Cargando…
Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048
With an unsupervised GC–MS metabolomics approach, polar metabolite changes of the microalgae Coccomyxa melkonianii SCCA 048 grown under standard conditions for seven weeks were studied. C. melkonianii was sampled at the Rio Irvi River, in the mining site of Montevecchio-Ingurtosu (Sardinia, Italy),...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Japan
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254698/ https://www.ncbi.nlm.nih.gov/pubmed/34057605 http://dx.doi.org/10.1007/s00792-021-01234-z |
_version_ | 1783717777972920320 |
---|---|
author | Fais, Giacomo Malavasi, Veronica Scano, Paola Soru, Santina Caboni, Pierluigi Cao, Giacomo |
author_facet | Fais, Giacomo Malavasi, Veronica Scano, Paola Soru, Santina Caboni, Pierluigi Cao, Giacomo |
author_sort | Fais, Giacomo |
collection | PubMed |
description | With an unsupervised GC–MS metabolomics approach, polar metabolite changes of the microalgae Coccomyxa melkonianii SCCA 048 grown under standard conditions for seven weeks were studied. C. melkonianii was sampled at the Rio Irvi River, in the mining site of Montevecchio-Ingurtosu (Sardinia, Italy), which is severely contaminated by heavy metals and shows high concentrations of sulfates. The partial-least-square (PLS) analysis of the GC–MS data indicated that growth of C. melkonianii was characterized by an increase of the levels of threonic acid, myo-inositol, malic acid, and fumaric acid. Furthermore, at the sixth week of exponential phase the lipid fingerprint of C. melkonianii was studied by LC-QTOF-MS. C. melkonianii lipid extract characterized through an iterative MS/MS analysis showed the following percent levels: 61.34 ± 0.60% for triacylglycerols (TAG); 11.55 ± 0.09% for diacylglyceryltrimethyl homoserines (DGTS), 11.34 ± 0.10% for sulfoquinovosyldiacylglycerols (SQDG) and, 5.29 ± 0.04% for lysodiacylglyceryltrimethyl homoserines (LDGTS). Noteworthy, we were able to annotate different fatty acid ester of hydroxyl fatty acid, such as FAHFA (18:1_20:3), FAHFA (18:2_20:4), FAHFA (18:0_20:2), and FAHFA (18:1_18:0), with relevant biological activity. These approaches can be useful to study the biochemistry of this extremophile algae in the view of its potential exploitation in the phycoremediation of polluted mining areas. |
format | Online Article Text |
id | pubmed-8254698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-82546982021-07-20 Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 Fais, Giacomo Malavasi, Veronica Scano, Paola Soru, Santina Caboni, Pierluigi Cao, Giacomo Extremophiles Original Paper With an unsupervised GC–MS metabolomics approach, polar metabolite changes of the microalgae Coccomyxa melkonianii SCCA 048 grown under standard conditions for seven weeks were studied. C. melkonianii was sampled at the Rio Irvi River, in the mining site of Montevecchio-Ingurtosu (Sardinia, Italy), which is severely contaminated by heavy metals and shows high concentrations of sulfates. The partial-least-square (PLS) analysis of the GC–MS data indicated that growth of C. melkonianii was characterized by an increase of the levels of threonic acid, myo-inositol, malic acid, and fumaric acid. Furthermore, at the sixth week of exponential phase the lipid fingerprint of C. melkonianii was studied by LC-QTOF-MS. C. melkonianii lipid extract characterized through an iterative MS/MS analysis showed the following percent levels: 61.34 ± 0.60% for triacylglycerols (TAG); 11.55 ± 0.09% for diacylglyceryltrimethyl homoserines (DGTS), 11.34 ± 0.10% for sulfoquinovosyldiacylglycerols (SQDG) and, 5.29 ± 0.04% for lysodiacylglyceryltrimethyl homoserines (LDGTS). Noteworthy, we were able to annotate different fatty acid ester of hydroxyl fatty acid, such as FAHFA (18:1_20:3), FAHFA (18:2_20:4), FAHFA (18:0_20:2), and FAHFA (18:1_18:0), with relevant biological activity. These approaches can be useful to study the biochemistry of this extremophile algae in the view of its potential exploitation in the phycoremediation of polluted mining areas. Springer Japan 2021-05-31 2021 /pmc/articles/PMC8254698/ /pubmed/34057605 http://dx.doi.org/10.1007/s00792-021-01234-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Paper Fais, Giacomo Malavasi, Veronica Scano, Paola Soru, Santina Caboni, Pierluigi Cao, Giacomo Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 |
title | Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 |
title_full | Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 |
title_fullStr | Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 |
title_full_unstemmed | Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 |
title_short | Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048 |
title_sort | metabolomics and lipid profile analysis of coccomyxa melkonianii scca 048 |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254698/ https://www.ncbi.nlm.nih.gov/pubmed/34057605 http://dx.doi.org/10.1007/s00792-021-01234-z |
work_keys_str_mv | AT faisgiacomo metabolomicsandlipidprofileanalysisofcoccomyxamelkonianiiscca048 AT malavasiveronica metabolomicsandlipidprofileanalysisofcoccomyxamelkonianiiscca048 AT scanopaola metabolomicsandlipidprofileanalysisofcoccomyxamelkonianiiscca048 AT sorusantina metabolomicsandlipidprofileanalysisofcoccomyxamelkonianiiscca048 AT cabonipierluigi metabolomicsandlipidprofileanalysisofcoccomyxamelkonianiiscca048 AT caogiacomo metabolomicsandlipidprofileanalysisofcoccomyxamelkonianiiscca048 |