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Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions

For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO(2)) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics we...

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Autores principales: Gomez-Gomez, Angela, Brito-de la Fuente, Edmundo, Gallegos, Críspulo, Garcia-Perez, Jose V., Benedito, Jose
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254120/
https://www.ncbi.nlm.nih.gov/pubmed/34192660
http://dx.doi.org/10.1016/j.ultsonch.2021.105636
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author Gomez-Gomez, Angela
Brito-de la Fuente, Edmundo
Gallegos, Críspulo
Garcia-Perez, Jose V.
Benedito, Jose
author_facet Gomez-Gomez, Angela
Brito-de la Fuente, Edmundo
Gallegos, Críspulo
Garcia-Perez, Jose V.
Benedito, Jose
author_sort Gomez-Gomez, Angela
collection PubMed
description For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO(2)) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics were analyzed at different pressures (100, 350 and 550 bar) and temperatures (50, 60, 70, 80, 85 °C), depending on the microorganism, and compared to the conventional thermal treatment. The inactivation kinetics were satisfactorily described using the Weibull model. Experimental results showed that SC-CO(2) enhanced the inactivation level of both spores when compared to thermal treatments. Bacterial spores (C. butyricum) were found to be more resistant to SC-CO(2) + HPU, than fungal (A. niger) ones, as also observed in the thermal and SC-CO(2) treatments. The application of HPU intensified the SC-CO(2) inactivation of C. butyricum spores, e.g. shortening the total inactivation time from 10 to 3 min at 85 °C. However, HPU did not affect the SC-CO(2) inactivation of A. niger spores. The study into the effect of a combined SC-CO(2) + HPU treatment has to be necessarily extended to other fungal and bacterial spores, and future studies should elucidate the impact of HPU application on the emulsion’s stability.
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spelling pubmed-82541202021-07-12 Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions Gomez-Gomez, Angela Brito-de la Fuente, Edmundo Gallegos, Críspulo Garcia-Perez, Jose V. Benedito, Jose Ultrason Sonochem Ultrasound hybridized technologies: A new breathing for Sonochemistry For the first time, this study addresses the intensification of supercritical carbon dioxide (SC-CO(2)) treatments using high-power ultrasound (HPU) for the inactivation of fungal (Aspergillus niger) and bacterial (Clostridium butyricum) spores in oil-in-water emulsions. The inactivation kinetics were analyzed at different pressures (100, 350 and 550 bar) and temperatures (50, 60, 70, 80, 85 °C), depending on the microorganism, and compared to the conventional thermal treatment. The inactivation kinetics were satisfactorily described using the Weibull model. Experimental results showed that SC-CO(2) enhanced the inactivation level of both spores when compared to thermal treatments. Bacterial spores (C. butyricum) were found to be more resistant to SC-CO(2) + HPU, than fungal (A. niger) ones, as also observed in the thermal and SC-CO(2) treatments. The application of HPU intensified the SC-CO(2) inactivation of C. butyricum spores, e.g. shortening the total inactivation time from 10 to 3 min at 85 °C. However, HPU did not affect the SC-CO(2) inactivation of A. niger spores. The study into the effect of a combined SC-CO(2) + HPU treatment has to be necessarily extended to other fungal and bacterial spores, and future studies should elucidate the impact of HPU application on the emulsion’s stability. Elsevier 2021-06-19 /pmc/articles/PMC8254120/ /pubmed/34192660 http://dx.doi.org/10.1016/j.ultsonch.2021.105636 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Ultrasound hybridized technologies: A new breathing for Sonochemistry
Gomez-Gomez, Angela
Brito-de la Fuente, Edmundo
Gallegos, Críspulo
Garcia-Perez, Jose V.
Benedito, Jose
Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions
title Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions
title_full Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions
title_fullStr Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions
title_full_unstemmed Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions
title_short Combination of supercritical CO(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions
title_sort combination of supercritical co(2) and high-power ultrasound for the inactivation of fungal and bacterial spores in lipid emulsions
topic Ultrasound hybridized technologies: A new breathing for Sonochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254120/
https://www.ncbi.nlm.nih.gov/pubmed/34192660
http://dx.doi.org/10.1016/j.ultsonch.2021.105636
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