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Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases

Genome-wide identification of DNA double-strand breaks (DSBs) induced by CRISPR-associated protein (Cas) systems is vital for profiling the off-target events of Cas nucleases. However, current methods for off-target discovery are tedious and costly, restricting their widespread applications. Here we...

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Detalles Bibliográficos
Autores principales:	Huang, Hongxin, Hu, Yongfei, Huang, Guanjie, Ma, Shufeng, Feng, Jianqi, Wang, Dong, Lin, Ying, Zhou, Jiajian, Rong, Zhili
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253812/ https://www.ncbi.nlm.nih.gov/pubmed/34215845 http://dx.doi.org/10.1038/s42003-021-02351-3

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