Cargando…
Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice
Dynamic changes in adipose tissue blood flow (ATBF) with nutritional status play a role in the regulation of metabolic and endocrine functions. Activation of the sympathetic nervous system via β-adrenergic receptors (β-AR) contributes to the control of postprandial enhancement of ATBF. Herein, we so...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Physiological Society and The Korean Society of Pharmacology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255121/ https://www.ncbi.nlm.nih.gov/pubmed/34187952 http://dx.doi.org/10.4196/kjpp.2021.25.4.355 |
_version_ | 1783717845847244800 |
---|---|
author | Lee, Hye-Jin Jin, Bo-Yeong Park, Mi-Rae Seo, Kwan Sik Jeong, Yong Taek Choi, Sang-Hyun Kim, Dong-Hoon |
author_facet | Lee, Hye-Jin Jin, Bo-Yeong Park, Mi-Rae Seo, Kwan Sik Jeong, Yong Taek Choi, Sang-Hyun Kim, Dong-Hoon |
author_sort | Lee, Hye-Jin |
collection | PubMed |
description | Dynamic changes in adipose tissue blood flow (ATBF) with nutritional status play a role in the regulation of metabolic and endocrine functions. Activation of the sympathetic nervous system via β-adrenergic receptors (β-AR) contributes to the control of postprandial enhancement of ATBF. Herein, we sought to identify the role of each β-AR subtype in the regulation of ATBF in mice. We monitored the changes in visceral epididymal ATBF (VAT BF), induced by local infusion of dobutamine, salbutamol, and CL316,243 (a selective β1-, β2-, and β3-AR agonist, respectively) into VAT of lean CD-1 mice and global adipose triglyceride lipase (ATGL) knockout (KO) mice, using laser Doppler flowmetry. Administration of CL316,243, known to promote lipolysis in adipocytes, significantly increased VAT BF of CD-1 mice to a greater extent compared to that of the vehicle, whereas administration of dobutamine or salbutamol did not produce significant differences in VAT BF. The increase in VAT BF induced by β3-AR stimulation disappeared in ATGL KO mice as opposed to their wild-type (WT) littermates, implying a role of ATGL-mediated lipolysis in the regulation of VAT BF. Different vascular reactivities occurred despite no significant differences in vessel density and adiposity between the groups. Additionally, the expression levels of the angiogenesis-related genes were significantly higher in VAT of ATGL KO mice than in that of WT, implicating an association of ATBF responsiveness with angiogenic activity in VAT. Our findings suggest a potential role of β3-AR signaling in the regulation of VAT BF via ATGL-mediated lipolysis in mice. |
format | Online Article Text |
id | pubmed-8255121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-82551212021-07-15 Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice Lee, Hye-Jin Jin, Bo-Yeong Park, Mi-Rae Seo, Kwan Sik Jeong, Yong Taek Choi, Sang-Hyun Kim, Dong-Hoon Korean J Physiol Pharmacol Original Article Dynamic changes in adipose tissue blood flow (ATBF) with nutritional status play a role in the regulation of metabolic and endocrine functions. Activation of the sympathetic nervous system via β-adrenergic receptors (β-AR) contributes to the control of postprandial enhancement of ATBF. Herein, we sought to identify the role of each β-AR subtype in the regulation of ATBF in mice. We monitored the changes in visceral epididymal ATBF (VAT BF), induced by local infusion of dobutamine, salbutamol, and CL316,243 (a selective β1-, β2-, and β3-AR agonist, respectively) into VAT of lean CD-1 mice and global adipose triglyceride lipase (ATGL) knockout (KO) mice, using laser Doppler flowmetry. Administration of CL316,243, known to promote lipolysis in adipocytes, significantly increased VAT BF of CD-1 mice to a greater extent compared to that of the vehicle, whereas administration of dobutamine or salbutamol did not produce significant differences in VAT BF. The increase in VAT BF induced by β3-AR stimulation disappeared in ATGL KO mice as opposed to their wild-type (WT) littermates, implying a role of ATGL-mediated lipolysis in the regulation of VAT BF. Different vascular reactivities occurred despite no significant differences in vessel density and adiposity between the groups. Additionally, the expression levels of the angiogenesis-related genes were significantly higher in VAT of ATGL KO mice than in that of WT, implicating an association of ATBF responsiveness with angiogenic activity in VAT. Our findings suggest a potential role of β3-AR signaling in the regulation of VAT BF via ATGL-mediated lipolysis in mice. The Korean Physiological Society and The Korean Society of Pharmacology 2021-07-01 2021-07-01 /pmc/articles/PMC8255121/ /pubmed/34187952 http://dx.doi.org/10.4196/kjpp.2021.25.4.355 Text en Copyright © Korean J Physiol Pharmacol https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lee, Hye-Jin Jin, Bo-Yeong Park, Mi-Rae Seo, Kwan Sik Jeong, Yong Taek Choi, Sang-Hyun Kim, Dong-Hoon Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice |
title | Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice |
title_full | Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice |
title_fullStr | Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice |
title_full_unstemmed | Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice |
title_short | Deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice |
title_sort | deletion of adipose triglyceride lipase abolishes blood flow increase after β3-adrenergic stimulation in visceral adipose tissue of mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255121/ https://www.ncbi.nlm.nih.gov/pubmed/34187952 http://dx.doi.org/10.4196/kjpp.2021.25.4.355 |
work_keys_str_mv | AT leehyejin deletionofadiposetriglyceridelipaseabolishesbloodflowincreaseafterb3adrenergicstimulationinvisceraladiposetissueofmice AT jinboyeong deletionofadiposetriglyceridelipaseabolishesbloodflowincreaseafterb3adrenergicstimulationinvisceraladiposetissueofmice AT parkmirae deletionofadiposetriglyceridelipaseabolishesbloodflowincreaseafterb3adrenergicstimulationinvisceraladiposetissueofmice AT seokwansik deletionofadiposetriglyceridelipaseabolishesbloodflowincreaseafterb3adrenergicstimulationinvisceraladiposetissueofmice AT jeongyongtaek deletionofadiposetriglyceridelipaseabolishesbloodflowincreaseafterb3adrenergicstimulationinvisceraladiposetissueofmice AT choisanghyun deletionofadiposetriglyceridelipaseabolishesbloodflowincreaseafterb3adrenergicstimulationinvisceraladiposetissueofmice AT kimdonghoon deletionofadiposetriglyceridelipaseabolishesbloodflowincreaseafterb3adrenergicstimulationinvisceraladiposetissueofmice |