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Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients

Hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a new biomarker that reflects HBV replication, but its relationship with natural killer (NK) cell immunity in chronic hepatitis B (CHB) is unknown. We assessed serum HBV pgRNA levels in 323 CHB patients by reverse transcription-polymerase chain react...

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Autores principales: Gu, Yurong, Huang, Zexuan, Li, Xiaoyan, Chen, Youming, Liao, Chunhong, Bi, Yanhua, Huang, Yuehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255538/
https://www.ncbi.nlm.nih.gov/pubmed/34151357
http://dx.doi.org/10.1042/BSR20210600
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author Gu, Yurong
Huang, Zexuan
Li, Xiaoyan
Chen, Youming
Liao, Chunhong
Bi, Yanhua
Huang, Yuehua
author_facet Gu, Yurong
Huang, Zexuan
Li, Xiaoyan
Chen, Youming
Liao, Chunhong
Bi, Yanhua
Huang, Yuehua
author_sort Gu, Yurong
collection PubMed
description Hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a new biomarker that reflects HBV replication, but its relationship with natural killer (NK) cell immunity in chronic hepatitis B (CHB) is unknown. We assessed serum HBV pgRNA levels in 323 CHB patients by reverse transcription-polymerase chain reaction, assessed cytokine production and activation and inhibitory markers of NK cells by flow cytometry, and measured serum cytokines by enzyme-linked immunosorbent assays (ELISAs). Among the different CHB phases, the serum HBV pgRNA level was highest in the immune-tolerant (IT) and immune-active (IA) phases. Regarding NK and NK(dim) cells, HBV pgRNA was negatively associated with frequencies, but positively associated with NKp44 and NKp46 expression (activation markers). Regarding NK(bright) cells, serum HBV pgRNA was positively associated with frequency and programmed cell death protein 1 (PD1) expression (inhibitory marker), but negatively associated with NKp44 and NKp46. Serum HBV pgRNA was not associated with NKp30 (activation marker) on NK cells or subsets. Lastly, serum HBV pgRNA was positively correlated with the levels of serum IL-7 and IL-12P40 (NK cell-promoting cytokines) and negatively correlated with serum prostaglandin E2 (PGE2) level (which negatively regulates NK cells). In conclusion, we found varied relationships between serum HBV pgRNA and NK cells and subsets, indicating that HBV pgRNA may play a complicated role in NK cell-related immunity, providing new information on HBV and host immunity.
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spelling pubmed-82555382021-07-16 Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients Gu, Yurong Huang, Zexuan Li, Xiaoyan Chen, Youming Liao, Chunhong Bi, Yanhua Huang, Yuehua Biosci Rep Virology Hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a new biomarker that reflects HBV replication, but its relationship with natural killer (NK) cell immunity in chronic hepatitis B (CHB) is unknown. We assessed serum HBV pgRNA levels in 323 CHB patients by reverse transcription-polymerase chain reaction, assessed cytokine production and activation and inhibitory markers of NK cells by flow cytometry, and measured serum cytokines by enzyme-linked immunosorbent assays (ELISAs). Among the different CHB phases, the serum HBV pgRNA level was highest in the immune-tolerant (IT) and immune-active (IA) phases. Regarding NK and NK(dim) cells, HBV pgRNA was negatively associated with frequencies, but positively associated with NKp44 and NKp46 expression (activation markers). Regarding NK(bright) cells, serum HBV pgRNA was positively associated with frequency and programmed cell death protein 1 (PD1) expression (inhibitory marker), but negatively associated with NKp44 and NKp46. Serum HBV pgRNA was not associated with NKp30 (activation marker) on NK cells or subsets. Lastly, serum HBV pgRNA was positively correlated with the levels of serum IL-7 and IL-12P40 (NK cell-promoting cytokines) and negatively correlated with serum prostaglandin E2 (PGE2) level (which negatively regulates NK cells). In conclusion, we found varied relationships between serum HBV pgRNA and NK cells and subsets, indicating that HBV pgRNA may play a complicated role in NK cell-related immunity, providing new information on HBV and host immunity. Portland Press Ltd. 2021-07-02 /pmc/articles/PMC8255538/ /pubmed/34151357 http://dx.doi.org/10.1042/BSR20210600 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Virology
Gu, Yurong
Huang, Zexuan
Li, Xiaoyan
Chen, Youming
Liao, Chunhong
Bi, Yanhua
Huang, Yuehua
Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients
title Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients
title_full Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients
title_fullStr Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients
title_full_unstemmed Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients
title_short Serum HBV pregenomic RNA exhibited opposite associations with NK(dim) and NK(bright) cell immunity in treatment-naïve chronic hepatitis B patients
title_sort serum hbv pregenomic rna exhibited opposite associations with nk(dim) and nk(bright) cell immunity in treatment-naïve chronic hepatitis b patients
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255538/
https://www.ncbi.nlm.nih.gov/pubmed/34151357
http://dx.doi.org/10.1042/BSR20210600
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