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Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions

For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO(2)) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics we...

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Detalles Bibliográficos
Autores principales: Gomez-Gomez, Angela, Brito-de la Fuente, Edmundo, Gallegos, Críspulo, Garcia-Perez, Jose V., Benedito, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254120/
https://www.ncbi.nlm.nih.gov/pubmed/34192660
http://dx.doi.org/10.1016/j.ultsonch.2021.105636
Descripción
Sumario:For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO(2)) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics were analyzed at different pressures (100, 350 and 550 bar) and temperatures (50, 60, 70, 80, 85 °C), depending on the microorganism, and compared to the conventional thermal treatment. The inactivation kinetics were satisfactorily described using the Weibull model. Experimental results showed that SC-CO(2) enhanced the inactivation level of both spores when compared to thermal treatments. Bacterial spores (C. butyricum) were found to be more resistant to SC-CO(2) + HPU, than fungal (A. niger) ones, as also observed in the thermal and SC-CO(2) treatments. The application of HPU intensified the SC-CO(2) inactivation of C. butyricum spores, e.g. shortening the total inactivation time from 10 to 3 min at 85 °C. However, HPU did not affect the SC-CO(2) inactivation of A. niger spores. The study into the effect of a combined SC-CO(2) + HPU treatment has to be necessarily extended to other fungal and bacterial spores, and future studies should elucidate the impact of HPU application on the emulsion’s stability.