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Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China

BACKGROUND: Human papillomavirus (HPV) type 16 accounts for a larger share of cervical cancer and has been a major health problem worldwide for decades. The progression of initial infection to cervical cancer has been linked to viral sequence properties; however, the role of HPV16 variants in the ri...

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Autores principales: Dai, Mei-Zhen, Qiu, Yi, Di, Xing-Hong, Shi, Wei-Wu, Xu, Hui-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254333/
https://www.ncbi.nlm.nih.gov/pubmed/34217247
http://dx.doi.org/10.1186/s12885-021-08531-y
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author Dai, Mei-Zhen
Qiu, Yi
Di, Xing-Hong
Shi, Wei-Wu
Xu, Hui-Hui
author_facet Dai, Mei-Zhen
Qiu, Yi
Di, Xing-Hong
Shi, Wei-Wu
Xu, Hui-Hui
author_sort Dai, Mei-Zhen
collection PubMed
description BACKGROUND: Human papillomavirus (HPV) type 16 accounts for a larger share of cervical cancer and has been a major health problem worldwide for decades. The progression of initial infection to cervical cancer has been linked to viral sequence properties; however, the role of HPV16 variants in the risk of cervical carcinogenesis, especially with longitudinal follow-up, is not fully understood in China. METHODS: We aimed to investigate the genetic variability of HPV16 E6 and E7 oncogenes in isolates from cervical exfoliated cells. Between December 2012 and December 2014, a total of 310 single HPV16-positive samples were selected from women living in the Taizhou area, China. Sequences of all E6 and E7 oncogenes were analysed by PCR-sequencing assay. Detailed sequence comparison, genetic heterogeneity analyses and maximum-likelihood phylogenetic tree construction were performed with BioEdit Sequence Alignment Editor and MEGA X software. Data for cytology tests and histological diagnoses were obtained from our Taizhou Area Study with longitudinal follow-up for at least 5 years. The relationship between HPV16 variants and cervical carcinogenesis risk was analysed by the chi-square test or Fisher’s exact test. RESULTS: In this study, we obtained 64 distinct variation patterns with the accession GenBank numbers MT681266-MT681329. Phylogenetic analysis revealed that 98.3% of HPV16 variants belong to lineage A, in which the A4 (Asian) sublineage was dominant (64.8%), followed by A2 (12.1%), A1 (11.4%), and A3 (10.0%). The A4 (Asian) sublineage had a higher risk of CIN2+ than the A1–3 (European) sublineages (OR = 2.69, 95% CI = 1.04–6.97, P < 0.05). Furthermore, nucleotide variation in HPV16 E6 T178G is associated with the development of cervical cancer. CONCLUSION: These data could provide novel insights into the role of HPV16 variants in cervical carcinogenesis risk in China. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-021-08531-y.
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spelling pubmed-82543332021-07-06 Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China Dai, Mei-Zhen Qiu, Yi Di, Xing-Hong Shi, Wei-Wu Xu, Hui-Hui BMC Cancer Research Article BACKGROUND: Human papillomavirus (HPV) type 16 accounts for a larger share of cervical cancer and has been a major health problem worldwide for decades. The progression of initial infection to cervical cancer has been linked to viral sequence properties; however, the role of HPV16 variants in the risk of cervical carcinogenesis, especially with longitudinal follow-up, is not fully understood in China. METHODS: We aimed to investigate the genetic variability of HPV16 E6 and E7 oncogenes in isolates from cervical exfoliated cells. Between December 2012 and December 2014, a total of 310 single HPV16-positive samples were selected from women living in the Taizhou area, China. Sequences of all E6 and E7 oncogenes were analysed by PCR-sequencing assay. Detailed sequence comparison, genetic heterogeneity analyses and maximum-likelihood phylogenetic tree construction were performed with BioEdit Sequence Alignment Editor and MEGA X software. Data for cytology tests and histological diagnoses were obtained from our Taizhou Area Study with longitudinal follow-up for at least 5 years. The relationship between HPV16 variants and cervical carcinogenesis risk was analysed by the chi-square test or Fisher’s exact test. RESULTS: In this study, we obtained 64 distinct variation patterns with the accession GenBank numbers MT681266-MT681329. Phylogenetic analysis revealed that 98.3% of HPV16 variants belong to lineage A, in which the A4 (Asian) sublineage was dominant (64.8%), followed by A2 (12.1%), A1 (11.4%), and A3 (10.0%). The A4 (Asian) sublineage had a higher risk of CIN2+ than the A1–3 (European) sublineages (OR = 2.69, 95% CI = 1.04–6.97, P < 0.05). Furthermore, nucleotide variation in HPV16 E6 T178G is associated with the development of cervical cancer. CONCLUSION: These data could provide novel insights into the role of HPV16 variants in cervical carcinogenesis risk in China. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-021-08531-y. BioMed Central 2021-07-03 /pmc/articles/PMC8254333/ /pubmed/34217247 http://dx.doi.org/10.1186/s12885-021-08531-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Dai, Mei-Zhen
Qiu, Yi
Di, Xing-Hong
Shi, Wei-Wu
Xu, Hui-Hui
Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China
title Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China
title_full Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China
title_fullStr Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China
title_full_unstemmed Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China
title_short Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China
title_sort association of cervical carcinogenesis risk with hpv16 e6 and e7 variants in the taizhou area, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254333/
https://www.ncbi.nlm.nih.gov/pubmed/34217247
http://dx.doi.org/10.1186/s12885-021-08531-y
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