse assay, both B. amyloliquefaciens YN201732M2 and YN201732M3 mutants displayed less of a control effect on tobacco powdery mildew than wild type. The results from in vitro, spore germination, and greenhouse-pot studies demonstrated that antimicrobial lipopeptides especially bacillomycin D and fengycin may contribute to the prevention and control of tobacco powdery mildew. In addition, gene mutation related to lipopeptide synthesis can also affect the biofilm formation of strains. Frontiers Media S.A. 2021-06-18 /pmc/articles/PMC8253258/ /pubmed/34222035 http://dx.doi.org/10.3389/fcimb.2021.598999 Text en Copyright © 2021 Jiao, Cai, He, Munir, Li, Wu, Wang, Xia, He, Wang, Yang, Karunarathna, Xie and He https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Jiao, Rong
Cai, Yongzhan
He, Pengfei
Munir, Shahzad
Li, Xingyu
Wu, Yixin
Wang, Junwei
Xia, Mengyuan
He, Pengbo
Wang, Ge
Yang, Huanwen
Karunarathna, Samantha C.
Xie, Yan
He, Yueqiu
Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum
title Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum
title_full Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum
title_fullStr Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum
title_full_unstemmed Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum
title_short Bacillus amyloliquefaciens YN201732 Produces Lipopeptides With Promising Biocontrol Activity Against Fungal Pathogen Erysiphe cichoracearum
title_sort bacillus amyloliquefaciens yn201732 produces lipopeptides with promising biocontrol activity against fungal pathogen erysiphe cichoracearum
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253258/
https://www.ncbi.nlm.nih.gov/pubmed/34222035
http://dx.doi.org/10.3389/fcimb.2021.598999
work_keys_str_mv AT jiaorong bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT caiyongzhan bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT hepengfei bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT munirshahzad bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT lixingyu bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT wuyixin bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT wangjunwei bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT xiamengyuan bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT hepengbo bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT wangge bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT yanghuanwen bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT karunarathnasamanthac bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT xieyan bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum
AT heyueqiu bacillusamyloliquefaciensyn201732produceslipopeptideswithpromisingbiocontrolactivityagainstfungalpathogenerysiphecichoracearum